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Registros recuperados: 6
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Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line BJMBR
Gimba,E.R.P.; Chemale,G.; Farias,S.S.; Zaha,A..
We report here for the first time the structure and function of a promoter from a cestode. The ability of DNA fragments respectively encompassing the 935-bp and 524-bp regions upstream from the ATG codon from the EgactI and EgactII actin genes of Echinococcus granulosus to promote transcription was studied in the NIH3T3 mouse cell line. The results of transfection assays showed that both regions have strong promoter activity in these cells. The fragments were tested in both orientations and the 524-bp fragment of EgactII presented a bidirectional promoter activity. Deletion analysis of EgactI and EgactII promoters indicated the presence of regulatory regions containing putative silencer elements. These results indicate that both EgactI and EgactII...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Echinococcus granulosus; Actin; Promoters; Cestode; Gene expression; Deletions.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100004
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Therapeutic effects of Sophora moorcroftiana alkaloids in combination with albendazole in mice experimentally infected with protoscolices of Echinococcus granulosus BJMBR
Ma,X.M.; Bao,G.SH.; Wan,J.M.; Liao,D.J.; Yin,SH.F.; Meng,X.Q.; Zhou,G.K.; Lu,X.M.; Li,H.Y..
The objective of the present study was to determine if the combination of alkaloids from Sophora moorcroftiana seeds and albendazole might be effective in the treatment of experimental echinococcosisin female NIH mice (6 weeks old and weighing 18-20 g, N = 8 in each group) infected withprotoscolices of Echinococcus granulosus. Viable protoscolices (N = 6 x 103) were cultured in vitro in 1640 medium and mortality was calculated daily. To determine the in vivo efficacy, mice were inoculated intraperitoneally with viable protoscolices and then treated once daily by gavage for three months with the alkaloids (50 mg kg-1 day-1) and albendazole (50 mg kg-1 day-1), separately and in combination (both alkaloids at 25 mg kg-1 day-1 and albendazole at 25 mg kg-1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sophora moorcroftiana; Echinococcus granulosus; Alkaloids; Cytokines; Ethnobotany; Therapeutics.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000014
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Cerebral equine hydatidosis in Southern Brazil Ciência Rural
Ruas,Jerônimo Lopes; Schild,Ana Lucia; Fernandes,Cristina Gevehr; Ferreira,João Luiz Montiel; Müller,Gertrud.
Equine cerebral hydatid disease is described in a 7-year-old, crossbred, female horse. Clinical signs were characterized by circling gait, pressing of head against fences or objects and motor incoordination. On gross examination of the brain the hemispheres were swollen, mainly the left one. On transversal sections, a 5cm X 7cm fluid-filled cyst was observed within the lateral ventricle of the left hemisphere. The cyst extended from the parietal to the occipital lobe, and compressed the third ventricle. There was also marked mid line deviation. Histologically, the brain lesion adjacent to the cyst, was characterized by a piogranulomatous process and vacuolization of neuropil. A diagnosis of equine hydatidosis caused by Echinococcus granulosus was made on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydatid disease; Horse; Echinococcus granulosus.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781997000200030
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Prevalência de Echinococcus granulosus (Batsch, 1786) em cães urbanos errantes do município de Dom Pedrito (RS), Brasil Ciência Rural
Hoffmann,Adriane Nunes; Malgor,Ramiro; Rue,Mário Luiz de la.
Echinococcus granulosus é um dos parasitas mais importantes envolvidos em zoonoses de municípios próximos a fronteira do Rio grande do Sul com a Argentina e Uruguai. Amostras de 65 cães urbanos errantes do município de Dom Pedrito foram analisadas por meio de três técnicas: purgação pelo bromidrato de arecolina para visualização da presença do parasito; ensaio de imunoadsorção enzimática (ELISA), para detecção de coproantígenos, imunofluorescência indireta (IFI), para detecção de anticorpos séricos contra E. granulosus. Destes cães, 7,7% (5/65) apresentaram o parasita nas fezes, 10,8 (7/65) coproantígenos e 13,8% (9/65) anticorpos séricos contra o cestódeo. Conclui-se que a equinococose canina, no meio urbano, pode representar um sério problema à saúde...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Echinococcus granulosus; Dom Pedrito; Prevalência; Cães urbanos errantes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000500017
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Molecular Epidemiology of Cystic Echinococcosis: Genotypic Characterization in Humans and Different Livestock International Journal of Morphology
Manterola,Carlos; Otzen,Tamara.
Echinococcus Granulosus (EG) is the major cause of cystic echinococcosis in humans and livestock in the world. In Chile is a zoonosis of great importance. The most frequently affected geographic areas are the Regions of Aysén, Los Rios, Los Lagos, Coquimbo and the Araucanía. Hence, it was discovered that in endemic areas of hydatidosis there could be several strains and genotypes of EG. In addition, there is evidence that some strains and genotypes are more infectious for human beings than others. This interesting phenomenon of the biology of EG has been studied using molecular biology techniques based on polymerase chain reaction (PCR) and DNA sequence analysis, which has made it possible to characterize the cestode species complex called EG sensu lato (s...
Tipo: Journal article Palavras-chave: Echinococcus granulosus; Genotypes; Cytochrome c oxidase subunit I; PCR-RAPD.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400048
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Caracterización y análisis de la actividad antigénica de proteínas de la membrana germinal de Echinococcus granulosus InVet
Prieto,L.H.; Fernández,E.D..
Proteínas presentes en un extracto de membrana germinal de Echinococcus granulosus fueron separadas en una fase acuosa y en una fase detergente mediante el tratamiento con Triton X-114. La caracterización mediante electroforesis en gel de poliacrilamida en presencia de dodecil sulfato de sodio, mostró que proteínas con pesos moleculares aproximados de 102, 45, 28.5-28 y 15.5-15 kDa se presentaron en el extracto y en la fase acuosa (proteínas hidrofílicas). La caracterización inmunológica de proteínas del extracto mediante inmunotransferencia frente a suero ovino no inmunizado, frente a suero ovino inmunizado con antígenos de E. granulosus y frente a suero anti-inmunoglobulina ovina, mostró patrones de reactividad similares. El ensayo inmunodetección en un...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Echinococcus granulosus; Membrana germinal; Proteínas; Actividad antigénica.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982006000100005
Registros recuperados: 6
Primeira ... 1 ... Última
 

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