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| Richini-Pereira,VB; Bosco,SMG; Theodoro,RC; Barrozo,L; Bagagli,E. |
| Road-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Road-killed animals; Fungal pathogens; Eco-epidemiology; PCR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400011 |
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| Pedrini,Sílvia Cristina Barboza; Rosa,Patrícia Sammarco; Medri,Ísis Meri; Mourão,Guilherme; Bagagli,Eduardo; Lopes,Carlos Alberto de Magalhães. |
| Leprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dasypus novemcinctus; Euphractus sexcinctus; Cabassous tatouay; Mycobacterium leprae; Eco-epidemiology; Wild mammals. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100010 |
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