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Registros recuperados: 9
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Possible relation between a winter epidemic of acute gastroenteritis in France and viral contamination of shellfish ArchiMer
Miossec, Laurence; Le Guyader, Soizick; Haugarreau, Larissa; Comps, Marie-annick; Pommepuy, Monique.
Several outbreaks of gastroenteritis related to the consumption of shellfish (frequently eaten raw) have been reported in different parts of the world. In Europe, hum an calicivirus infections may have been involved in winter outbreaks in recent years, although there is little evidence confirming such viral contamination in shellfish. This study presents the first results of a field survey on viral contamination in two shelllish harvesting areas along the French Mediterranean coast. The first, consisting mainly of oyster beds, was c1assified in category A, as determined by fecal coliform counts in shellfish (European Community Directive 91/492), and the second, a mussel bed, was c1assified in category C. Shelllish samples were collected monthly between...
Tipo: Text Palavras-chave: Shellfish; Viral contamination; Enterovirus; Human calicivirus; Rotavirus; Astrovirus; Fecal coliforms; Gastroenteritis.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/00001/11257/7802.pdf
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Development of genomic probes for detection of hepatitis A virus and enteroviruses in shellfish ArchiMer
Apaire, M; Leguyader, F; Besse, B; Billaudel, S.
The development of molecular biology techniques has modified research procedures for certain viruses, particularly hepatitis A virus (HAV) and enteroviruses. Accordingly, we designed cDNA and RNA genomic probes to detect viruses in shellfish in the environment. Better sensitivity was noted with RNA than cDNA probes: only 17/83 shellfish samples were positive with HAV and enterovirus DNA probes, where as 42/83 were positive with HAV and enterovirus RNA probes.
Tipo: Text Palavras-chave: Mollusca; Enterovirus; Hepatitis A virus; Picornaviridae; RNA probes; DNA probes; Seafood; Biology; Sensors; DNA; RNA; Viruses; Detection; Marine molluscs; Shellfish.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1613.pdf
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Viral contamination and depuration of oysters under field conditions ArchiMer
Boher, S; Piclet, Guy; Beril-stien, S; Masson, Daniel; Delonce, R; Biziagos, E; Schwartzbrod, L.
Shellfish consumption is clearly associated with the transmission of enteric viruses. The channel of transmission of enteric viruses to human beings could be decreased particularly if the systems of depuration of shellfish were optimized, standardized and if their viral efficiency was controlled. The purpose of this work was to study the contamination of oysters and to assess the efficiency of several depuration processes. Our study have demonstrated that in some cases, 50% of oysters samples contain enteric viruses or H.A.V. antigen and the quantities of viruses range from 17 to 246 FF for the rotaviruses and from 124 to 200 for the enteroviruses per 100 g of tissue. According to our results, the exclusive use of a bacteriological criterium to determine...
Tipo: Text Palavras-chave: Rotavirus; Hepatitis A virus; Enterovirus; Crassostrea gigas; Bivalvia; Seafood; Bioassays; Cell culture; Viruses; Self purification; Viral diseases; Shellfish.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1615.pdf
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Diversity of enterovirus sequences detected in oysters by RT-heminested PCR ArchiMer
Dubois, Eric; Merle, Ghislaine; Roquier, Catherine; Trompette, Aurélien; Le Guyader, Soizick; Cruciere, Catherine; Chomel, Jean-jacques.
Oysters harvested in western France, from five sites associated with outbreaks of food-borne norovirus gastroenteritis between February 2000 and March 2001, were assayed for enterovirus RNA by reverse transcriptase-heminested polymerase chain reaction (RT-heminested PCR). Forty percent (21/52) of shellfish samples (pool of seven oysters) were contaminated by enteroviruses. Infectious coxsackieviruses serotype A21 were isolated from three of these positive samples. Amplicons corresponding to 65 base sequences in the 5' untranslated region of the enteroviral genome were analyzed by direct sequencing. Interpretable results were obtained from 18 amplicons, but mixtures of sequences confused the results from 3 samples. Sequences isolated from samples from the...
Tipo: Text Palavras-chave: Sequence analyze; RT PCR; Cell culture; Shellfish; Enterovirus.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-763.pdf
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Enterovirus infections and type 1 diabetes mellitus: is there any relationship? J. Venom. Anim. Toxins incl. Trop. Dis.
Tavares,RG; Trevisol,RB; Comerlato,J; Dalzochio,T; Feksa,LR; Spilki,FR; Berlese,DB.
