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| Registros recuperados: 39 | |
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| Bao,Chengman; Tang,Xinhui; Ye,Shuming. |
| ABSTRACT The effect of pressure on the fluorescence of tryptophan in the presence of metal ions was studied by fluorescence spectrometry. It was found that at 60 MPa, the fluorescence intensity of M/Trp mixtures (M represented metal ions) increased compared to that at atmosphere pressure. The relative fluorescence efficiency of M/Trp mixtures increased with pressure. When the M/Trp ratio was above 10:1, the relative fluorescence efficiency in decreasing order was Cu2+/Trp mixtures, Ni2+/Trp mixtures and Mg2+ (K+)/Trp mixtures. When the ratio was below 10:1, the decreasing order was Cu2+/Trp mixtures and Ni2+ (Mg2+, K+)/Trp mixtures. The relative fluorescence efficiency increased with the concentration of Cu2+ and Ni2+. The variation was relate to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hydrostatic pressure; Tryptophan; Fluorescence; Metal ions. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100605 |
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| Bueno,Adeney de Freitas; Bueno,Regiane Cristina Oliveira de Freitas; Nabity,Paul David; Higley,Leon George; Fernandes,Odair Aparecido. |
| The twospotted spider mite Tetranychus urticae Koch is a common pest on soybean plants. To clarify plant-arthropod interaction on mite-soybean system, leaf fluorescence, photosynthetic responses to variable carbon dioxide levels, and chlorophyll content were evaluated. Significant photosynthetic rate reduction was observed due to stomatal limitation. Stomatal closure was the major plant physiological response. As a consequence, there was reduction in photosynthetic rates. Surprisingly, plants did not show chlorophyll content reduction associated with photosynthetic impairment. No differences in fluorescence data indicate that T. urticae injury did not impair the function of light harvesting and photoelectron transport. These results showed that T. urticae... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tetranychus urticae; Insect-plant relationship; Fluorescence; Gas exchange; Photosynthesis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400005 |
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| Avelar-Freitas,B.A.; Almeida,V.G.; Pinto,M.C.X.; Mourão,F.A.G.; Massensini,A.R.; Martins-Filho,O.A.; Rocha-Vieira,E.; Brito-Melo,G.E.A.. |
| Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the expertise of the analyst, can affect the results. We propose an alternative assay for evaluating cell viability that combines the TB exclusion test and the high sensitivity of the flow cytometry technique. Previous studies have demonstrated the ability of TB to emit fluorescence when complexed with proteins. According to our results, TB/bovine serum albumin and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypan blue; Fluorescence; Flow cytometry; Cytotoxicity. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400307 |
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| Torres Netto,Alena; Campostrini,Eliemar; Azevedo,Letícia C.; Souza,Marcelo A.; Ramalho,José C.; Chaves,Manuela M.. |
| Brazil is the largest world producer of papaya and the third largest exporter despite of only 1.5 to 2.0% of its production is exported. Such an underexplored exportation potential highlight the necessity for physiological studies on new cultivars and hybrids to verify their agronomic and commercial viability. Two Brazilian states, Bahia and Espirito Santo, are responsible for 80% of national production. Papaya can also be an agricultural alternative to north / northeast of Rio de Janeiro, because the region is close to consumer centers and have similar environmental conditions of the most productive regions. Nevertheless, it is worth to develop cultivars that can express the highest yield potential in this region. The aim of this work was to characterize... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya L.; Fluorescence; Gas exchange; Genotypes; Growth analyses. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000300005 |
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| Rocha,Andréa C.; Canal,Emerson C.; Campostrini,Eliemar; Reis,Fabrício O.; Cuzzuol,Geraldo Rogério F.. |
