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| SCHREIER,STEFAN; TRIAMPO,WANNAPONG; DOUNGCHAWEE,GALAYANEE; TRIAMPO,DARAPOND; CHADSUTHI,SUDARAT. |
| Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density and comparing this to automated direct counting using a counting chamber in combination with imaging and analyzing software. Two serovars, Bangkok and Copenhagenii, from log-phase growth were used for the establishment of standard curves. They were found to be linear and slightly different in gradient for each serovar. The ease, rapidity, and reliability of these two adapted and optimized counting techniques may provide a useful alternative enumeration technique... |
| Tipo: Journal article |
Palavras-chave: Leptospirosis; Leptospira; Enumeration; Spectroscopy; Indirect counting. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100001 |
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| Machado,SCA; Pereira,VLA; Aquino,MHC; Giombeli,A; Rodrigues,DP; Nascimento,ER. |
| ABSTRACT The present study investigated the frequency, level of contamination and serotyping of Salmonella strains isolated from broiler flocks in different processing sites and the fulfillment of a Performance Objective (PO) in frozen chicken breasts, as a risk assessment to measure the efficacy of prevention and control programs applied to reduce the risk of Salmonella spp. in raw poultry meat that contribute to reach food safety and public health goals. From 1,800 samples of cloacal swabs, carcasses before and after immersion chilling and frozen breasts derived from 20 broiler flocks slaughtered at two processing plants located in the mid-west and southern regions of Brazil, 278 samples were positive for Salmonella spp. by polymerase chain reaction... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler; Chicken meat; Salmonella; Serotype; Enumeration; Performance objective (PO). |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100328 |
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