Several health organizations have classified diabetes mellitus, a metabolic syndrome, as the epidemic of the century, since it affects millions of people worldwide and is one of the top ten causes of death. Type 1 diabetes is considered to be an autoimmune disease, in which autoaggressive T cells infiltrate the islets of Langerhans in the pancreas, leading to the destruction of insulin producing beta cells. The risk of the disease is modulated by genetic factors, mainly genes coding for human leukocyte antigens (HLA). However, the incidence of this disease has increased significantly during the recent decades, which cannot be explained only by genetic factors. Environmental perturbations have also been associated to the development of diabetes. Among these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diabetes mellitus type I; Enterovirus; Autoimmune disease.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100002
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Description of a widespread outbreak of aseptic meningitis due to echovirus 30 in Rio de Janeiro state, Brazil BJID
Pinto Junior,Vitor Laerte; Rebelo,Maria Cristina; Costa,Eliane Veiga da; Silva,Edson Elias da; Bóia,Márcio Neves.
Echovirus 30 belongs to the genus Enterovirus and is widely associated with aseptic meningitis (AM) outbreaks. In Brazil epidemics due to this serotype were reported in several states but in Rio de Janeiro, before this study, it was only involved in sporadic episodes. We retrospectively collected data from AM notifications charts and enterovirus isolation database from Rio de Janeiro State Health Department (RJSHD) and Enterovirus Reference Laboratory in the year of 2005. An outbreak of AM was detected during March, April and May associated with a high cell culture isolation rate for echovirus 30 (17.4%). Male children with ages varying from 1 to 9 years were more affected. Of the 22 patients with confirmed echovirus 30 disease, clinical information was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aseptic meningitis; Enterovirus; Echoviruses; Cerebrospinal fluid.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000500011
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The role of microRNAs in enteroviral infections BJID
Wu,Jing; Shen,Li; Chen,Jianguo; Xu,Huaxi; Mao,Lingxiang.
ABSTRACTThe genus Enterovirus, a member of thePicornavirus family, are RNA viruses that can cause poliomyelitis, hand-food-mouth disease, viral meningitis or meningoencephalitis, viral myocarditis and so on. MicroRNAs are a class of highly conserved, small noncoding RNAs recognized as important regulators of gene expression. Recent studies found that MicroRNAs play a significant role in the infection ofEnterovirus, such as enterovirus 71, coxsackievirus B3 and other Enterovirus. Enteroviral infection can alter the expression of cellular MicroRNAs, and cellular MicroRNAs can modulate viral pathogenesis and replication by regulating the expression level of viral or host's genes. Herein, this review summarizes the role of MicroRNAs in enteroviral infection.
Tipo: Info:eu-repo/semantics/article Palavras-chave: MicroRNAs; Enterovirus; Infection.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500510
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Rhinovirus detection using different PCR-based strategies BJM
Silva,E.R.M.; Watanabe,A.S.A.; Carraro,E.; Perosa,A.H.S.; Granato,C.F.H.; Bellei,N.C.J..
Human rhinoviruses (HRVs) are the major cause of the common cold. HRVs were recently reclassified into the Enterovirus genus (HEV) in the Picornaviridae family. HRVs and other members of the HEV genus share many common features, including sense RNA genomes and partial nucleotide sequence identity. The aim of this study was to evaluate different HRV detection strategies. Samples from adults with acute respiratory infection (n = 291) who were treated in Sao Paulo Hospital (2001-2003) were tested using three assays. The first assay detected picornaviruses by RT-PCR and hybridization, the second detected rhinoviruses using RT-PCR/sequencing, and the third differentiated HRV from HEV using duplex semi-nested-RT-PCR. Analysis of the results obtained from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhinovirus; Enterovirus; Detection; Differentiation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200038
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Aseptic meningitis by echovirus 30 in São Paulo state, Brazil BJM
Machado,Bráulio Caetano; Ferreira,Raquel de Souza; Carmona,Rita de Cássia Compagnoli; Timenetsky,Maria do Carmo Sampaio Tavares.
The objective of this study was to identify and characterize the immunological and molecular aspects of Human Enterovirus (HEV) associated with aseptic meningitis cases in São Paulo State, Brazil. Clinical samples (cerebrospinal fluid CSF, stool and sera) were analyzed from 21 viral meningitis suspected cases collected in the period from 1998 to 1999. The isolated virus were identified as belonging to Enterovirus genus and specifically to echovirus serotype 30 (EV-30), using immunological techniques as Indirect Immunofluorescense Assay (IFA) and Neutralization (Nt) and by molecular technique of Reverse Transcription Polymerase Chain Reaction (RT-PCR) directed to the 5' NTR and VP1 regions of viral genome. The results obtained contributed with to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterovirus; Viral meningitis; Molecular and immunological diagnosis; RT-PCR for Enterovirus; Indirect imunnofluorescense assay.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100020
Registros recuperados: 9
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