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| Pilatti,RM; Furian,TQ; Lima,DA; Finkler,F; Brito,BG; Salle,CTP; Moraes,HLS. |
| ABSTRACT Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fowl cholera; Inoculation; Pasteurella multocida; Pathogenicity index. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200255 |
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| Almeida,C.N.; Furian,T.Q.; Borges,K.A.; Perdoncini,G.; Mauel,M.J.; Rocha,S.L.S.; Nascimento,V.P.; Salle,C.T.P.; Moraes,H.L.S.. |
| ABSTRACT Fowl Cholera (FC) is a disease caused by Pasteurella multocida. The severity of this disease is partly caused by virulence factors. Genes encoding fimbriae, capsule, sialidases and proteins for iron metabolism may be related to P. multocida’s ability to infect the host. Besides to examining DNA for the presence of virulence genes, DNA is essential for the diagnostic and FTA cards are an alternative for genetic material transport. The study aims to evaluate the viability of P. multocida DNA transport using the cards and to detect 14 virulence genes in 27 strains isolated from FC cases in the United States by multiplex-PCR. No growth was observed in any of the FTA cards, which was essential to assess the security. Furthermore, DNA detection was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fowl cholera; FTA cards; Virulence genes. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601855 |
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