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| Registros recuperados: 138 | |
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| SANTOS,Caroline Menicoze dos; RIBEIRO,Amanda da Silva; GARCIA,Adriana; POLLI,Andressa Domingos; POLONIO,Julio Cesar; AZEVEDO,João Lúcio; PAMPHILE,João Alencar. |
| ABSTRACT Endophyte microorganisms have great biotechnological interest, with features applicable to different areas and are potentially useful in agriculture. The current study determines the biotechnological potential of endophytic fungi, isolated from leaves of Sapindus saponaria, to control phytopathogenic fungi and evaluate their enzyme production. Molecular taxonomy was performed by sequencing of the ITS1-5.8S-ITS2 ribosomal DNA region, identifying the genera Phomopsis, Sordariomycetes, Diaporthe, and Colletotrichum. In vitro antagonism against phytopathogens showed better results against Fusarium solani and provided inhibition indices between 41.8 % and 67.5 %. The endophytic strain SS81 (Diaporthe citri) presented the highest antagonism index... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological control; Endophytes; Enzymes; Microbial antagonisms; Molecular taxonomy. |
| Ano: 2019 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000200322 |
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| Pádua,Ana Patrícia Sousa Lopes de; Freire,Karla Torres Lins de Sousa; Oliveira,Thays Gabrielle Lins de; Silva,Leticia Francisca da; Araújo-Magalhães,Gianne Rizzuto; Agamez-Montalvo,Gualberto Segundo; Silva,Iolanda Ramalho da; Bezerra,Jadson Diogo Pereira; Souza-Motta,Cristina Maria de. |
| ABSTRACT We studied the fungal endophyte community of the leaves of Myracrodruon urundeuva in Brazil along with their potential to produce L-asparaginase. In total, 187 endophytes were isolated. The colonization rate of leaf fragments from caatinga (10.41 %) was lower than those from brejo de altitude (39.58 %). Sequences of ITS rDNA from all endophytes revealed relationships with Ascomycota (Botryosphaeriales, Chaetothyriales, Diaporthales, Eurotiales, Glomerellales, Hypocreales, and Pleosporales) and Basidiomycota (Polyporales). The most frequent endophytes were members of the genus Diaporthe. Talaromyces was an indicator genus for caatinga while Phyllosticta, Diaporthe, and Colletotrichum were for brejo de altitude. The composition of endophytic fungi... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian ecosystems; Diaporthe; Endophytes; Enzymes; Fungal richness. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000100039 |
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| Nery, Marcela Carlota; Rocha, Adriana de Souza; Pinho, Édila Vilela de Resende Von; Santos, Heloisa Oliveira dos; Fialho, Cintia Maria Teixeira; Nery, Fernanda Carlota. |
| The seeds of a sesame crop contain approximately 50% oil content, one of the main reasons for their cultivation. However, information regarding methodologies for the assessment of quality seeds of this culture are scarce. Therefore, the objective of this study was to adapt the methods of accelerated ageing tests for sesame seeds and investigate changes in the behaviour of isoenzymes. The physiological characterization of the seeds and electrophoretic analysis of the isoenzymes superoxide dismutase, esterase, catalase, alcohol dehydrogenase, malate dehydrogenase and isocitrate lyase were also performed. For the accelerated ageing test, the seeds were subjected to the traditional method, with a saturated NaCl solution, at ageing times of 0, 24, 48, 72, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymes; Oilseed; Vigour; Quality; Sesamum indicum.; Produção e tecnologia de sementes. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39449 |
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| Silva, Leandro Moreira; Instituto Federal Minas Gerais - Campus Bambuí; Geraldo, Adriano; Instituto Federal Minas Gerais - Campus Bambuí; Vieira Filho, Javer Alves; Instituto Federal Minas Gerais - Campus Bambuí; Machado, Luiz Carlos; Instituto Federal Minas Gerais - Campus Bambuí; Brito, Jerônimo Ávito Gonçalves de; Universidade Federal do Recôncavo Baiano; Bertechini, Antonio Gilberto; Universidade Fderal de Lavras. |
| O objetivo do estudo foi avaliar o efeito da associação de carboidrases e fitase em dietas de poedeiras. Foram utilizadas 300 poedeiras Isa Brown com 26 semanas, submetidas aos tratamentos: Controle Positivo: sem enzimas e valorização dos nutrientes; T2: valorização em 1,5 e 6% a EMA (kcal kg-1), do milho e o farelo de soja, respectivamente, e redução nos níveis nutricionais conforme a matriz nutricional para a fitase; T3: valorização da EMA do milho e o farelo de soja (idem T2) e redução nos níveis de cálcio e fósforo conforme matriz nutricional da fitase; T4: T2 suplementado com 100 g t-1 de carboidrases e 30 g t-1 de fitase; T5: T3 suplementado com 100 g t-1 de carboidrases e 30 g t-1 de fitase. Foi utilizado delineamento inteiramente casualizado, seis... |
