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Freezing and chemical preservatives alter the stable isotope values of carbon and nitrogen of the Asiatic clam (Corbicula fluminea) ArchiMer
Syvaeranta, Jari; Martino, Aurelia; Kopp, Dorothee; Cereghino, Regis; Santoul, Frederic.
We tested the impacts of most common sample preservation methods used for aquatic sample materials on the stable isotope ratios of carbon and nitrogen in clams, a typical baseline indicator organism for many aquatic food web studies utilising stable isotope analysis (SIA). In addition to common chemical preservatives ethanol and formalin, we also assessed the potential impacts of freezing on delta C-13 and delta N-15 values and compared the preserved samples against freshly dried and analysed samples. All preservation methods, including freezing, had significant impacts on delta C-13 and delta N-15 values and the effects in general were greater on the carbon isotope values (1.3-2.2aEuro degrees difference) than on the nitrogen isotope values (0.9-1.0aEuro...
Tipo: Text Palavras-chave: Formalin; Freezing; Ethanol; Preservation; Stable isotope analysis.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00021/13192/10271.pdf
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Antinociception synergy between the peripheral and spinal sites of the heme oxygenase-carbon monoxide pathway BJMBR
Nascimento,C.G.O.; Branco,L.G.S..
We have shown that the peripheral and spinal cord heme oxygenase (HO)-carbon monoxide (CO)-soluble guanylate cyclase-cGMP pathways play an important role in antinociception in the rat experimental formalin model. Our objective was to determine if there is synergism between peripheral (paw) and spinal HO-CO pathways in nociception. Rats were handled and adapted to the experimental environment for a few days before the formalin test, in which 50 µL of a 1% formalin was injected subcutaneously into the dorsal surface of the right hind paw. The animals were then observed for 1 h and the frequency of flinching behavior was taken to represent the nociceptive response. Thirty minutes before the test, rats were pretreated with intrathecal injections of the HO...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nociception; Carbon monoxide; Formalin; Hypernociception; Antinociception.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100020
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