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| Registros recuperados: 39 | |
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| Silva, Laércio Junio da; Dias, Denise Cunha Fernandes dos Santos; Sekita, Marcelo Coelho; Finger, Fernando Luiz. |
| The objective of the present work was to investigate the antioxidant enzyme activities and the occurrence of lipid peroxidation during storage of J. curcas seeds obtained from fruits at different maturity stages. Seeds extracted from fruits collected at three stages of maturity were used, i.e., yellow, brownish-yellow, and brown (dry fruits). The seeds were subsequently stored for 18 months in Kraft paper bags in a laboratory environment. Initially as well as every three months, the seeds were evaluated for germination and electrical conductivity, and the seed embryos were evaluated for lipid peroxidation; protein content; and superoxide dismutase, peroxidase, and ascorbate peroxidase enzyme activity at the same time points. In general, the seeds from the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.01.03.03-2 physic nut; Seed maturation and deterioration; Enzyme activity; Malondialdehyde content. Produção e Beneficiamento de Sementes. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/34978 |
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| Silva, Laércio Junio da; Dias, Denise Cunha Fernandes dos Santos; Sekita, Marcelo Coelho; Finger, Fernando Luiz. |
| The objective of the present work was to investigate the antioxidant enzyme activities and the occurrence of lipid peroxidation during storage of J. curcas seeds obtained from fruits at different maturity stages. Seeds extracted from fruits collected at three stages of maturity were used, i.e., yellow, brownish-yellow, and brown (dry fruits). The seeds were subsequently stored for 18 months in Kraft paper bags in a laboratory environment. Initially as well as every three months, the seeds were evaluated for germination and electrical conductivity, and the seed embryos were evaluated for lipid peroxidation; protein content; and superoxide dismutase, peroxidase, and ascorbate peroxidase enzyme activity at the same time points. In general, the seeds from the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Physic nut; Seed maturation and deterioration; Enzyme activity; Malondialdehyde content.; Produção e Beneficiamento de Sementes. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/34978 |
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| Rocha, Denise Alvarenga; Queiroz, Estela de Rezende; Botelho, Lucimara Nazaré Silva; Fráguas, Rodrigo Martins; Santos, Cláudia Mendes dos; Abreu, Celeste Maria Patto; Sousa, Raimundo Vicente de. |
| The pineapple is a fruit that has wide acceptance worldwide both in natural form, as industrialized. Your peel is a residue generated by food industries and from this residue can obtain a protein extract which is a good source of bromelain. This study aimed to obtain a protein extract from pineapple peel, evaluate its enzyme activity and its healing properties in skin lesions in rats. Seven animal groups were used: control, treated with 5% of protein extract, 10% of protein extract and pure protein extract; 5% of commercial bromelain, 10% of commercial bromelain and pure commercial bromelain. The animals were subjected to a tissue incision and treated for 21 days. Proteolytic and specific activities of the protein extract were 1.30 U mg-1 and 45 x 10-3 U... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ciências Biológicas bromelain; Skin lesion; Enzyme activity; Pineapple and healing. Bioquímica. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/41466 |
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| PEIL,GREICE H.S.; KUSS,ANELISE V.; RAVE,ANDRÉS F.G.; VILLARREAL,JOSÉ P.V.; HERNANDES,YOHANA M.L.; NASCENTE,PATRÍCIA S.. |
| ABSTRACT The lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. The aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in Pelotas, RS/Brazil. Bacteria were identified using Gram stain and biochemical tests (Vitek 2(r)). Fungi were identified according to macro and micromorphology characteristics. The extracellular lipase production was evaluated using the Rhodamine B test and the enzymatic activity by titration. Twenty-one bacteria were isolated and identified as Klebsiella pneumoniae ssp. pneumoniae, Serratia marcescens, Enterobacter aerogenes, Raoultella ornithinolytica and Raoultella planticola. Were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Effluent; Enzyme activity; Extracellular lipase production; Lipase enzyme; Lipolytic microorganism. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501769 |
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| Dorneles,Athos Odin Severo; Pereira,Aline Soares; Sasso,Victória Martini; Possebom,Gessieli; Tarouco,Camila Peligrinotti; Schorr,Márcio Renan Weber; Rossato,Liana; Ferreira,Paulo Ademar Avela; Tabaldi,Luciane Almeri. |
| ABSTRACT Potato cultivation is widespread around the world, being exposed to several abiotic stresses, including soils with high aluminum (Al) availability. Silicon (Si) is recognized for alleviating the stress caused by Al in various plant species. Thus, the aim of this study was to investigate the potential of Si to mitigate the oxidative stress caused by Al in potato genotypes, exhibiting differential sensitivity toward this element. Plants of the Al-sensitive genotype (SMIJ319-7) and Al-tolerant genotype (SMIF212-3) were grown for two weeks in a hydroponic system with the nutrient solution containing combinations of Al (0 and 1.85 mM) and Si (0, 0.5 and 1.0 mM). At the end of the experiment, photosynthetic parameters, pigment content, root and shoot... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; Beneficial elements; Enzyme activity; Oxidative stress; Toxic elements. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000100012 |
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| Silva,A.S.; Jacques,R.J.S.; Andreazza,R.; Bento,F.M.; Roesch,L.F.W.; Camargo,F.A.O.. |
| Polycyclic aromatic hydrocarbons (PAH) are carcinogenic compounds which contaminate water and soil, and the enzymes can be used for bioremediation of these environments. This study aimed to evaluate some environmental conditions that affect the production and activity of the catechol 1,2-dioxygenase (C12O) by Mycobacterium fortuitum in the cell free and immobilized extract in sodium alginate. The bacterium was grown in mineral medium and LB broth containing 250 mg L-1 of anthracene (PAH). The optimum conditions of pH (4.0-9.0), temperature (5-70 ºC), reaction time (10-90 min) and the effect of ions in the enzyme activity were determined. The Mycobacterium cultivated in LB shown higher growth and the C12O activity was two-fold higher to that in the mineral... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anthracene; Enzyme activity; Enzyme immobilization; Biodegradation; Waste treatment. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100043 |
