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Interference of a commercial catalase preparation in laccase and peroxidase activities BABT
Ballaminut,Nara; Yamanaka,Renata; Machado,Katia Maria Gomes.
The influence of commercial catalase preparations (fungal and bovine origin) on laccase and peroxidase activity assays was evaluated using enzymatic extracts obtained from several basidiomycetes grown under different culture conditions. No hydrogen peroxide was detected in the extracts. Inhibition of laccase activity by 40 to 80% was related to the catalase source. In addition, oxidation of the substrate (ABTS) by fungal catalase in the absence of the enzymatic extract from basidiomycetes was observed. The results demonstrated the need for the evaluation of interference of the commercial catalase preparation when its use was required in the reaction mixture.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxidase; Fungi; Basidiomycetes; Hydrogen peroxide; Enzymatic inhibition.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500017
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Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage BJM
Lima e Silva,Agostinho A. de; Carvalho,Márcia A. Ribeiro de; Souza,Sérgio A. L de; Dias,Patrícia M. Teixeira; Silva Filho,Renato G. da; Saramago,Carmen S. de Meirelles; Bento,Cleonice A. de Melo; Hofer,Ernesto.
Samples of sewage from a university hospital and a chemistry technical school were analysed for the percentage of bacterial tolerance to chromium (Cr), silver (Ag) and mercury (Hg). Additionally, we investigated the effect of these metals on pigmentation and on some enzymatic activities of the metal tolerant strains isolated, as well as antimicrobial resistance in some metal tolerant Enterobacteriaceae strains. Tolerance to Cr was observed mainly in Gram positive bacteria while in the case of Ag and Hg the tolerant bacteria were predominately Gram negative. Hg was the metal for which the percentage of tolerance was significantly higher, especially in samples from the hospital sewage (4.1%). Mercury also had the most discernible effect on color of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metal tolerance; Sewage; Pigment; Enzymatic inhibition; Antimicrobial resistance.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400047
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