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	Provedor de dados 5 (3) Autor Le Guyader, Soizick (3) Dubois, Eric (2) Haugarreau, Larissa (2) Miossec, Laurence (2) Pommepuy, Monique (2) Atmar, Robert (1) Estes, Mary (1) Neill, Frederick (1) Schwab, Kellogg (1) Mais... Palavra-chave Enteric virus (3) Human pathology (2) Molecular technique (2) Seasonal variation (2) Shellfish (2) Viral contamination (2) Bivalvia (1) DNA enzyme (1) Detection method (1) Immuoessay (1) Reverse transcription PCR (1) Mais... Tipo do documento Text (3) Ano 2001 (1) 2000 (2) País France (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Development of a reverse transcription-PCR-DNA enzyme immunoassay for detection of "Norwalk-like" viruses and hepatitis A virus in stool and shellfish 5 Schwab, Kellogg; Neill, Frederick; Le Guyader, Soizick; Estes, Mary; Atmar, Robert. Outbreaks of food- and waterborne gastroenteritis are being increasingly reported throughout the world. The analysis of environmental samples by ne,ver diagnostic techniques such as reverse transcription-PCR (RT-PCR) amplification of nucleic acid has begun to identify human enteric viruses (predominantly "Norwalklike" viruses [NLVs]) as the cause of many of these outbreaks. To streamline NLV detection from environmental samples such as shellfish, we have developed an RT-PCR-oligoprobe amplification and detection method using several new procedures that enable confirmed RT-PCR amplification and product detection in 1 day. The new steps include replacing reverse transcriptase and Tag polymerase with rTth polymerase, a heat-stable enzyme that functions as... Tipo: Text Palavras-chave: Bivalvia; Immuoessay; DNA enzyme; Reverse transcription PCR; Detection method; Enteric virus. Ano: 2001 URL: https://archimer.ifremer.fr/doc/2001/publication-1267.pdf Three-year study to assess human enteric viruses in shellfish 5 Le Guyader, Soizick; Haugarreau, Larissa; Miossec, Laurence; Dubois, Eric; Pommepuy, Monique. The main pathogenic enteric viruses able to persist in the environment, such as hepatitis A virus (HAV), Norwalk-like virus (NLV), enterovirus (EV), rotavirus (RV), and astrovirus (AV), were detected by reverse transcription-PCR and hybridization in shellfish during a 3-year study. Oyster samples (n = 108), occasionally containing bacteria, were less frequently contaminated, showing positivity for AV (17%), NLV (23%), EV (19%), and RV (27%), whereas mussel samples, collected in areas routinely impacted by human sewage, were more highly contaminated: AV (50%), HAV (13%), NLV (35%), EV (45%), and RV (52%). Sequences obtained from HAV and NLV amplicons shelved a great variety of strains, especially for NLV (strains close to Mexico, Snow Mountain Agent, or... Tipo: Text Palavras-chave: Molecular technique; Seasonal variation; Human pathology; Viral contamination; Shellfish; Enteric virus. Ano: 2000 URL: https://archimer.ifremer.fr/doc/00507/61879/65953.pdf Three-year study to assess human enteric viruses in shellfish 5 Le Guyader, Soizick; Haugarreau, Larissa; Miossec, Laurence; Dubois, Eric; Pommepuy, Monique. The main pathogenic enteric viruses able to persist in the environment, such as hepatitis A virus (HAV), Norwalk-like virus (NLV), enterovirus (EV), rotavirus (RV), and astrovirus (AV), were detected by reverse transcription-PCR and hybridization in shellfish during a 3-year study. Oyster samples (n = 108), occasionally containing bacteria, were less frequently contaminated, showing positivity for AV (17%), NLV (23%), EV (19%), and RV (27%), whereas mussel samples, collected in areas routinely impacted by human sewage, were more highly contaminated: AV (50%), HAV (13%), NLV (35%), EV (45%), and RV (52%). Sequences obtained from HAV and NLV amplicons shelved a great variety of strains, especially for NLV (strains close to Mexico, Snow Mountain Agent, or... Tipo: Text Palavras-chave: Molecular technique; Seasonal variation; Human pathology; Viral contamination; Shellfish; Enteric virus. Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-1363.pdf Registros recuperados: 3 Primeira ... 1 ... Última

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