| Tipo: Pesquisa de campo |
Palavras-chave: 5.04.03.00-1 desempenho; Qualidade de ovos; Enzimas energia metabolizável; Fósforo disponível; Valorização de dietas performance; Egg quality; Enzymes; Metabolizable energy; Disponible phosphorus; Valorization of diets. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/12735 |
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| Vieira Filho, Javer Alves; Geraldo, Adriano; Machado, Luiz Carlos; Brito, Jerônimo Ávito Golçalvez de; Bertechini, Antônio Gilberto; Murakami, Elise Saori Floriano. |
| The experiment was conducted with the objective of evaluating the effect of protease enzyme supplementation on performance parameters and quality shell of laying hens. We used 240 Isa Brown commercial laying hens with 44 weeks of age. A completely randomized split-plot design (5 periods of 21 days) with four treatments and six replications (10 hens per replication) was used. The experimental diets were formulated according to the requirements of the breed. The following parameters were evaluated: egg production, feed intake, feed conversion, average egg weight, egg loss, specific gravity, percentage and shell thickness. After collection, the data were analyzed with SISVAR Statistical Package, and the means compared by SNK test at 5% probability. It was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laying hen farming; Enzymes; Grains; Nutrition. |
| Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/22830 |
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| Nolan, Pearl; Doyle, Evelyn; O'Kiley, Padraig. |
| The aims of this study were to quantify the effects of fibrolytic enzyme additives applied to each of four consecutive cuts of unwilted grass at ensiling on ensilage characteristics and specific CH4 yields (SMY) per unit mass and per unit land area. In light of the importance of the primary growth yield, the effects of the timing of Cut 1 were also investigated. Furthermore, the mass-SMY and area-SMY effect of any effluent produced during ensilage was determined.At each of four cuts that comprised annual growth, samples from four replicate plots of Lolium perenne and of Phleum pratense were untreated (control) or were treated with either of two fibrolytic enzymes (ENZ 1 and ENZ 2) prior to ensiling for 120 days. The mass-SMY of silages and effluents were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grass yield; Silage; Fibre; Enzymes; Effluent; CH4 yield per unit land area. |
| Ano: 2018 |
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4159 |
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| ARAÚJO,FÁBIO V. DE; NETTO,MARCELLE C.M.; AZEVEDO,GUSTAVO P.; JAYME,MARCELLY M.A.; NUNES-CARVALHO,MONICA C.; SILVA,MARIANA M.; CARMO,FLÁVIA L. DO. |
| ABSTRACT Marine sponges has been a large reservoir of microbial diversity, with the presence of many species specific populations as well as producing biologically active compounds, which has attracted great biotechnological interest. In order to verify the influence of the environment in the composition of the bacterial community present in marine sponges and biotechnological potential of bacteria isolated from these organisms, three species of sponges and the waters surrounding them were collected in different beaches of Rio de Janeiro, Brazil. The profile of the bacterial community present in sponges and water was obtained by PCR-DGGE technique and the biotechnological potential of the strains isolated by producing amylase, cellulase, protease and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diversity; Biosurfactants; Enzymes; Marine sponges; PCR-DGGE.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602785 |
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| TEIXEIRA,ANITA F.S.; SILVA,JACQUELINE S.; VILELA,LAÍZE A.F.; COSTA,PATRÍCIA F.; COSTA,ELAINE M. DA; GUIMARÃES,AMANDA A.; SANTOS,JESSÉ V. DOS; SILVA,SÉRGIO H.G.; CARNEIRO,MARCO AURÉLIO C.; MOREIRA,FATIMA M.S.. |
