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	Provedor de dados ArchiMer (2) Electron. J. Biotechnol. (1) Autor Bowman, John (1) Choorit,Wanna (1) Combes, D (1) Compere, Chantal (1) Delbarre Ladrat, Christine (1) Ghillebaert, F (1) Guezennec, Jean (1) Leroy, Celine (1) Lungmann,Patcharee (1) Nichols, Carol (1) Prasertsan,Poonsuk (1) Mais... Palavra-chave Exopolymer (3) Antarctica (1) Antifouling (1) Biofilm (1) Capsular protein (1) EPS yield (1) Exopolysaccharides (1) Halophilic bacteria (1) Marine bacteria (1) Medium optimization (1) Mixture design (1) Pseudoalteromonas (1) Psychrotolerant (1) Response surface method (1) Sea ice (1) Subtilisin (1) Mais... Tipo do documento Text (2) Journal article (1) Ano 2008 (1) 2007 (1) 2005 (1) País France (2) Chile (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Application of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. SM5 Electron. J. Biotechnol. Lungmann,Patcharee; Choorit,Wanna; Prasertsan,Poonsuk. The study investigated on medium optimization for production of exopolymer by a newly isolated bacterium, Halobacterium sp. SM5, using the mixture design and response surface method. The mixture experiment was designed by setting five parameters at the lowest and highest of concentrations: pseudo components design containing 0.15-0.25 glucose, 0.15-0.25 yeast extract, 0.35-0.45 MgSO4·7H2O, 0.1-0.2 vitamin casamino acid and 0.02-0.06 KCl. The results of the mixture design revealed that the effect of nutrients or elements on the exopolymer produced by the strain SM5 were in the order of MgSO4·7H2O > yeast extract > vitamin casamino acid > KCl > glucose, respectively. The suitable medium recipe for enhancement the exopolymer production was 7.43... Tipo: Journal article Palavras-chave: Exopolymer; Halophilic bacteria; Medium optimization; Mixture design; Response surface method. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100001 Influence of subtilisin on the adhesion of a marine bacterium which produces mainly proteins as extracellular polymers ArchiMer Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Compere, Chantal; Combes, D. Aims: The nature of exopolymers involved in the adhesion of a marine biofilm-forming bacterium Pseudoalteromonas sp. D41 was investigated to evaluate and understand the antifouling potential of subtilisin. Methods and Results: The exopolymers of D41 produced by fermentation were analysed by FTIR and SDS-PAGE showing the presence of polysaccharides, glycoproteins and proteins. A high content of proteins was detected both in soluble and capsular fractions. The microscopic observations of fluorescamine and calcofluor stained adhered D41 indicated mainly the presence of proteins in exopolymers produced during adhesion. Subtilisin, the broad spectrum protease, tested in natural sea water and in polystyrene microplates showed that antifouling activity was... Tipo: Text Palavras-chave: Subtilisin; Pseudoalteromonas; Exopolymer; Capsular protein; Biofilm; Antifouling. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4624.pdf Effects of incubation temperature on growth and production of exopolysaccharides by an Antarctic sea ice bacterium grown in batch culture ArchiMer Nichols, Carol; Bowman, John; Guezennec, Jean. The sea ice microbial community plays a key role in the productivity of the Southern Ocean. Exopolysaccharide (EPS) is a major component of the exopolymer secreted by many marine bacteria to enhance survival and is abundant in sea ice brine channels, but little is known about its function there. This study investigated the effects of temperature on EPS production in batch culture by CAM025, a marine bacterium isolated from sea ice sampled from the Southern Ocean. Previous studies have shown that CAM025 is a member of the genus Pseudoalteromonas and therefore belongs to a group found to be abundant in sea ice by culture-dependent and -independent techniques. Batch cultures were grown at -2 degrees C, 10 degrees C, and 20 degrees C, and cell number, optical... Tipo: Text Palavras-chave: EPS yield; Psychrotolerant; Sea ice; Marine bacteria; Antarctica; Exopolymer; Exopolysaccharides. Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-625.pdf Registros recuperados: 3 Primeira ... 1 ... Última

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