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Preparation techniques alter the mineral and organic fractions of fish otoliths: insights using Raman micro-spectrometry ArchiMer
Jolivet, Aurelie; Fablet, Ronan; Bardeau, Jean-francois; De Pontual, Helene.
The high spatial resolution analysis of the mineral and organic composition of otoliths using Raman micro-spectrometry involves rigorous protocols for sample preparation previously established for microchemistry and trace elements analyses. These protocols often include otolith embedding in chemically neutral resin (i.e., resins which do not contain, in detectable concentration, elements usually sought in the otoliths). Such embedding may however induce organic contamination. In this paper, Raman micro-spectrometry reveals the presence of organic contamination onto the surface obtained from the use of epoxy resin, specifically Araldite. This contamination level varies depending on otolith structures. Core and checks, known as structural discontinuities,...
Tipo: Text Palavras-chave: Epoxy resin; Otolith composition; Microstructure; Raman spectrometry.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00138/24965/26372.pdf
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Report on a Sheet Plastination Technique using Commercial Epoxy Resin International Journal of Morphology
Ottone,Nicolás Ernesto; del Sol,Mariano; Fuentes,Ramón.
Plastination is a conservation technique which allows anatomical pieces to be preserved, dry and odor-free, for an indefinite period. In particular, plastination of sections of tissue with epoxy resin allows very thin slices to be made of various regions of the anatomy, permitting close viewing of anatomical structures which are difficult to access by dissection or cadaver exploration. The objective of this work is to present a plastination technique developed in our laboratory for tissue sections using commercial epoxy resin, as an alternative to the existing classic plastination techniques. The technique was applied to a human knee, obtaining 5 mm thick sections which were compared with computerized tomography images. The development of an alternative...
Tipo: Journal article Palavras-chave: Sheet plastination; Epoxy resin.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300036
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