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	Provedor de dados BJM (4) Ciênc. Tecnol. Aliment. (3) Biological Sciences (3) Anais da ABC (AABC) (2) BJPS (1) Scientia Agricola (1) Ciência Rural (1) Electron. J. Biotechnol. (1) AGRIAMBI (1) Mais... Autor Baldo, Cristiani (2) Celligoi, Maria Antonia Pedrine Colabone (2) ALVARENGA,Flávia Barbosa Magalhães (1) ARCANJO,Narciza Maria de Oliveira (1) AZEVEDO,MARIANE M. (1) Akingbala,Oluwatomi (1) Amaral,André Cestonaro do (1) BEZERRA,Taliana Kênia Alvez (1) Batistela,Vagner R. (1) Benazzi,Toni Luis (1) Bezerra,Raquel P (1) Borsari,Raquel Renan Jorge (1) Buzato, João Batista (1) Buzato,João Batista (1) CAMPOS,Náira da Silva (1) Caetano,Wilker (1) Calazans,G.M.T. (1) Carneiro,Eliana Beleski Borba (1) Celligoi,Maria Antonia P. Colabone (1) Cruz Filho,Raimundo F. (1) Mais... Palavra-chave Factorial design (17) Sucrose (2) 90400003 enzyme (1) Acetic fermentation (1) Acid hydrolysis (1) Actinomycetes (1) Agro-industrial waste (1) Amazonian soil (1) Antioxidant capacity (1) Apparent sorption coefficient (1) Artemia salina (1) Asparagine (1) Aspergillus niger (1) Bacillus sp (1) Batch (1) Batch experiment (1) Batch system (1) Bioassay (1) Bioquímica de microrganismos physical factors (1) Carotenoids (1) Mais... Tipo do documento Info:eu-repo/semantics/article (16) Journal article (1) Ano 2018 (2) 2017 (1) 2016 (3) 2015 (4) 2013 (2) 2012 (2) 2007 (1) 2006 (1) 2005 (1) Mais... País Brazil (16) Chile (1) Idioma Inglês (17)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 17 Primeira ... 1 ... Última Effect of fermentation conditions on the production of hyaluronic acid by Streptococcus zooepidemicus ATCC 39920 Biological Sciences Pan, Nicole Caldas; Vignoli, Josiane Alessandra; Baldo, Cristiani; Pereira, Hanny Cristina Braga; Silva, Rui Sérgio dos Santos Ferreira; Celligoi, Maria Antonia Pedrine Colabone. The production of hyaluronic acid by Streptococcus zooepidemicus ATCC 39920 with varying rates of pH (6.0, 7.0, 8.0), temperature (34; 37; 40°C), agitation (100, 150, 200 rpm), glucose (10, 20, 30 g L-1) and yeast extract concentration (10, 20, 30 g L-1) was evaluated by statistical approaches. The best conditions for the production of hyaluronic acid was pH 8.0, 37°C and 100 rpm in a medium containing 30 g L-1 glucose and yeast extract, for a production of 0.787 g L-1. Temperature, pH and yeast extract were significant variables (p < 0.05). Yeast extract and pH had a positive effect on the production of the polymer. Lactate, formate and acetate synthesis were also analyzed. Current assay showed the feasibility of statistical tools to optimize the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioquímica de microrganismos physical factors; Nutritional factors; Glycosaminoglycans; Factorial design; Microbial production. Fermentação. Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28176 Optimization of asparaginase production from Zymomonas mobilis by continuous fermentation Biological Sciences Menegat, Francieli Bortoluzzi; Baldo, Cristiani; Celligoi, Maria Antonia Pedrine Colabone; Buzato, João Batista. Asparaginase is an enzyme used in clinical treatments as a chemotherapeutic agent and in food technology to prevent acrylamide formation in fried and baked foods. Asparaginase is industrially produced by microorganisms, mainly gram-negative bacteria. Zymomonas mobilis is a Gram-negative bacterium that utilizes glucose, fructose and sucrose as carbon source and has been known for its efficiency in producing ethanol, sorbitol, levan, gluconic acid and has recently aroused interest for asparaginase production. Current assay optimizes the production of Z. mobilis asparaginase by continuous fermentation using response surface experimental design and methodology. The studied variables comprised sucrose, yeast extract and asparagine. Optimized condition obtained... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sucrose; Asparagine; Yeast extract; Enzyme; Microbial production; Factorial design. Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31057 Reuse of wastewater from pulp industry for the optimization of fungal xylanase production Biological Sciences Queiroz-Fernandes, Geisiany Maria de; Martins, Bruna Letícia; Rustiguel, Cynthia Barbosa. The production of enzymes using agro-industrial waste is a low cost alternative for the reuse of byproducts, with the subsequent impact decrease on the environment. Current analysis produced xylanase using fungus Aspergillus niger, with two types of wastewater generated during the pulp chemical bleaching phase as inducers. Xylanase was produced by submerged liquid fermentation and factorial design optimized parameters that influence production (concentration of wastewater and production period). Initial culture conditions (pH, temperature and agitation) were optimized independently. Alkaline wastewater was more effective than acidic wastewater for the induction of xylanase in optimized conditions: 50% of culture medium, 7-day production, 30°C, pH 6.0 and... Tipo: Info:eu-repo/semantics/article Palavras-chave: 90400003 enzyme; Aspergillus niger; Factorial design; Agro-industrial waste; Xylanase. Biotecnologia. Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34459 Impact of percentage and particle size of sugarcane biochar on the sorption behavior of clomazone in Red Latosol Anais da ABC (AABC) SILVA,MARCOS R.F. DA; QUEIROZ,MARIA ELIANA L.R. DE; NEVES,ANTÔNIO A.; SILVA,ANTÔNIO A. DA; OLIVEIRA,ANDRÉ F. DE; OLIVEIRA,RENAN L. DE; AZEVEDO,MARIANE M.. Abstract Biochar is a carbonaceous material that has excellent potential as a fertilizer and soil conditioner. However, there is a lack of information concerning the effects of the amount and particle size of this pyrogenic material on the soil sorption capacity. In this work, evaluation was made of changes in clomazone (CMZ) sorption in a Red Latosol following soil conditioning using different percentages (0.25, 0.5, and 1% w/w) of sugarcane biochar in three particle sizes (<106, 106-508, and 508-610 µm). The conditioned soils presented apparent sorption coefficients (Kd) up to 1300 times higher than that of pure soil, besides changes in the behavior of CMZ sorption. The biochar particle size and percentage influenced sorption of the herbicide as well... Tipo: Info:eu-repo/semantics/article Palavras-chave: Apparent sorption coefficient; Batch experiment; External surface area; Factorial design; Freundlich isotherm; Herbicide retention. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703745 Evaluation of the photodynamic activity of Xanthene Dyes on Artemia salina described by chemometric approaches Anais da ABC (AABC) Pellosi,Diogo S.; Batistela,Vagner R.; Souza,Vagner R. de; Scarminio,Ieda S.; Caetano,Wilker; Hioka,Noboru. The development of drugs for photodynamic therapy (PDT) is an important area of research due to their growing use in medical applications. Therefore, it is important to develop new bioassay methods for PDT photosensitizers that are inexpensive, easy to handle and highly sensitive to environmental conditions. Xanthene dyes (fluorescein, rose bengal B, erythrosine B and eosin Y) with LED light sources were investigated using Artemia salina as a bioindicator of photodynamic activity. In this study, three factors were investigated: (i) photosensitizers concentration, (ii) the LED irradiation time and (iii) the waiting time between the addition of the photosensitizers and the beginning of the irradiation. To analyze the photo-killing of A. salina, it was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Artemia salina; Factorial design; Photodynamic therapy; Bioassay; Xanthenes. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401267 Production of mycelial biomass by the Amazonian edible mushroom Pleurotus albidus BJM Kirsch,Larissa de Souza; Macedo,Ana Júlia Porto de; Teixeira,Maria Francisca Simas. ABSTRACT Edible mushroom species are considered as an adequate source of food in a healthy diet due to high content of protein, fiber, vitamins, and a variety of minerals. The representatives of Pleurotus genus are characterized by distinct gastronomic, nutritional, and medicinal properties among the edible mushrooms commercialized worldwide. In the present study, the growth of mycelial biomass of Pleurotus albidus cultivated in submerged fermentation was evaluated. Saccharose, fructose, and maltose were the three main carbon sources for mycelial biomass formation with corresponding yields of 7.28 g L−1, 7.07 g L−1, and 6.99 g L−1. Inorganic nitrogen sources did not stimulate growth and the optimal yield was significantly higher with yeast extract (7.98 g... