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| Mata-Miranda,Monica Maribel; Vazquez-Zapien,Gustavo Jesus; Rojas-Lopez,Marlon; Sanchez-Monroy,Virginia; Perez-Ishiwara,David Guillermo; Delgado-Macuil,Raul Jacobo. |
| Abstract Background Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. Results In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed... |
| Tipo: Journal article |
Palavras-chave: Pluripotent stem cells; Differentiated kidney cells; Vibrational spectroscopy; Fourier transform infrared; Principal components analysis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100207 |
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| Castellane,Tereza Cristina Luque; Otoboni,Alda Maria Machado Bueno; Lemos,Eliana Gertrudes de Macedo. |
| ABSTRACT Increasing attention has been given, over the past decades, to the production of exopolysaccharides (EPS) from rhizobia, due to their various biotechnological applications. Overall characterization of biopolymers involves evaluation of their chemical, physical, and biological properties; this evaluation is a key factor in understanding their behavior in different environments, which enables researchers to foresee their potential applications. Our focus was to study the EPS produced by Mesorhizobium huakuii LMG14107, M. loti LMG6125, M. plurifarium LMG11892,Rhizobium giardini bv. giardiniH152T, R. mongolense LMG19141, andSinorhizobium (= Ensifer)kostiense LMG19227 in a RDM medium with glycerol as a carbon source. These biopolymers were isolated and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acidic exopolysaccharide; Fourier transform infrared; Microbial heteropolysaccharides; Rhizobium; Sinorhizobium. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000601566 |
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