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	Provedor de dados Ciênc. Tecnol. Aliment. (1) Rev. Bras. Ciênc. Solo (1) Autor GUT,Jorge Andrey Wilhelms (1) Lavres Junior,José (1) Monteiro,Francisco Antonio (1) SIGUEMOTO,Érica Sayuri (1) Santos Junior,João de Deus Gomes dos (1) Palavra-chave Enzymatic assay (2) Apple juice (1) Chlorophyll (1) Grass forage (1) Microplate (1) Nutritional status (1) Peroxidase (1) Polyphenol oxidase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2017 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Validation of spectrophotometric microplate methods for polyphenol oxidase and peroxidase activities analysis in fruits and vegetables Ciênc. Tecnol. Aliment. SIGUEMOTO,Érica Sayuri; GUT,Jorge Andrey Wilhelms. Abstract Enzymes polyphenol oxidase (PPO) and peroxidase (POD) play important roles in the processing of fruits and vegetables, since they can produce undesirable changes in color, texture and flavor. Classical methods of activity assessment are based on cuvette spectrophotometric readings. This work aims to propose, to validate and to test microplate spectrophotometric methods. Samples of apple juice and lyophilized enzymes from mushroom and horseradish were analyzed by the cuvette and microplate methods and it was possible to validate the microplate assays with satisfactory results regarding linearity, repeatability, accuracy along with quantitation and detection limits. The proposed microplate methods proved to be reliable and reproducible as the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Microplate; Polyphenol oxidase; Peroxidase; Enzymatic assay; Apple juice. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000500148 Nitrate reductase activity and spad readings in leaf tissues of guinea grass submitted to nitrogen and potassium rates Rev. Bras. Ciênc. Solo Lavres Junior,José; Santos Junior,João de Deus Gomes dos; Monteiro,Francisco Antonio. Nitrogen and K deficiency are among the most yield limiting factors in Brazilian pastures. The lack of these nutrients can hamper the chlorophyll biosynthesis and N content in plant tissues. A greenhouse experiment was carried out to evaluate the relationship among N and K concentrations, the indirect determination of chlorophyll content (SPAD readings), nitrate reductase activity (RNO3-) in newly expanded leaf lamina (NL) and the dry matter yield for plant tops of Mombaça grass (Panicum maximum Jacq.). A fractionated 5² factorial design was used, with 13 combinations of N and K rates in the nutrient solution. The experimental units were arranged in a randomized block design, with four replications. Plants were harvested twice. The first harvest occurred... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorophyll; Enzymatic assay; Nutritional status; Grass forage. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000300022 Registros recuperados: 2 Primeira ... 1 ... Última

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