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| Furet, Jean-pierre; Firmesse, Olivier; Gourmelon, Michele; Bridonneau, Chantal; Tap, Julien; Mondot, Stanislas; Dore, Joel; Corthier, Gerard. |
| Pollution of the environment by human and animal faecal pollution affects the safety of shellfish, drinking water and recreational beaches. To pinpoint the origin of contaminations, it is essential to define the differences between human microbiota and that of farm animals. A strategy based on real-time quantitative PCR (qPCR) assays was therefore developed and applied to compare the composition of intestinal microbiota of these two groups. Primers were designed to quantify the 16S rRNA gene from dominant and subdominant bacterial groups. TaqMan((R)) probes were defined for the qPCR technique used for dominant microbiota. Human faecal microbiota was compared with that of farm animals using faecal samples collected from rabbits, goats, horses, pigs, sheep... |
| Tipo: Text |
Palavras-chave: Quantitative PCR; Faecal microbiota; Human; Farm animals. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00000/11071/11297.pdf |
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| Zhao,Yumei; Tian,Gang; Chen,Daiwen; Zheng,Ping; Yu,Jie; He,Jun; Mao,Xiangbing; Yu,Bing. |
| ABSTRACT This experiment was conducted to evaluate the effects of dietary protein and net energy (NE) levels on growth performance, nutrient digestibility, nitrogen metabolism, and faecal microbiota of growing-finishing pigs. Eighteen crossed barrows were randomly allocated into one of three dietary treatments: high protein + high NE diet, low protein + high NE diet, and low protein + low NE diet. The whole experiment lasted 90 days and was divided into three phases (phase I: 25-50 kg; phase II: 50-75 kg; phase III: 75-105 kg). All pigs were individually housed in a metabolism cage and subjected to four-day total faeces and urine collection period at the end of each phase. There was no significant difference in growth performance, nutrient digestibility,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Faecal microbiota; Nitrogen metabolism; Nutrient digestibility; Pigs. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100516 |
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