| Abstract: Several microbiological indicators of soil quality present high sensitivity, but little is known about the influence of topographic factors on them. This work aimed to evaluate variability of biological indicators of soil quality across a hillslope under native forest and the influence of topographic factors on them. Four positions on a hillslope were evaluated. Activity of the enzymes β-glucosidase, acid phosphatase, urease and fluorescein diacetate (FDA) hydrolysis were determined, as well as basal and substrate-induced respiration, and density of microorganisms: total bacteria, total fungi, actinobacteria, phosphate solubilizers, ammonifiers, native rhizobia, free-living N2-fixing bacteria, spores of arbuscular mycorrhizal fungi and percentage... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological indicators; Digital elevation model; Enzymes; Microbial activity; Random forest. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700866 |
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| CASELI,LUCIANO. |
| ABSTRACT In this review we discuss about the immobilization of enzymes in Langmuir-Blodgett films in order to determine the catalytic properties of these biomacromolecules when adsorbed on solid supports. Usually, the conformation of enzymes depends on the environmental conditions imposed to them, including the chemical composition of the matrix, and the morphology and thickness of the film. In this review, we show an outline of manuscripts that report the immobilization of enzymes as LB films since the 1980’s, and also some examples of how the surface properties of the floating monolayer prepared previously to the transfer to the solid support are important to determine the efficiency of the resulting device. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Langmuir-Blodgett; Enzymes; Biosensors; Air-water interface. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000200631 |
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| SOBRAL,LAUREANA V.; MELO,KELLY N.; SOUZA,CLECIANA M.; SILVA,SÍLVIO F.; SILVA,GILVANIA L.R.; SILVA,ANDRESSA L.F.; WANDERLEY,KATHARINE A.A.; OLIVEIRA,IDJANE S.; CRUZ,ROBERTA. |
| ABSTRACT To the fungal microbiota the UFPE and biotechnological potential enzymatic and antimicrobial production. Air conditioned environments were sampled using a passive sedimentation technique, the air I ratio and the presence of aflatoxigenic strains evaluated for ANVISA. Icelles were to determine the enzymatic activity of lipase, amylase and protease metabolic liquids to determine antimicrobial activity. Diversity was observed in all CAV environments, CFU/m3 ranged from 14 to 290 and I/E ratio from 0.1 to 1.5. The of the fungal genera were: Aspergillus (50%), Penicillium (21%), Talaromyces (14%), Curvularia and Paecilomyces (7% each). Aspergillus sydowii (Bainier & Sartory) Thom & Church presented enzymatic activity and the Talaromyces... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Air quality; Bacteria; Enzymes; Filamentous fungi; Refrigerated environments. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502327 |
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| ALVES,CARLA; COSTA,EDUARDA; SOFIATTI,JESSICA R.; FORTE,CESAR T.; WINTER,FÁBIO L.; HOLZ,CINTHIA M.; KAIZER,ROSILENE R.; GALON,LEANDRO. |
| ABSTRACT Most herbicides applied in crop field, stay in the soil for a period, affecting next crop or even the plants using as green manure. Nowadays, the use of herbicides grow to increase productivity, mainly in the grain producing region north of Rio Grande do Sul state. The objective of this study was to evaluate the effects of herbicides fomesafen and sulfentrazone on antioxidant system in Avena sativa1, Vicia sativa2, Raphanus sativus and Lupinus albus. The plants were exposed to varying concentrations of fomesafen3 (0, 0.125, 0.25 and 0.5 kg ha -1) and sulfentrazone (0, 0.3, 0.6 and 1.2 kg ha-1). For this, the activities of, ascorbat peroxidase, catalase and guaiacol enzymes were analyzed, and the levels of MDA were quantificated. Fomesafen and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catalase; Enzymes; Agricultural plants; PROTOX system. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401533 |
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| Teixeira-Neto,A.R.; Ferraz,G. C.; Moscardini,A. R. C.; Balsamão,G. M.; Souza,J. C. F.; Queiroz-Neto,A.. |
| This study tried to monitor the alterations of muscle enzymes activity - creatinokinase (CK), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST) - in a group of horses that participated of 70 and 100km distance rides in five competitions of an annual endurance championship under tropical climate. Pre ride levels (U/l) were 245.13±9.84 for CK, 496.61±14.76 for LDH, and 328.95±8.65 for AST. When compared to these levels, the results revealed a significant decrease in all enzymes activities in the first moment of the rides. Peak levels, significantly different, were reached, immediately after rides by CK (413.591±50.75); 24 hours post rides by LDH (628.61±33.30); and 48 hours after rides by AST (389.89±16.96). Monitoring of recovery period... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Horse; Endurance; Enzymes; Muscle. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300004 |
