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| Queiroz,Kelly Marques; Silva,Maria-Luiza Martins; Prado,Nathália Duque; Lima,Paulo Marcelo Andrade; Diniz,Rosiane Dias Lopes; César,Isabela Costa; Pianetti,Gerson Antônio; Santos,Daniel Assis. |
| The development of a specific agar diffusion bioassay for the quantitative determination of fluconazole formulated in capsules was carried out using a strain of Candida albicans ATCC 18804 as the test organism. A prospective validation of the method showed adequate linearity (r²=0.9995), precision (R.S.D. = 4.0% for intra-day and 4.5% for inter-day precision) and accuracy (mean recovery = 102.9%). High performance liquid chromatography was chosen as a comparison method for the fluconazole determination. The contents of fluconazole determined by both methods, for four capsule samples, showed a strong correlation, confirmed by Pearson's correlation coefficient value (r = 0.9884). The bioassay is a suitable method for both research and pharmaceutical industry... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fluconazole/quantitative determination; Microbiological assay/agar diffusion; High performance liquid chromatography. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400012 |
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