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	Provedor de dados Electron. J. Biotechnol. (2) Anais da ABC (AABC) (1) BJM (1) Rev. Microbiol. (1) BJPS (1) Rev. Bras. Frutic. (1) Mais... Autor Lima Filho,José Luiz de (2) Maia,Maria de Mascena Diniz (2) Morais,Marcia Maria Camargo de (2) BRITO,EDY SOUSA DE (1) CAMPOS,MARIA RITA C. (1) Chen,Wen-Bo (1) ESPÍNDOLA,SUÉLLEN P. (1) FERREIRA,ADÃO S. (1) González,José Miguel (1) Guan,Li-Ping (1) Gómez,José Manuel (1) Illanes,Andrés (1) Jin,Qing-Hao (1) Khan,Sana (1) Knight,Kirsty (1) Ledingham,William M. (1) Liu,Bing-Yu (1) MIRANDA,MARIA RAQUEL ALCÂNTARA DE (1) MOURA,CARLOS FARLEY HERBSTER (1) Melo,Eduardo Henrique Magalhães (1) Mais... Palavra-chave Enzyme kinetics (7) Anacardium occidentale (1) Annona muricata (1) Buffer (1) CDC25B (1) Cerrado (1) Dacron (1) Diffusional restrictions (1) Dioxoindolin-N-phenylacetamide (1) Eadie-Hofstee model (1) Enthalpy (1) Entropy (1) Ester hydrolysis (1) Extracellular lipase (1) Fermentation (1) Fusarium solani (1) Fusarium solani FS1 (1) Immobilized enzyme (1) Immobilized lipase (1) Induction (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Journal article (2) Info:eu-repo/semantics/other (1) Ano 2020 (1) 2016 (2) 2010 (1) 2005 (1) 2000 (1) 1999 (1) Mais... País Brazil (5) Chile (2) Idioma Inglês (7)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 7 Primeira ... 1 ... Última Hyper-production of a thermotolerant β-xylosidase by a deoxy-D-glucose and cycloheximide resistant mutant derivative of Kluyveromyces marxianus PPY 125 Electron. J. Biotechnol. Rajoka,Muhammad Ibrahim; Khan,Sana. Production of β-xylosidaseby a cycloheximide and 2-deoxy-D-glucose-resistant mutant of Kluyveromyces marxianus PPY125 was studied when cultured on growth media containing galactose, glucose, xylose, cellobiose, sucrose and lactose as carbon sources. Xylose, cellobiose, lactose and sucrose were the key substrates. Both K. marxianus PPY125 and its mutant (M 125) supported maximum β-xylosidase specific product yield (Y P/X) following growth on xylose. Basal level of activity was observed in non-induced cultures grown on glucose. The mutant produced 1.5 to 2-fold more β-xylosidase than that produced by the wild cells. Synthesis of β-xylosidase was regulated by an induction mechanism in both wild and mutant cells. Addition of... Tipo: Journal article Palavras-chave: Enthalpy; Entropy; Enzyme kinetics; Fermentation; Induction; Thermodynamics. Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200007 Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading Electron. J. Biotechnol. Illanes,Andrés; González,José Miguel; Gómez,José Manuel; Valencia,Pedro; Wilson,Lorena. Particle size and enzyme protein loading are design parameters of enzyme immobilization affecting biocatalyst performance that can be varied within broad margins. Their effect on mass transfer limitations at different bulk penicillin G concentrations has been studied with glyoxyl agarose immobilized penicillin G acylase biocatalysts of average particle size of 5·10-5m and 10·10-4m at protein loadings from 15 to 130 mg/g gel. Internal diffusional restrictions were evaluated for such biocatalysts: Thiele modulus varied from 1.17 for the small particles at the lower protein load to 5.84 for the large particles at the higher protein load. Effectiveness factors at different bulk substrate concentrations were determined for all biocatalysts, values ranging from... Tipo: Journal article Palavras-chave: Diffusional restrictions; Enzyme kinetics; Immobilized enzyme; Penicillin G acylase; Penicillin hydrolysis. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100002 THERMOSTABILITY OF ANTIOXIDANT AND DETERIORATIVE ENZYMES FROM SOURSOP AND CASHEW APPLE JUICES Rev. Bras. Frutic. RABELO,MARCELA CRISTINA; BRITO,EDY SOUSA DE; MOURA,CARLOS FARLEY HERBSTER; OLIVEIRA,LUCIANA DE SIQUEIRA; MIRANDA,MARIA RAQUEL ALCÂNTARA DE. ABSTRACT This work aimed to evaluate the thermostability of antioxidant (superoxide dismutase - SOD, catalase - CAT and ascorbate peroxidase - APX) and deteriorative (guaiacol peroxidase - G-POD, polyphenoloxidase - PPO, pectin-methylesterase - PME and polygalacturonase - PG) enzymes from soursop and cashew apple juices. Juices were prepared homogenizing ripe fruit pulps and submitting to different thermal treatments (55, 65, 75, 85 and 95°C) for different time period (1, 3, 5, 10, 15, 20 and 30 min) then enzymatic activities were evaluated. The treatments at 55°C for soursop juice and at 75°C for cashew apple juice presented the best results, considering the low residual activities for deteriorative enzymes and the retained activity of SOD. Our results... