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Simultaneous amyloglucosidase and exo-polygalacturonase production by Aspergillus niger using solid-state fermentation BABT
Costa,Jorge Alberto Vieira; Colla,Eliane; Magagnin,Glênio; Santos,Lucielen Oliveria dos; Vendruscolo,Mauricio; Bertolin,Telma Elita.
Amyloglucosidase (AMG) and exo-polygalacturonase (exo-PG) were simultaneously produced by two different strains of Aspergillus niger in solid-state fermentation (SSF) using defatted rice-bran as substrate. The effect of Aspergillus niger strain (t0005/007-2 and/or CCT 3312), inoculum type (spore suspension or fermented bran) and addition of inducers (pectin and/or starch) to the culture media was studied using a 3² x 2¹ factorial experimental design. The production of AMG and exo-PG was significantly affected by fungal strain and inoculum type but inducers had no effect. The maximum yields obtained were 1310 U/g dm for AMG using a spore suspension of A. niger CCT 3312 and 50.2 U/g dm for exo-PG production, using A. niger t0005/007-2 and fermented bran as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amyloglucosidase; Exo-polygalacturonase; Rice-bran; Solid-state fermentation.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500003
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Exo-polygalacturonase production by Bacillus subtilis CM5 in solid state fermentation using cassava bagasse BJM
Swain,Manas R.; Kar,Shaktimay; Ray,Ramesh C..
The purpose of this investigation was to study the effect of Bacillus subtilis CM5 in solid state fermentation using cassava bagasse for production of Exo-polygalacturonase (exo-PG). Response surface methodology was used to evaluate the effect of four main variables, i.e. incubation period, initial medium pH, moisture holding capacity (MHC) and incubation temperature on enzyme production. A full factorial Central Composite Design was applied to study these main factors that affected exo-PG production. The experimental results showed that the optimum incubation period, pH, MHC and temperature were 6 days, 7.0, 70% and 50ºC, respectively for optimum exo-PG production.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Exo-polygalacturonase; Bacillus subtilis CM5; Response surface methodology; Solid state fermentation; Cassava bagasse.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300028
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