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	Provedor de dados BJMBR (6) Biol. Res. (2) BABT (1) Autor Beaudet,A. (1) Cai,Yingying (1) Chen,Pingsheng (1) Cruz-Amaya,Mirna (1) Dal-Farra,C. (1) Dominguez,W.V. (1) Ferguson,S.S.G. (1) Gomes,Elisa Maines (1) Gomez,M.V. (1) Guatimosim,C. (1) Han,S.W. (1) Hong,Tingting (1) Kanashiro,C.A. (1) Kong,Yanping (1) Krieger,J.E. (1) Liu,Lei (1) Manríquez,Nicolás (1) Merjan,A.J. (1) Nicastri,A.L. (1) Nicastri,Ana Lucia (1) Mais... Palavra-chave Endocytosis (9) Agonist-induced receptor internalization (1) Angiotensin II (1) Arrestin (1) Autophagy (1) Bence-Jones (1) Bence-Jones proteins (1) Cancer cells (1) Confocal microscopy (1) Crispr/cas9 (1) DNA uptake (1) Exocytosis (1) Experimental Nephrology (1) FM1-43 (1) Fluorescence-tagged receptor (1) G protein-coupled receptor kinases (1) G protein-coupled receptors (1) Green fluorescent protein (1) Internalization (1) Lambda light chain protein (1) Mais... Tipo do documento Info:eu-repo/semantics/article (6) Journal article (2) Info:eu-repo/semantics/other (1) Ano 2020 (1) 2017 (1) 2015 (1) 2002 (1) 2001 (2) 1998 (3) Mais... País Brazil (7) Chile (2) Idioma Inglês (9)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 9 Primeira ... 1 ... Última Uptake of DNA by cancer cells without a transfection reagent Biol. Res. Kong,Yanping; Zhang,Xianbo; Zhao,Yongliang; Xue,Yanfang; Zhang,Ye. Abstract Background Cancer cells exhibit elevated levels of glucose uptake and may obtain pre-formed, diet-derived fatty acids from the bloodstream to boost their rapid growth; they may also use nucleic acid from their microenvironment. The study of processing nucleic acid by cancer cells will help improve the understanding of the metabolism of cancer. DNA is commonly packaged into a viral or lipid particle to be transferred into cells; this process is called transfection in laboratory. Cancer cells are known for having gene mutations and the evolving ability of endocytosis. Their uptake of DNAs might be different from normal cells; they may take in DNAs directly from the environment. In this report, we studied the uptake of DNAs in cancer cells without... Tipo: Journal article Palavras-chave: DNA uptake; Cancer cells; Endocytosis. Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100205 Sortin2 enhances endocytic trafficking towards the vacuole in Saccharomyces cerevisiae Biol. Res. Vásquez-Soto,Beatriz; Manríquez,Nicolás; Cruz-Amaya,Mirna; Zouhar,Jan; Raikhel,Natasha V; Norambuena,Lorena. BACKGROUND: A highly regulated trafficking of cargo vesicles in eukaryotes performs protein delivery to a variety of cellular compartments of endomembrane system. The two main routes, the secretory and the endocytic pathways have pivotal functions in uni- and multi-cellular organisms. Protein delivery and targeting includes cargo recognition, vesicle formation and fusion. Developing new tools to modulate protein trafficking allows better understanding the endomembrane system mechanisms and their regulation. The compound Sortin2 has been described as a protein trafficking modulator affecting targeting of the vacuolar protein carboxypeptidase Y (CPY), triggering its secretion in Saccharomyces cerevisiae. RESULTS: A reverse chemical-genetics approach was used... Tipo: Journal article Palavras-chave: Endocytosis; Sortin2; Trafficking modulator; Saccharomyces cerevisiae. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100039 Nephrotoxicity of Bence-Jones proteins: correlation with endocytosis by BHK cells and intracellular movement BABT Nicastri,Ana Lucia; Gomes,Elisa Maines; Prado,Maria José Brandão de Almeida; Prado,Euthymia Brandão de Almeida. The aim of this investigation was to evaluate the endocytosis of two Bence-Jones proteins by renal cells in order to elucidate the interference of their physical and chemical characteristics on nephrotoxicity. Bence-Jones proteins (AK and GL) were purified and isolated from the urine of two patients with multiple myeloma. The isotype of both proteins was characterised as being human monoclonal lambda light chain. The AK protein presented mainly an Ip>7.0, a high content of galactose and a low amount of sialic acid molecules. On the other hand, the GL protein presented a single band with an Ip of 4.3, a higher level of sialic acid and a reduced amount of galactose, in comparison with the AK protein. Baby Hamster Kidney (BHK) cells were maintained in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Endocytosis; Bence-Jones; Experimental Nephrology. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200009 Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells BJMBR Merjan,A.J.; Kanashiro,C.A.; Krieger,J.E.; Han,S.W.; Paiva,A.C.M.. A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization. Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II; Agonist-induced receptor internalization; Endocytosis; Fluorescence-tagged receptor; Nuclear angiotensin receptors; Renin-angiotensin system. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011 Fluorescent ligands for studying neuropeptide receptors by confocal microscopy BJMBR Beaudet,A.; Nouel,D.; Stroh,T.; Vandenbulcke,F.; Dal-Farra,C.; Vincent,J.