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pasteurization; Enzyme kinetics; Thermal inactivation; Annona muricata; Anacardium occidentale. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000200801 Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1 Rev. Microbiol. Maia,Maria de Mascena Diniz; Morais,Marcia Maria Camargo de; Morais Jr.,Marcos Antonio de; Melo,Eduardo Henrique Magalhães; Lima Filho,José Luiz de. A Brazilian strain of Fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.l-1 of lipase after 72 hours of cultivation at 25oC in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. Glucose (1% w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30oC and a good enzyme stability (80% activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50oC. Lipase... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme kinetics; Ester hydrolysis; Extracellular lipase; Fusarium solani. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400003 Assessment and kinetics of soil phosphatase in Brazilian Savanna systems Anais da ABC (AABC) FERREIRA,ADÃO S.; ESPÍNDOLA,SUÉLLEN P.; CAMPOS,MARIA RITA C.. The activity and kinetics of soil phosphatases are important indicators to evaluate soil quality in specific sites such as the Cerrado (Brazilian Savanna). This study aimed to determine the activity and kinetic parameters of soil phosphatase in Cerrado systems. Soil phosphatase activity was assessed in samples of native Cerrado (NC), no-tillage (NT), conventional tillage (CT) and pasture with Brachiaria brizantha (PBb) and evaluated with acetate buffer (AB), tris-HCl buffer (TB), modified universal buffer (MUB) and low MUB. The Michaelis-Menten equation and Eadie-Hofstee model were applied to obtain the kinetic parameters of soil phosphatase using different concentrations of p-nitrophenol phosphate (p-NPP). MUB showed the lowest soil phosphatase activity... Tipo: Info:eu-repo/semantics/article Palavras-chave: Soil phosphatase activity; Buffer; Enzyme kinetics; Michaelis-Menten equation; Eadie-Hofstee model; Cerrado. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000301035 Immobilization of lipase from Fusarium solani FS1 BJM Knight,Kirsty; Pimentel,Maria do Carmo Barros; Morais,Marcia Maria Camargo de; Ledingham,William M.; Lima Filho,José Luiz de; Maia,Maria de Mascena Diniz. Lipase from Fusarium solani FS1 was immobilized by covalent attachment to polyacrylamide beads and onto magnetized Dacron, retaining 12% and 97% of activity, respectively. Lipase was also entrapped within polyacrylamide beads, retaining 53% of activity. Investigations of the kinetic characteristics of the immobilized derivatives using triolein as substrate showed that lipase immobilized onto polyacrilamide beads and Dacron did not follow Michaelis-Menten kinetics. Tipo: Info:eu-repo/semantics/other Palavras-chave: Fusarium solani FS1; Immobilized lipase; Enzyme kinetics; Dacron; Polyacrylamide beads. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300013 Biological evaluation of 2,3-dioxoindolin-N-phenylacetamide derivatives as potent CDC25B and PTP1B phosphatase inhibitors BJPS Jin,Qing-Hao; Chen,Wen-Bo; Xia,Ya-Nan; Liu,Bing-Yu; Guan,Li-Ping. A series of 2,3-dioxoindolin-N-phenylacetamide derivatives was evaluated for inhibitory activity against CDC25B and PTP1B enzymes. Most of the derivatives showed inhibitory activity against CDC25B (IC50 = 3.2-23.2 µg/mL) and PTP1B (IC50 = 2.9-21.4 µg/mL). Compound 2h showed the most inhibitory activity in vitro with IC50 values of 3.2 and 2.9 µg/mL against CDC25B and PTP1B, respectively, compared with the reference drugs Na3VO4 (IC50 = 2.7 µg/mL) and oleanolic acid (IC50 = 2.3 µg/mL). The results of selectivity experiments showed that the 2,3-dioxoindolin-N-phenylacetamide derivatives were selective inhibitors against CDC25B and PTP1B. Enzyme kinetic experiments demonstrated that compound 2h was a specific inhibitor with the typical characteristics of a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Dioxoindolin-N-phenylacetamide; CDC25B; PTP1B; Phosphatase inhibitors; Enzyme kinetics. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100500 Registros recuperados: 7 Primeira ... 1 ... Última

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