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Cryopreserved sperm for oocyte fertilization of dourado Salminus brasiliensis 65
Weingartner,Marcos; Zanandrea,Ana Carolina Volpato; Zaniboni Filho,Evoy.
Salminus brasiliensis is a migratory fish that has attracted considerable interest for aquaculture. Several procedures for induced spawning are known; however, there is a lack of protocol which enables the use of cryopreserved semen. Therefore, this study was conducted to investigate the use of cryopreserved semen using different volumes of cryopreserved semen relative to oocytes, different activators solutions and different maintenance time during the fertilization of dourado to evaluate the impact of these parameters on the fertilization rate. The semen was collected, cryopreserved in 0.5mL straws and stored in a dry shipper. Oocytes samples were fertilized according to each treatment. The different activator solutions and the contact times of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Frozen semen; Gamete ratio; Semen activation; Activator solution.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500892
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Factors Affecting the Fertility of Ewes after Intrauterine Insemination with Frozen-Thawed Semen During the Non-Breeding Season 19
FUKUI, Yutaka; KOHNO, Hirohide; OKABE, Kentaro; KATSUKI, Sara; YOSHIZAWA, Masahiro; TOGARI, Tetsuro; WATANABE, Hiroyuki; 福井, 豊.
In this study, two successive field trials were conducted during the non-breeding season to investigate various factors affecting on fertility of Suffolk ewes after intrauterine insemination with frozen-thawed semen. In the first year (Experiment 1), three sperm numbers per insemination dose (0.25, 0.5 and 1 million sperm) and five sheep farms were used, and in the second year (Experiment 2), parity, age, body weight, body condition score (BCS) and postpartum days were investigated to compare pregnancy and lambing rates. High pregnancy and lambing rates (70.6 and 70.6%, respectively) were obtained with 0.25 million sperm per dose. There were no significant differences in the pregnancy and lambing rates among the five farms, but there was a tendency for one...
Palavras-chave: Artificial insemination; Fertility; Frozen semen; Sheep.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2817
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