| Most studies of chromium have been realized in the sediments and in mangrove trees. However, little is known about its effect on growth and metabolism in this vegetation. L. racemosa plants were grown in solution with 20% of ionic strength of Hoagland and Arnon containing 100 mM NaCl and supplemented with 0.00, 0.05 and 0.50 mg L-1 of Cr+3. The growth, the pigments and gas exchange measurements showed no changes in response to doses of chromium. After 30 days the initiation of treatments, plants of L. racemosa had average 0.559 g of total dry weight, 1.34 mg L-1 of total chlorophyll and 7.9 µmol m-2 s-1 of A (CO2 photosynthetic assimilation). The photochemical potential efficiency was affected by the intermediate dose of 0.05 mg L-1 of Cr+3 (Fv/Fm=0.73)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carbohydrates; Fluorescence; Gas exchange; Growth; Heavy metal; Mangrove. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000200001 |
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| Zanella,Fábio; Watanabe,Tania Misae; Lima,Ana Lúcia da Silva; Schiavinato,Marlene Aparecida. |
| The effects of drought and rehydration on Canavalia ensiformis (L.) D.C. (jack bean) plants were evaluated using the following gas exchange parameters: net carbon assimilation rate (A), stomatal conductance (g s), Ci/Ca ratio and transpiration rate (E); chlorophyll a fluorescence: Fv/Fm and Fv/F0 ratio. The plants were cultivated under greenhouse conditions and after 30 days from the emergence, irrigation was suspended in the plants submitted to drought, to obtain the following predawn leaf water potential (psipd): -0.40 MPa (control), -1.00 MPa (moderate drought) and -2.30 Mpa (severe drought). Afterwards, the gas exchange and fluorescence analysis were initiated , and 24 h after rehydration the same analyses were repeated. The A, E, g s and Ci/Ca values... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Fluorescence; Moderate drought; Photosynthesis; Severe drought; Stomatal conductance. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000300008 |
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| González Aguilar, Susana. |
| Spathiphyllum spp. es conocida comúnmente como cuna de moisés, se utiliza como planta ornamental, presenta una hoja modificada (espata), de color blanco, y conforme madura enverdece. Este fenómeno típico de algunas Aráceas es poco conocido. En el presente trabajo se analizaron algunos parámetros que intervienen en el enverdecimiento. Para clasificar el enverdecimiento la clorofila se midió con un medidor portátil (SPAD-meter 50), se delimitaron tres etapas en unidades relativas “SPAD”; E1 con 5 a 10, E2 de 15 a 20 y E3 de 25 a 30, como referencia se usaron espatas blancas (B) y hojas del follaje (V) con un rango de 35 a 46 unidades relativas. El contenido de clorofila total en las hojas del follaje (2.99 gChlmg-1PF) fue el doble que en E3... |
| Tipo: Tesis |
Palavras-chave: Clorofila; Enverdecimiento; Fluorescencia; LSU de Rubisco; Maestría; Botánica; Chlorophyll; Greening; Fluorescence; LSU of Rubisco. |
| Ano: 2009 |
URL: http://hdl.handle.net/10521/1274 |
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| Финенко, З. З.; Чурилова, Т. Я.; Ли, Р. И.. |
| На основании обобщения большого количества данных, полученных в результате исследований многих авторов, установлены временные и региональные изменения вертикального распределения концентрации хлорофилла, флуоресценции. В холодный период (с декабря по март), когда вертикальное перемешивание поверхностного слоя происходит достаточно активно, концентрация хлорофилла в глубоководных районах моря распределена относительно равномерно в слое до 40 - 50 м. В теплый период (с мая по октябрь), после установления температурной стратификации в верхнем слое, преобладают профили с одним максимумом хлорофилла. Глубина расположения максимума хлорофилла меняется в широких пределах и зависит от его концентрации. Увеличение содержания хлорофилла в планктоне приводит к... |
| Tipo: Journal Contribution |
Palavras-chave: Черное море; Флуоресценция; Хлорофилл а; Фитопланктон; Биогенные вещества; Свет; The Black Sea; Chlorophyll a; Fluorescence; Phytoplankton; Nutrients; Irradiance Irradiance Nutrients (mineral) Phytoplankton Fluorescence Chlorophylls Fluorescence Phytoplankton Chlorophylls http://aims.fao.org/aos/agrovoc/c_10935 http://aims.fao.org/aos/agrovoc/c_13344 http://aims.fao.org/aos/agrovoc/c_1577. |