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pleurotus albidus; Submerged fermentation; Mycelial biomass; Factorial design. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300658 Protease with collagenolytic activity produced by Bacillus sp. DPUA 1728 from Amazonian soil BJM Lima,Lorena A.; Cruz Filho,Raimundo F.; Santos,Januário G. dos; Silva,Wilson C.. Qualitative analyses were carried out on solid medium with insoluble collagen 0.25% (w/v) to detect proteases with collagenolytic activity produced by Bacillus sp. In cultures incubated for 24 h, a 23 full factorial design with four repetitions at the center point was developed to analyze the effects and interactions between initial pH, temperature and the concentration of gelatin. Based on the results of the first 23 full factorial design, a successive 23 full factorial design was performed. The most favorable production conditions were found to be 1.5% (w/v) gelatin, pH 9.0 and 37 °C with enzymatic activity of 86.27 U/mL. The enzyme showed optimal activity at 50 °C and pH 9.0, and it was stable over wide pH (7.2-10.0) and temperature (45 °C-60 °C)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Collagenolytic protease; Bacillus sp; Factorial design; Amazonian soil. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401217 Evaluation of biomass production, carotenoid level and antioxidant capacity produced by Thermus filiformis using fractional factorial design BJM Mandelli,Fernanda; Yamashita,Fábio; Pereira,José L.; Mercadante,Adriana Z.. A fractional factorial design 2(5-1) was used to evaluate the effect of temperature, pH, and concentrations of yeast extract, tryptone and Nitsch's trace elements on the biomass, total carotenoids and protection against singlet oxygen by carotenoid extracts of the bacterium Thermus filiformis. In addition, the carotenoid composition was determined by high-performance liquid chromatography connected to a diode array and mass spectrometer detectors (HPLC-DAD-MS/MS). The production of biomass ranged from 0.113 to 0.658 g/L, the total carotenoid from 137.6 to 1,517.4 mg/g and the protection against singlet oxygen from 4.3 to 85.1 %. Results of the fractional factorial design showed that temperature had a negative effect on biomass production and a positive... Tipo: Info:eu-repo/semantics/article Palavras-chave: Thermophile bacterium; Carotenoids; HPLC-DAD-MS/MS; Antioxidant capacity; Factorial design. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100014 Application of fractional factorial design to levan production by Zymomonas mobilis BJM Melo,I.R.; Pimentel,M.F.; Lopes,C.E.; Calazans,G.M.T.. Levan is a non-toxic, biologically active, extra cellular polysaccharide composed solely by fructose units. Optimization of levan production by Zymomonas mobilis strain ZAG-12 employing a 2(4-1) fractional factorial design was performed to analyze the influence of the temperature (20, 25 e 30ºC) agitation (50, 75 e 100 rpm), and the initial concentrations of both sucrose (150, 200 e 250 g.L-1) and yeast extract (2.0, 3.5 e 5.0g.L-1) on final levan concentration. Aerobic fermentation was performed batchwise in 500mL Pyrex flasks for 72 hours. Biomass, ethanol, levan and sucrose were determined at beginning and also at end of the fermentations. The experiments showed that the final levan concentration depended on initial sucrose concentration, temperature... Tipo: Info:eu-repo/semantics/article Palavras-chave: Factorial design; Levan; Zymomonas mobilis. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100010 Characterization and evaluation of acid-modified starch of Dioscorea oppositifolia (Chinese yam) as a binder in chloroquine phosphate tablets BJPS Okunlola,Adenike; Akingbala,Oluwatomi. Chinese yam (Dioscorea oppositifolia) starch modified by acid hydrolysis was characterized and compared with native starch as a binder in chloroquine phosphate tablet formulations. The physicochemical and compressional properties (using density measurements and the Heckel and Kawakita equations) of modified Chinese yam starch were determined, and its quantitative effects as a binder on the mechanical and release properties of chloroquine phosphate were analyzed using a 2³ full factorial design. The nature (X1), concentration of starch (X2) and packing fraction (X3) were taken as independent variables and the crushing strength-friability ratio (CSFR), disintegration time (DT) and dissolution time (t80) as dependent variables. Acid-modified Chinese yam... Tipo: Info:eu-repo/semantics/article Palavras-chave: Acid hydrolysis; Chinese yam/starch; Chinese yam/binding properties; Factorial design; Drugs/release; Dioscorea oppositifolia/pharmaceutics. