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	Provedor de dados BJMBR (1) BJM (1) Autor Buckeridge,M.S. (1) Castillo,Boris Gutarra (1) Fernández,Rubén Cuervo (1) Figueiredo-Ribeiro,R.C.L. (1) Itaya,N.M. (1) Maiorano,Alfredo Eduardo (1) Maresma,Beatriz Guilarte (1) Rodrigues,Maria Filomena de Andrade (1) Silva,Elda Sabino da (1) Mais... Palavra-chave Fructosyltransferase (2) Aspergillus oryzae (1) Asteraceae (1) Fructan biosynthesis (1) Fructooligosaccharides (1) Inulin (1) Mutagenesis (1) UV irradiation (1) Viguiera discolor (1) Β-fructofuranosidase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (1) 1999 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae) BJMBR Itaya,N.M.; Figueiredo-Ribeiro,R.C.L.; Buckeridge,M.S.. Sucrose:sucrose fructosyltransferase (SST) and fructan:fructan fructosyl-transferase (FFT) activities from crude extracts of tuberous roots of Viguiera discolor growing in a preserved area of cerrado were analyzed in 1995-1996. SST activity was characterized by the synthesis of 1-kestose from sucrose and FFT activity by the production of nystose from 1-kestose. The highest fructan-synthesizing activity was observed during early dormancy (autumn), when both (SST and FFT) activities were high. The increase in synthetic activity seemed to start during the fruiting phase in the summer, when SST activity was higher than in spring. During winter and at the beginning of sprouting, both activities declined. The in vitro synthesis of high molecular mass fructans... Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulin; Fructosyltransferase; Fructan biosynthesis; Viguiera discolor; Asteraceae. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400010 Mutagenesis of Aspergillus oryzae IPT-301 to improve the production of β-fructofuranosidase BJM Maresma,Beatriz Guilarte; Castillo,Boris Gutarra; Fernández,Rubén Cuervo; Silva,Elda Sabino da; Maiorano,Alfredo Eduardo; Rodrigues,Maria Filomena de Andrade. Aspergillus oryzae IPT-301, previously reported as a β-fructofuranosidase producing microorganism, was successfully mutated using UV irradiation at 253.7 nm followed by the screening of survivors resistant to certain stress conditions. Strains were first subjected to the β-fructofuranosidase activity assay using a portion from the colony grown in Petri dish as the enzyme source. Seven mutants with fructofuranosidase activity values relative to the parent culture between 140 -190% were selected from survivors grown at temperature of 40ºC or 0.018% (w/v) sodium dodecyl sulfate concentration. They were cultivated on a rotary shaker to characterize mycelium and extracellular fructosyltransferase activities. Three mutants named IPT-745, IPT-746 and IPT-748... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus oryzae; Mutagenesis; Β-fructofuranosidase; Fructosyltransferase; Fructooligosaccharides; UV irradiation. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100027 Registros recuperados: 2 Primeira ... 1 ... Última

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