-P.. This paper reviews the use of confocal microscopy as it pertains to the identification of G-protein coupled receptors and the study of their dynamic properties in cell cultures and in mammalian brain following their tagging with specific fluorescent ligands. Principles that should guide the choice of suitable ligands and fluorophores are discussed. Examples are provided from the work carried out in the authors' laboratory using custom synthetized fluoresceinylated or BODIPY-tagged bioactive peptides. The results show that confocal microscopic detection of specifically bound fluorescent ligands permits high resolution appraisal of neuropeptide receptor distribution both in cell culture and in brain sections. Within the framework of time course experiments,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Neurotensin; Somatostatin; Opioids; Internalization; Endocytosis; Confocal microscopy. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100017 Use of fluorescent probes to follow membrane traffic in nerve terminals BJMBR Guatimosim,C.; Romano-Silva,M.A.; Gomez,M.V.; Prado,M.A.M.. Optical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have been gained by visualization of vesicles with the vital dye FM1-43. A number of questions related to the control of recycling of synaptic vesicles by prolonged stimulation and the role of calcium to control membrane internalization are now being addressed. It is expected that optical monitoring of presynaptic activity coupled to appropriate genetic... Tipo: Info:eu-repo/semantics/article Palavras-chave: Laser scanning confocal microscopy; FM1-43; Exocytosis; Synaptic vesicles; Endocytosis. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100018 Nephrotoxicity of Bence-Jones proteins: interference in renal epithelial cell acidification BJMBR Nicastri,A.L.; Prado,M.J.B.A.; Dominguez,W.V.; Prado,E.B.A.. The aim of the present study was to evaluate the acidification of the endosome-lysosome system of renal epithelial cells after endocytosis of two human immunoglobulin lambda light chains (Bence-Jones proteins, BJP) obtained from patients with multiple myeloma. Renal epithelial cell handling of two BJP (neutral and acidic BJP) was evaluated by rhodamine fluorescence. Renal cells (MDCK) were maintained in culture and, when confluent, were incubated with rhodamine-labeled BJP for different periods of time. Photos were obtained with a fluorescence microscope (Axiolab-Zeiss). Labeling density was determined on slides with a densitometer (Shimadzu Dual-Wavelength Flying-Spot Scanner CS9000). Endocytosis of neutral and acidic BJP was correlated with acidic... Tipo: Info:eu-repo/semantics/other Palavras-chave: Bence-Jones proteins; Renal cell acidification; Endocytosis; Lambda light chain protein. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300011 Effects of Rab7 gene up-regulation on renal fibrosis induced by unilateral ureteral obstruction BJMBR Xu,Qing; Liu,Lei; Yang,Yiqiong; Wang,Zhi; Cai,Yingying; Hong,Tingting; Chen,Pingsheng. Rab7, an important member of the Rab family, is closely related to autophagy, endocytosis, apoptosis, and tumor suppression but few studies have described its association with renal fibrosis. In the early stage, our group studied the effects of Rab7 on production and degradation of extracellular matrix in hypoxic renal tubular epithelial cells. Because cell culture in vitro is different from the environment in vivo, it is urgent to understand the effects in vivo. In our current study, we established a renal fibrosis model in Rab7-knock-in mice (prepared by CRISPR/Cas9 technology) and wild type (WT) C57BL/6 mice using unilateral ureteral obstruction (UUO). Seven and 14 days after UUO, the expression of the Rab7 protein in WT mice, as well as the autophagic... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rab7; Autophagy; Renal fibrosis; Crispr/cas9; Endocytosis; Lipophagy. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400603 Using green fluorescent protein to understand the mechanisms of G-protein-coupled receptor regulation BJMBR Ferguson,S.S.G.. G protein-coupled receptor (GPCR) activation is followed rapidly by adaptive changes that serve to diminish the responsiveness of a cell to further stimulation. This process, termed desensitization, is the consequence of receptor phosphorylation, arrestin binding, sequestration and down-regulation. GPCR phosphorylation is initiated within seconds to minutes of receptor activation and is mediated by both second messenger-dependent protein kinases and receptor-specific G protein-coupled receptor kinases (GRKs). Desensitization in response to GRK-mediated phosphorylation involves the binding of arrestin proteins that serve to sterically uncouple the receptor from its G protein. GPCR sequestration, the endocytosis of receptors to endosomes, not only... Tipo: Info:eu-repo/semantics/article Palavras-chave: Arrestin; Endocytosis; Green fluorescent protein; G protein-coupled receptors; G protein-coupled receptor kinases; Resensitization. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100016 Registros recuperados: 9 Primeira ... 1 ... Última

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