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400009 Optimization of the HS-SPME-GC/MS technique for determining volatile compounds in red wines made from Isabel grapes (Vitis labrusca) Ciênc. Tecnol. Aliment. ARCANJO,Narciza Maria de Oliveira; BEZERRA,Taliana Kênia Alvez; SILVA,Flávio Luís Honorato da; MADRUGA,Marta Suely. Abstract Brazilian wine production is characterized by Vitis labrusca grape varieties, especially the economically important Isabel cultivar, with over 80% of its production destined for table wine production. The objective of this study was to optimize and validate the conditions for extracting volatile compounds from wine with the solid-phase microextraction technique, using the response surface method. Based on the response surface analysis, it can be concluded that the central point values maximize the process of extracting volatile compounds from wine, i.e., an equilibrium time of 15 minutes, an extraction time of 35 minutes, and an extraction temperature of 30 °C. Esters were the most numerous compounds found under these extraction conditions,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Method; Factorial design; Grape; Validation. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000400676 Influence of carbon source and the fermentation process on levan production by Zymomonas mobilis analyzed by the surface response method Ciênc. Tecnol. Aliment. Borsari,Raquel Renan Jorge; Celligoi,Maria Antonia P. Colabone; Buzato,João Batista; Silva,Rui Sérgio dos Santos Ferreira da. The aim of this study is to assess sugar cane juice and sucrose as substrates, the batch and fed batch processes and their interaction in the levan production using a complete factorial design. Zymomonas mobilis was cultivated in different sugar cane juice and sucrose concentrations in two fermentation processes at 25 °C for 20 h. A complete factorial design (2³) was used to analyze the effects of the type and concentration of the substrate, as well as the batch and fed batch processes. A complete second factorial design (2²) was used to observe the importance of sugar cane juice. The results indicated that the batch process improved the levan production reaching 40.14 g/L. The addition of sugar cane juice was not statistically significant for levan... Tipo: Info:eu-repo/semantics/article Palavras-chave: Batch; Factorial design; Fed batch; Sucrose; Sugar cane juice. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612006000300019 Multivariate approach to assess in vitro Fe bioaccessibility in chicken meat Ciênc. Tecnol. Aliment. CAMPOS,Náira da Silva; LOURDES,Ângela Maria Ferreira de Oliveira; ALVARENGA,Flávia Barbosa Magalhães; SABARENSE,Céphora Maria; OLIVEIRA,Marcone Augusto Leal de; SOUSA,Rafael Arromba de. Abstract A 32 factorial design was employed to develop an in vitro digestion method for estimation of Fe bioaccessible fractions in cooked chicken meat. The effects of sample size and the in vitro bioaccessible fractions of this essential element were evaluated. A sample preparation method employing a microwave assisted digestion with dilute nitric acid was used prior to total Fe determination. For the bioacessibility studies, the optimized procedure employed 7.5 g of sample and 6% w/v of an acid pepsin solution. This procedure was applied to two kinds of chicken meat samples: breast and liver. Flame Atomic Absorption Spectrometry was used to determine total and bioaccessible (chyme or soluble portion) levels of iron in the samples. With respect to total... Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron bioaccessibility; Factorial design; Chicken meat; Microwave assisted digestion; Flame Atomic Absorption Spectrometry. