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| Casablancas,Antoni; Cárdenas-Fernández,Max; Álvaro,Gregorio; Benaiges,Maria Dolors; Caminal,Glòria; Mas,Carles de; González,Glòria; López,Carmen; López-Santín,Josep. |
| Background: New enzymes for biotransformations can be obtained by different approaches including directed mutagenesis and in vitro evolution. These mutants have to be efficiently produced for laboratory research on bioreactions as well as for process development. In the framework of a European ERA-IB project, two different types of enzymes (ammonia lyases and aminotransferases) have been selected as biocatalysts for the synthesis of industrially relevant amines. New mutant enzymes have been obtained: a) aspartases able to recognize β-amino acids; b) ω-transaminases with improved activity. The objectives are to find out a common operational strategy applicable to different mutants expressed in E. coli with the same initial genetic... |
| Tipo: Journal article |
Palavras-chave: Amine transaminases; Ammonia lyases; E. coli; Fed-batch; Immobilized biocatalysts. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300004 |
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| Tonello,Tamiris U.; Andreani,Cristiane L.; Mari,Angelo G.; Fernandes,José R.; Gomes,Simone D.. |
| ABSTRACT The hydrogen is a fuel with a high energy density. Studies have sought to verify the potential of different residues and configurations of reactors in the production of biohydrogen. This study evaluated the influence of the organic loading rate on the production of biohydrogen from cassava starch wastewater (CSW) in anaerobic sequencing batch biofilm reactors (AnSBBR) operated in a fed-batch. In the tests, the organic loading rates of 9.0; 13.5 and 18.0 gCT L−1 d−1 were used, at cycle times (CT) of 4; 4 and 2 h (tests I, II and III, respectively). The reactor was inoculated with sludge from a pilot anaerobic reactor used in the treatment of CSW, heat treated (heated at 90°C for 15 min). With the increase of the organic load (condition I to II)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fed-batch; Fermentative process; Bioenergy; Cassava starch wastewater. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162018000500768 |
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