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	Provedor de dados Genet. Mol. Biol. (2) BABT (1) Autor Chen,Xiao (1) Chen,Yingying (1) Cheng,De (1) Freitas,Erika Regina Leal de (1) Gambarini,Maria Lúcia (1) Gao,Xing (1) Guillo,Lidia Andreu (1) Huang,Qiong (1) Li,Yanwei (1) Li,Zhenzhen (1) Liu,Yajun (1) Oliveira Filho,Benedito Dias (1) Sanches,Bruno Valente (1) Song,Xinghui (1) Wang,Huayan (1) Wang,Linlin (1) Xu,Guowei (1) Yin,Guoli (1) Mais... Palavra-chave Embryonic stem cells (3) Adipocyte differentiation (1) Bovine blastocysts (1) DNA microarray analysis (1) Fibroblast growth factor (1) Gene expression profiling (1) Gene markers (1) ICM isolation (1) Induced pluripotent stem cells (1) Mesenchymal stem cells (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2014 (1) 2012 (1) 2011 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última The gene expression profiles of induced pluripotent stem cells (iPSCs) generated by a non-integrating method are more similar to embryonic stem cells than those of iPSCs generated by an integrating method Genet. Mol. Biol. Liu,Yajun; Cheng,De; Li,Zhenzhen; Gao,Xing; Wang,Huayan. Induced pluripotent stem cells (iPSCs) obtained by the ectopic expression of defined transcription factors have tremendous promise and therapeutic potential for regenerative medicine. Many studies have highlighted important differences between iPSCs and embryonic stem cells (ESCs). In this work, we used meta-analysis to compare the global transcriptional profiles of human iPSCs from various cellular origins and induced by different methods. The induction strategy affected the quality of iPSCs in terms of transcriptional signatures. The iPSCs generated by non-integrating methods were closer to ESCs in terms of transcriptional distance than iPSCs generated by integrating methods. Several pathways that could be potentially useful for studying the molecular... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA microarray analysis; Embryonic stem cells; Gene expression profiling; Induced pluripotent stem cells. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400022 bFGF promotes adipocyte differentiation in human mesenchymal stem cells derived from embryonic stem cells Genet. Mol. Biol. Song,Xinghui; Li,Yanwei; Chen,Xiao; Yin,Guoli; Huang,Qiong; Chen,Yingying; Xu,Guowei; Wang,Linlin. In this work we describe the establishment of mesenchymal stem cells (MSCs) derived from embryonic stem cells (ESCs) and the role of bFGF in adipocyte differentiation. The totipotency of ESCs and MSCs was assessed by immunofluorescence staining and RT-PCR of totipotency factors. MSCs were successfully used to induce osteoblasts, chondrocytes and adipocytes. MSCs that differentiated into adipocytes were stimulated with and without bFGF. The OD/DNA (optical density/content of total DNA) and expression levels of the specific adipocyte genes PPARγ2 (peroxisome proliferator activated receptor γ2) and C/EBPs were higher in bFGF cells. Embryonic bodies had a higher adipocyte level compared with cells cultured in plates. These findings indicate that bFGF promotes... Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryonic stem cells; Mesenchymal stem cells; Fibroblast growth factor; Adipocyte differentiation. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100019 Embryonic stem-like cells derived from in vitro produced bovine blastocysts BABT Freitas,Erika Regina Leal de; Sanches,Bruno Valente; Gambarini,Maria Lúcia; Oliveira Filho,Benedito Dias; Guillo,Lidia Andreu. The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine blastocysts; ICM isolation; Embryonic stem cells; Gene markers. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300009 Registros recuperados: 3 Primeira ... 1 ... Última

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