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	Provedor de dados Arq. Bras. Med. Vet. Zootec. (1) Genet. Mol. Biol. (1) Autor Arruga,M.V. (1) Denison,Stacy R. (1) Greenbaum,Ira F. (1) Llambí,S. (1) Multani,Asha S. (1) Pathak,Sen (1) Mais... Palavra-chave Fragile sites (2) Aphidicolin (1) Cattle (1) DOP-PCR (1) Heritability (1) Microdissection (1) Rob(14q21q) (1) Robertsonian translocation (1) X sexual chromosome (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2008 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Fragility in the 14q21q translocation region Genet. Mol. Biol. Denison,Stacy R.; Multani,Asha S.; Pathak,Sen; Greenbaum,Ira F.. Aphidicolin (APC)-induced chromosomal breakage was analyzed for women representing three generations of a single family and carrying a Robertsonian translocation rob(14q21q). Fluorescence in situ hybridization (FISH) analysis confirmed the dicentric constitution of the derived chromosome and indicated the absence of beta-satellite signal at the translocation region. Per-individual analysis of metaphases from APC-treated peripheral blood lymphocyte cultures identified significantly nonrandom chromosomal breakage at the translocation region in all three individuals examined. The APC-inducible fragility at the 14q21q translocation region suggests that this rearrangement was the result of chromosomal mutation at fragile site(s) in the progenitor chromosomes,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rob(14q21q); Robertsonian translocation; Fragile sites; Aphidicolin; Heritability. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300003 Molecular approach of the fragile chromosomal region Xq31-34 in cattle (Bos taurus) by microdissection and DOP-PCR Arq. Bras. Med. Vet. Zootec. Llambí,S.; Arruga,M.V.. Fragile sites (FS) are chromosomal regions where the normal compactation of chromatine is not observed. FRAXA (Fra Xq27.3, X sexual chromosome) is one of the most studied FS in humans. FRAXA is an expansion of the trinucleotide CGG located in the gene FMR-1. In cattle, sites of chromosomal fragility were reported in BTAX, associated with different pathologies and fertility impairment. Chromosomal microdissection has became a valuable tool for isolating chromatine fragments. In this work, it was combined the chromosomal microdissection technique with DOP-PCR in order to carry out a molecular analysis of the fragile chromosomal region BTAXq31-34. In that region, polymorphic DNA-RAPD sequences (GC rich) are present and sequences of the gene FMR-1 are missing.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Fragile sites; DOP-PCR; Microdissection; X sexual chromosome. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400023 Registros recuperados: 2 Primeira ... 1 ... Última

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