| Ano: 2005 |
URL: http://repository.ibss.org.ua/dspace/handle/99011/189 |
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| Tedetti, Marc; Bigot, Lionel; Turquet, Jean; Guigue, Catherine; Ferretto, Nicolas; Goutx, Madeleine; Cuet, Pascale. |
| We investigated the influence of freshwater discharges on biogeochemistry and benthic communities of La Saline fringing reef, the largest coral reef system of La Réunion Island (France, Indian Ocean). Sampling was conducted in February–March 2012 along a land-ocean continuum, including rivers, groundwater, reef waters, and oceanic waters. Our results indicated the discharges of basalt groundwater (enriched in nitrates) in the southern part of the reef, and the discharges of sand groundwater and river waters (enriched in organic matter) in sites located northward. These discharges mainly impacted fore reef surface waters, while groundwater resurgences were more diffuse and not clearly detected in depth. We highlighted the relationship Si(OH)4/NO3– to... |
| Tipo: Text |
Palavras-chave: Coral reefs; La Reunion; Indian Ocean; Eutrophication; Groundwater; Nutrients; Organic matter; Fluorescence. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00658/77030/78316.pdf |
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| Lund-hansen, Lc; Vang, T. |
| Upwelling of cold. saline, and nutrient rich water was observed in late September 1999 along an east-west transect in the SW Kattegat. The Kattegat forms part of the transitional zone between the high saline North Sea and the low saline Baltic Sea. The upwelling occurred after an extended period of northward flow and eastern winds in the Kattegat, that changed into a southward flow as wind ceased. The upwelling was the result of a combination of high current speeds and bottom topography whereby the high-speed inflow water was forced towards the surface at the slope. Nutrient data show that the upwelling brought nutrient rich bottom water to the light exposed surface and a related strong (1943.5 ml(-1)) bloom of Pseudo-nitzschia pseudodelicatissima was... |
| Tipo: Text |
Palavras-chave: Courant; Upwelling; Front; Fluorescence; Chlorophylle-a; Inflow; Upwelling; Frontal structure; Fluorescence; Chlorophyll-a. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00321/43249/42980.pdf |
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| Younes, Wan; Bensoussan, N; Romano, Jc; Arlhac, D; Lafont, Maxime. |
| Between 1996 and 2000, 79 cruises have been achieved following always the same transect between Marseilles and the Rhone river's mouth. While cruising, salinity, temperature and fluorescence of surface waters (1.5 m) were recorded every minute, in association with precise GPS position. The whole set of data. (i.e. 128 measured points per cruise) constitutes the Sorcom series whose synthetic results ate presented here. Despite the high spatial and temporal. variability encountered between two successive cruises, the regularity and duration of the sampling strategy permitted to define coherent spatial zonation and temporal evolutions. In the Cape Couronne vicinity the dynamic of the eastern extension of the dilution plume clearly changes. Westward, waters... |
| Tipo: Text |
Palavras-chave: Rhône; Panache de dilution; Observation à long terme; Fluorescence; Salinité; Rhone river; Dilution plume; Long term observation; Fluorescence; Salinity. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00321/43260/42991.pdf |
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| Lefebvre, Alain; Poisson-caillault, Emilie; Grassi, Kelly. |
| Installée dans la rade de Boulogne-sur-Mer et inaugurée le 25 novembre 2004, la station MAREL Carnot mesure, toutes les 20 minutes, la salinité, la température de l’eau et de l’air, la fluorescence, la turbidité, la concentration en oxygène dissous, le pourcentage de saturation en oxygène, le P.A.R., l’humidité relative, la direction et la vitesse du vent, la hauteur d’eau et, toutes les 12 heures, la concentration en nitrate, en phosphate et en silicium. Ce rapport vise à présenter les principaux éléments utiles à l’utilisateur des données afin de pouvoir adapter son étude en fonction de la disponibilité des données, de leur qualité et, bien entendu, de son objectif. Les résultats des paramètres fluorescence, turbidité, concentration en oxygène,... |
