|
|
|
|
|
| Mahmood,Tariq; Zar,Tamkina; Saqlan Naqvi,S.M. |
| Electroporation entails brief, high intensity pulse to create transient pores in the cell membrane to facilitate the entry of exogenous macromolecules, which may otherwise be excluded. Removal of the external field leads to the resealing of the membrane electropores permitting the survival of the electrically stimulated recipient cells. Using this technique foreign deoxyribonucleic acid (DNA) has been successfully introduced into many cell types both from prokaryotes and eukaryotes. Increase in pulse voltage and length beyond a critical limit has been reported to decrease transformation efficiency, hence in this study we have investigated another strategy i.e. increase in the number of pulses at constant high voltage and pulse duration. Commonly used... |
| Tipo: Journal article |
Palavras-chave: Agrobacterium; Electroporation; Pulse number; Transformation efficiency. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100015 |
| |
|
| |
|
|
| Alves,Adriana Cristina; Quecini,Vera Maria; Vieira,Maria Lucia Carneiro. |
| Genetic transformation is a powerful tool for plant breeding and genetical, physiological or biochemical research, consequently it is an extremely dynamic field. Transgenic plants are commonly used to complete or substitute mutants in basic research, helping the studies of complex biological situations such as pathogenesis process, genome organization, light reception and signal transduction. In this review, recent approaches for foreign gene introduction (e.g. Agrobiolistics, whole tissue electroporation, in planta Agrobacterium transformation), screening (reporter gene possibilities and performance) and transformant selection (ipt selective marker) are discussed. Transgene expression and mechanisms underlying (trans)gene inactivation are presented.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant transformation; Agrobiolistics; Electroporation; Reporter genes; Transgenic crops. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000100001 |
| |
|
|
| Borges,R.M.; Horne,J.H.; Melo,A.; Vidal,J.T.; Vieceli,F.M.; Melo,M.O.; Kanno,T.Y.N.; Fraser,S.E.; Yan,C.Y.I.. |
| One of the challenges of the postgenomic era is characterizing the function and regulation of specific genes. For various reasons, the early chick embryo can easily be adopted as an in vivo assay of gene function and regulation. The embryos are robust, accessible, easily manipulated, and maintained in the laboratory. Genomic resources centered on vertebrate organisms increase daily. As a consequence of optimization of gene transfer protocols by electroporation, the chick embryo will probably become increasingly popular for reverse genetic analysis. The challenge of establishing chick embryonic electroporation might seem insurmountable to those who are unfamiliar with experimental embryological methods. To minimize the cost, time, and effort required to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Electroporation; In vivo gene expression; Electroporator; Vertebrate embryo. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900752 |
| |
|
| |
|
|
|