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| Joshi,Gopal K.; Kumar,Sarvesh; Sharma,Vinay. |
| A moderately cold active, extracellular alkaline protease producing bacterium was isolated from a fresh water lake. The isolate was found to be a gram-positive, rod shaped organism later identified as Bacillus cereus MTCC 6840. The bacterium produced the maximum amount of enzyme when allowed to grow for 24 h at temperature 25º and pH 9.0. Among a variety of substrates used, fructose as a carbon source and a combination of yeast extract and peptone as nitrogen source, supported the maximum protease production by the organism (120 U/ml). Fe++ and Co++ stimulated the enzyme activity whereas Ca++, Cu++, K+, Mg++ and Mn++ inhibited it to different extents. The protease was found to be highly stable in the presence of NaCl, SDS and acetone. Treatment with EDTA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alkaline Protease; Bacillus cereus; Enzyme activity. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400034 |
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| Lisboa,Helen Cristina Fávero; Biasetto,Carolina Rabal; Medeiros,João Batista de; Araújo,Ângela Regina; Silva,Dulce Helena Siqueira; Teles,Helder Lopes; Trevisan,Henrique Celso. |
| A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project "Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest". The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi (EC. 3.1.1.1 -carboxylesterases) for production of carboxylic acids. Thus, the reduction of the pH increases... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Esterase; PH indicator; Enzyme activity; Endophytic fungi. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300042 |
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| BOSSO,Alessandra; MORIOKA,Luiz Rodrigo Ito; SANTOS,Leandro Freire dos; SUGUIMOTO,Hélio Hiroshi. |
| Abstract The commercial enzyme (E.C. = 3.2.1.23) from Kluyveromyces lactis (liquid) and Aspergillus oryzae(lyophilized) was investigated for its hydrolysis potential in lactose substrate, UHT milk, and skimmed milk at different concentrations (0.7; 1.0 and 1.5%), pH values (5.0; 6.0; 6.5 and 7.0), and temperature (30; 35; 40 and 55 ºC). High hydrolysis rates were observed for the enzyme from K. lactis at pH 7.0 and 40 ºC, and from A. oryzae at pH 5.0 and 55 ºC. The enzyme from K. lactis showed significantly higher hydrolysis rates when compared to A. oryzae. The effect of temperature and β-galactosidase concentration on the lactose hydrolysis in UHT milk was higher than in skimmed milk, for all temperatures tested. With respect to the thermal stability, a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: UHT and skimmed milk; Β-galactosidase; Enzymatic characterization; Lactose intolerance; Enzyme activity. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000100159 |
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| MACEDO,Adrielle Souza Leão; ROCHA,Fátima de Souza; RIBEIRO,Margareth da Silva; SOARES,Sergio Eduardo; BISPO,Eliete da Silva. |
| Abstract The reactions leading to the formation of precursors of chocolate flavor are performed by endogenous enzymes present in the cocoa seed. Polyphenol oxidase (PPO) presence and activity during fermentation of cocoa beans is responsible for the development of flavor precursors and is also implicated in the reduction of bitterness and astringency. However, the reliability of cocoa enzyme activities is complicated due to variations in different genotypes, geographical origins and methods of fermentation. In addition, there is still a lack of systematic studies comparing different cocoa cultivars. So, the present study was designed to characterize the activity of PPO in the pulp and seeds of two cocoa cultivars, PH 16 and TSH 1188. The PPO activity was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cocoa; Fermentation; Enzyme activity; Chocolate. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000100056 |
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| Shen,Yuwen; Lin,Haitao; Song,Xiaozong; Liu,Ping; Bo,Luji; Chen,Jianqiu; Yang,Li. |
| ABSTRACT: The influences of byproduct amendments, containing silicon, calcium, magnesium and potassium, on acidic soil quality in Jiaodong Peninsula of China had been studied and compared with that of lime through monitoring physicochemical properties and enzyme activities of acidic soil over a 120-day period. Byproduct amendments (1125, 2250, 4500 and 9000 kg ha-1) and lime 2250 kg ha-1 was applied in the acidic soil. Results showed that both byproduct amendments and lime significantly increased the pH, EC and enzyme activities of soil. The by-product amendments inhibited microbial biomass carbon and soil respiration. Nevertheless, the lime-treated soil had a much more higher level of CO2 emission than the by-product amendments-treated soil. Compared to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Soil amendments; Soil pH; Soil respiration; Enzyme activity. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600252 |
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| Oliveira,Tiago Ferreira Birro; Bertechini,Antônio Gilberto; Philomeno,Renato; Silva,Vanessa Avelar. |
| ABSTRACT: An experiment was conducted to study the effects of two sources and dietary levels of selenium (Se) on performance, Se concentration and glutathione peroxidase activity in plasma and liver of piglets post weaning. A total of 126 weaned pigs male and female Large White × Landrace, at average body weight of 7.2±0.67kg were allotted to seven treatments in a randomized block design, with two Se sources (organic and inorganic), inclusion levels, two levels for the inorganic source (0.30 and 0.60ppm) and four for the organic source (0.15, 0.30, 0.45 and 0.60ppm) and one control diet without Se supplementation containing 0.095ppm, and six replicates of three animals per pen. The experimental period was 35 days post-weaning. The organic source contained... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme activity; Glutathione peroxidase; Hepatic selenium; Plasmatic selenium. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200653 |
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| Registros recuperados: 39 | |
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