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| Oliveira,M.C.; Rodrigues,E.A.; Marques,R.H.; Gravena,R.A.; Guandolini,G.C.; Moraes,V.M.B.. |
| The performance and the morphology of intestinal mucosa of broilers fed mannan-oligosaccharides (MOS) and enzymes (E) from one to 21-day-old were evaluated using 750 one-day-old chicks, assigned to a 2 x 2 + 1 factorial design - two levels of MOS (0 and 0.1%), two levels of E (0 and 0.05%) plus an antibiotic positive control diet - performing five treatments of five replications each one. MOS x E interaction was significant for both duodenal (P<0.002 and P<0.002) and ileal (P<0.04 and P<0.05) perimeters and heights of villi, being the values lower in the mucosa of birds fed non-supplemented diets. MOS based-diet determined an increase on perimeter of jejunal villi (P<0.05). Compared with antibiotic treatment group, villi perimeter... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler; Nutrition; Enzymes; Prebiotic. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200025 |
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| Melo,J.F.B.; Lundstedt,L. M; Inoue,L.A.K.; Metón,I.; Baanante,I.V.; Moraes,G.. |
| ABSTRACT The activities of enzymes from a number of metabolic pathways have been used as a tool to evaluate the best use of nutrients on fish performance. In the present study the catfish Rhamdia quelen was fed with diets containing crude protein-lipid-carbohydrate (%) as follows: treatment (T) T1: 19-19-44; T2: 26-15-39; T3: 33-12-33; and T4: 40-10-24. The fish were held in tanks of re-circulated, filtered water with controlled temperature and aeration in 2000L experimental units. The feeding experiment lasted 30 days. The following enzymes of the carbohydrate metabolism were determined: Glucokinase (GK), Phosphofructokinase 1 (PFK-1), Pyruvate kinase (PK), Fructose-1,6-biphosphatase 1 (FBP-1). The activities of 6 phosphogluconate dehydrogenase (6PGDH)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metabolism; Enzymes; Nutrition; Adaptation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501251 |
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| Coutinho,S.D.A.. |
| The relationship between production of enzymes and pathogenicity of Malassezia pachydermatis strains was studied. Thirty strains of M. pachydermatis isolated from dogs were used: 15 originating from animals with external otitis and 15 obtained from ears of asymptomatic dogs. The samples, obtained by introduction of a sterile swab into the auditory canal after cleaning the auricle with alcohol-ether solution, were seeded onto Sabouraud dextrose agar with chloramphenicol (100mg/l) and were incubated at 37°C for up to two weeks. The colonies were identified according to their macro-and-micromorphology. All strains were investigated for the production of chondroitin-sulphatase, hyaluronidase, phospholipase and proteinase. Most of the strains showed a high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Malassezia pachydermatis; Enzymes; Otitis; Microbiota. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800003 |
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| Tripathi,G; Kachhwaha,N.; Dabi,I.; Bandooni,N.. |
| The effects of varying temperatures (12 - 44° C) on the specific activity of cytoplasmic malate dehydrogenase ((cMDH), mitochondrial malate dehydrogenase (mMDH) and lactate dehydrogenase (LDH) of some earthworms (Metaphire posthuma, Perionyx sansibaricus and Lampito mauritii) were studied. The effects of different temperatures on supernatant and mitochondrial protein contents were also investigated. The specific activities of cMDH, mMDH and LDH of the earthworms decreased gradually as a function of increasing temperature from 12 to 44°C. Higher metabolic energy was needed to maintain the activity at low temperatures. Hence, the earthworms showed increased enzyme specific activity at low temperatures. However, the protein content increased upto 28°C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymes; Proteins; Earthworms; Temperatures. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400017 |
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| Registros recuperados: 138 | |
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