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000100157 Hydrolyzed collagen, modified starch and guar gum addition in turkey ham Ciência Rural Prestes,Rosa Cristina; Carneiro,Eliana Beleski Borba; Demiate,Ivo Mottin. The main problems observed in cooked hams are bad sliceability and excessive fluid loss after cooking. To reduce these problems the industry uses non-meat ingredients such as soy protein and carrageenan, but under Brazilian law, it is not allowed to add starch or modified starch in hams. Three ingredients were tested in the present research: modified starch (0 to 2.0%), gum guar (0 to 0.30%) and hydrolyzed collagen (0 to 2.0%), following a 2³ full factorial design with five repetitions in the central point. The guar gum produced low resistance to reheating, however in losses by cooling, the results were adequate. The hydrolyzed collagen tested did not give satisfactory results, showing low acceptance due to formation of gel in the ham and high losses. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Texture; Factorial design; Non-meat ingredients. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000700027 Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens Electron. J. Biotechnol. M.M. Silva,Germana; Bezerra,Raquel P; Teixeira,José A; Porto,Tatiana S; Lima-Filho,José L; Porto,Ana Lúcia F. Background Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 2² factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentration. A polynomial model was fitted to correlate the relationship between the two variables and protease activity. In relation to fibrinolytic activity, the highest activity of 706.5 mm² was obtained at 1.7% soybean flour and 1.0% glucose concentration, which was 33% higher than plasmin. Fibrinolytic production was not optimized in the studied... Tipo: Journal article Palavras-chave: Actinomycetes; Factorial design; Glucose; Microbial enzymes; Soybean flour. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100004 Effect of nutrient sources on bench scale vinegar production using response surface methodology AGRIAMBI Ferreira,Joelma M.; Swarnakar,Ramdayal; Silva,Flávio L. H. da. The present work aims to evaluate on a bench scale, the effects of nitrogen and phosphorous nutrient source concentrations in vinegar production, a process that is used by small scale industries in the State of Paraiba. The response surface methodology has been utilized for modeling and optimization of the fermentation process. Initially a 2³ complete factorial design was used, where the effects of initial concentrations of ethyl alcohol, phosphorous and nitrogen sources were observed. After this analysis the concentration range of the nutrient variables were extended and a two level plus a star configuration factorial experimental design was performed. The experimental values are well represented by the linear and quadratic model equations obtained. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Factorial design; Acetic fermentation; Ethanol; Fermentation process. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662005000100011 Swine manure digestate treatment using electrocoagulation Scientia Agricola Mores,Rúbia; Kunz,Airton; Steffens,Juliana; Dallago,Rogério Marcos; Benazzi,Toni Luis; Amaral,André Cestonaro do. ABSTRACT Anaerobic biodigestion is an appropriate alternative for the treatment of swine wastewater due to its biogas generation properties and the possibility of its application as a source of energy for heating or electricity. However, digestate can still contain high levels of turbidity, organic carbon and nutrients and must be correctly managed as a biofertilizer, or treated to avoid any impact on the environment. Considering this, electrocoagulation (EC) shows promise as a technology because of its ease of handling and high efficiency in effluent remediation. This study aimed to evaluate the performance of EC in a batch system in the treatment of swine wastewater digestate. The wastewater used in the treatment was sampled from a 10 m3 biodigestor... Tipo: Info:eu-repo/semantics/article Palavras-chave: Electrode distance; Voltage; Factorial design; Batch system. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000500439 Registros recuperados: 17 Primeira ... 1 ... Última

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