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| Forti,Fábio L.; Costa,Érico T.; Rocha,Kátia M.; Moraes,Miriam S.; Armelin,Hugo A.. |
| The mouse Y1 adrenocortical tumor cell line is highly responsive to FGF2-(Fibroblast Growth Factor 2) and possesses amplified and over-expressed c-Ki-ras proto-oncogene. We previously reported that this genetic lesion leads to high constitutive levels of activation of the c-Ki-Ras-GTP->PI3K->Akt signaling pathway (Forti et al. 2002). On the other hand, activation levels of another important pathway downstream of c-Ki-Ras-GTP, namely, Raf->MEK->ERK, remain strictly dependent on FGF2 stimulation (Rocha et al. 2003). Here we show that, first, FGF2 transiently up-regulates the c-Ki-Ras-GTP->PI3K->Akt pathway, in spite of its high basal levels. Second, c-Ki-Ras-GTP transient up-regulation likely underlies activation of the ERK1/2 pathway by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: FGF2; Adrenocortical tumor cells; C-ki-ras; C-h-ras; ERK. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000200005 |
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| Zhang,Lei-Yi; Zhou,Yue-Ying; Chen,Fei; Wang,Bing; Li,Jing; Deng,You-Wen; Liu,Wei-Dong; Wang,Zheng-Guang; Li,Ya-Wei; Li,Dong-Zhe; Lv,Guo-Hua; Yin,Bang-Liang. |
| Taurine has positive effects on bone metabolism. However, the effects of taurine on osteoblast apoptosis in vitro have not been reported. The aim of this study was to investigate the activity of taurine on apoptosis of mouse osteoblastic MC3T3-E1 cells. The data showed that 1, 5, 10, or 20 mM taurine resulted in 16.7, 34.2, 66.9, or 63.75% reduction of MC3T3-E1 cell apoptosis induced by the serum deprivation (serum-free α-MEM), respectively. Taurine (1, 5, or 10 mM) also reduced cytochrome c release and inhibited activation of caspase-3 and -9, which were measured using fluorogenic substrates for caspase-3/caspase-9, in serum-deprived MC3T3-E1 cells. Furthermore, taurine (10 mM) induced extracellular signal-regulated kinase (ERK) phosphorylation in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Taurine; Osteoblasts; Apoptosis; Taurine transporter; ERK. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700002 |
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