| Tipo: Text |
Palavras-chave: Manche orientale; Boulogne-sur-Mer; Haute fréquence temporelle; MAREL; Hydrologie; Fluorescence. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00441/55258/56751.pdf |
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| Rivera-ingraham, Georgina A.; Rocchetta, Iara; Bickmeyer, Ulf; Meyer, Stefanie; Abele, Doris. |
| Background: Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes at cellular level. Results: Together with oxidative stress measurements, here we report application of LIT to bivalve gills for ex-vivo analysis of gill physiology and mapping of ROS and RNS formation in the living tissue. Our results indicate that a) mitochondria located in the basal parts of the epithelial cells close to the blood vessels are hyperpolarized with high Delta Psi m, whereas... |
| Tipo: Text |
Palavras-chave: Bivalve; Gill; Live-imaging; Fluorescence; Mitochondria; ROS; RNS. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00626/73808/74412.pdf |
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| Retho, Michael. |
| Ce rapport présente les éléments qui ont conduit à la qualification des données acquises en 2018 et 2019 par la station de mesure haute fréquence MOLIT (Mer Ouverte Littoral). La bouée MOLIT est déployée depuis 2008 en baie de Vilaine, une des zones de la côte atlantique les plus menacées par l’eutrophisation. Cette bouée instrumentée constitue un des systèmes de mesure du réseau de mesure haute fréquence COAST-HF (Coastal OceAn observing SysTem – High Frequency). Cette station est équipée d’une sonde multiparamètres qui mesure les paramètres température, salinité, oxygène dissous, turbidité et fluorescence. Les mesures sont effectuées à deux niveaux (surface et fond) avec une fréquence horaire. Ce rapport synthétise les informations qui ont permis de... |
| Tipo: Text |
Palavras-chave: MOLIT; Bouée instrumentée; Haute fréquence; Baie de Vilaine; Qualification; Température; Salinité; Oxygène dissous; Turbidité; Fluorescence. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00636/74842/75193.pdf |
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| Salin, Karine; Villasevil, Eugenia M.; Auer, Sonya K.; Anderson, Graeme J.; Selman, Colin; Metcalfe, Neil B.; Chinopoulos, Christos. |
| The use of tissue homogenate has greatly aided the study of the functioning of mitochondria. However, the amount of ATP produced per oxygen molecule consumed, that is, the effective P/O ratio, has never been measured directly in tissue homogenate. Here we combine and refine existing methods previously used in permeabilized cells and isolated mitochondria to simultaneously measure mitochondrial ATP production (JATP) and oxygen consumption (JO(2)) in tissue homogenate. A major improvement over existing methods is in the control of ATPases that otherwise interfere with the ATP assay: our modified technique facilitates simultaneous measurement of the rates of uncorrected ATP synthesis and of ATP hydrolysis, thus minimizing the amount of tissue and time needed.... |
| Tipo: Text |
Palavras-chave: ATPase; Fluorescence; Magnesium green; Oxidative phosphorylation; Oxygraph. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00414/52500/53300.pdf |
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| Rumin, Judith; Bonnefond, Hubert; Saint-jean, Bruno; Rouxel, Catherine; Sciandra, Antoine; Bernard, Olivier; Cadoret, Jean-paul; Bougaran, Gael. |
| Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. In addition, they do not compete for arable land and offer very high lipid yield potential. Major challenges for the development of this resource are to select lipid-rich strains using high-throughput staining for neutral lipid content in microalgae species. For this purpose, the fluorescent dyes most commonly used to quantify lipids are Nile red and BODIPY 505/515. Their fluorescent staining for lipids offers a rapid and inexpensive analysis tool to measure neutral lipid content,... |
| Tipo: Text |
Palavras-chave: Nile red; BODIPY 505/515; Microalgae; Neutral lipid; Fluorescence; Biodiesel. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00257/36823/35451.pdf |
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| Registros recuperados: 39 | |
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