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Enhanced Production Of Streptokinase By Chemical Mutagenesis Of Streptococcus agalactiae EBL-20 BABT
Arshad,Arooj; Zia,Muhammad Anjum; Asghar,Muhammad; Joyia,Faiz Ahmad.
Abstract Streptokinase (SK) is an enzyme that is used for the treatment of cardiovascular diseases. The current study focused on the enhanced production of SK by inducing mutation in Streptococcus agalactiae EBL-20 and optimization of medium components and culture conditions for the maximum growth of mutant derived strain. S. agalactiae EBL-32 was selected as a potent mutant after exposure of S. agalactiae EBL-20 to EMS for 180 minutes. SK activity obtained from mutant derived strain was found to be 1.6 fold higher as compared to the activity achieved by wild strain. Nutritional requirements of the mutated strain were optimized by single factor analysis method suggesting glucose as the optimum carbon source; yeast extract and peptone as a suitable nitrogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethyl methanesulfonate; Fermentation; Response surface methodology; Streptokinase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100200
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Increased expression of keratinase and other peptidases by Candida parapsilosis mutants BJMBR
Duarte,T.R.; Oliveira,S.S.; Macrae,A.; Cedrola,S.M.L.; Mazotto,A.M.; Souza,E.P.; Melo,A.C.N.; Vermelho,A.B..
Keratinases are enzymes of great importance involved in pathogenic processes of some fungi. They also have a widespread ecological role since they are responsible for the degradation and recycling of keratin. On the one hand, studying them furthers our knowledge of pathogenicity mechanisms, which has important implications for human health, and on the other hand, understanding their ecological role in keratin recycling has biotechnological potential. Here, a wild-type keratinolytic Candida parapsilosis strain isolated from a poultry farm was treated with ethyl methanesulfonate in order to generate mutants with increased keratinase activity. Mutants were then cultured on media with keratin extracted from chicken feathers as the sole source of nitrogen and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida parapsilosis; Keratinase; Ethyl methanesulfonate; Mutants.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300006
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Lipopeptide production by Bacillus subtilis R1 and its possible applications BJM
Jha,Sujata S.; Joshi,Sanket J.; S.J.,Geetha.
Abstract The possible application of a bacterial strain - Bacillus subtilis R1, isolated from an oil contaminated desert site in India, as biocontrol agent and its biosurfactant in microbial enhanced oil recovery are discussed. The biosurfactant production in minimal medium was carried out at different temperatures and salt concentrations, where it produced an efficient biosurfactant at 30-45 °C and in presence of up to 7% salt. It significantly reduced the surface tension from 66 ± 1.25 mN/m to 29 ± 0.85 mN/m within 24 h. In order to enhance the biosurfactant production, random mutagenesis of B. subtilis R1 was performed using chemical mutagen - ethyl methanesulfonate. Majority of the isolated 42 mutants showed biosurfactant production, but the difference...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus subtilis; Biosurfactant; Ethyl methanesulfonate; Microbial enhanced oil recovery; Antifungal activity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400955
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