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Viability and infectivity of an ectomycorrhizal inoculum produced in an airlift bioreactor and immobilized in calcium alginate BJM
Oliveira,Leyza Paloschi de; Rossi,Márcio José; Furigo Júnior,Agenor; Silva Filho,Germano Nunes; Oliveira,Vetúria Lopes de.
The viability and infectivity of an ectomycorrhizal inoculum (isolate UFSC-Rh90, Rhizopogon nigrescens), produced by submerged cultivation in an airlift bioreactor and immobilized in beads of calcium alginate gel, was studied. Inoculum remained 100% viable after 18 months in a 0.85% NaCl solution at 8 ± 1ºC. Mycelium grew from the beads after 48 h when they were placed on a solid culture medium at 25 ± 1ºC. Viability of pellets of non-immobilized mycelium stored under the same conditions decreased gradually after the third month of storage, reaching 0% by the 12th month. These pellets presented a gradual darkening, which was more intense in those located near the surface of the NaCl solution. In culture medium, these dark pellets showed no viability. Gel...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizopogon nigrescens; Ectomycorrhizal fungus; Submerged cultivation; Pinus taeda.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300011
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Copper resistance of different ectomycorrhizal fungi such as Pisolithus microcarpus, Pisolithus sp., Scleroderma sp. and Suillus sp. BJM
Silva,R.F.; Lupatini,M.; Trindade,L.; Antoniolli,Z.I.; Steffen,R.B.; Andreazza,R.
Environments contaminated with heavy metals negatively impact the living organisms. Ectomy­corrhizal fungi have shown important role in these impacted sites. Thus, this study aimed to evaluate the copper-resistance of ectomycorrhizal fungi isolates Pisolithus microcarpus - UFSC-Pt116; Pisolithus sp. - UFSC-PT24, Suillus sp. - UFSM RA 2.8 and Scleroderma sp. - UFSC-Sc124 to different copper doses in solid and liquid media. The copper doses tested were: 0.00, 0.25, 0.5, 0.75, 1.0 and 1.25 mmol L-1 in the solid medium and 0.00, 0.32, 0.64 and 0.96 mmol L-1 in the liquid medium. Copper was amended as copper sulphate in order to supplement the culture medium MNM at pH 4.8, with seven replicates to each fungus-dose combination. The fungal isolates were incubated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ectomycorrhizal fungus; Copper-resistance; Pisolithus microcarpus; Pisolithus sp.; Scleroderma sp.; Suillus sp.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200042
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Morphological and molecular characterization of Pisolithus in soil under eucalyptus plantations in Brazil Rev. Bras. Ciênc. Solo
Kasuya,Maria Catarina Megumi; Coelho,Irene da Silva; Campos,Daniela Tiago da Silva; Araújo,Elza Fernandes de; Tamai,Yutaka; Miyamoto,Toshizumi.
Eighteen Pisolithus basidiomes were collected from Eucalyptus plantations in the state of Minas Gerais, Brazil. These basidiomes were characterized morphologically and molecularly. The basidiomes varied in shape, color and size. One of them was found underground, indicating a hypogeous fungus. The main morphological distinctive characteristic was spore ornamentation, which distinguished two groups. One group with short and erect spines was identified as Pisolithus microcarpus, and the other with long and curved spines as Pisolithus marmoratus, after analyzing the cladogram obtained by phylogenetic relationship based on internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA of these isolates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ectomycorrhizal fungus; Phylogenetic analysis; Internal transcribed spacer (ITS); Nuclear ribosomal DNA; Taxonomy.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000600013
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