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Homrich,Milena Schenkel; Wiebke-Strohm,Beatriz; Weber,Ricardo Luís Mayer; Bodanese-Zanettini,Maria Helena. |
Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transgenic plants; Particle bombardment; Agrobacterium systems; Functional analysis; Genetic improvement. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015 |
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Moreira,Fernanda da S.; Costa,Pedro B. da; Souza,Rocheli de; Beneduzi,Anelise; Lisboa,Bruno B.; Vargas,Luciano K.; Passaglia,Luciane M. P.. |
Abstract In the pursuit of sustainable agriculture, bioinoculants usage as providers of a crop's needs is a method to limit environmental damage. In this study, a collection of cultivable putative plant growth promoting (PGP) bacteria associated with wheat crops was obtained and this bacterial sample was characterized in relation to the functional diversity of certain PGP features. The isolates were obtained through classical cultivation methods, identified by partial 16S rRNA gene sequencing and characterized for PGP traits of interest. Functional diversity characterization was performed using Categorical Principal Component Analysis (CatPCA) and Multiple Correspondence Analysis (MCA). The most abundant genera found among the 346 isolates were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant growth promoting bacteria; Categorical Principal Component Analysis; Functional analysis; Burkholderia genus. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100111 |
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Bittner, Lucie; Halary, Sebastien; Payri, Claude; Cruaud, Corinne; De Reviers, Bruno; Lopez, Philippe; Bapteste, Eric. |
Background: Improving knowledge of biodiversity will benefit conservation biology, enhance bioremediation studies, and could lead to new medical treatments. However there is no standard approach to estimate and to compare the diversity of different environments, or to study its past, and possibly, future evolution. Presentation of the hypothesis: We argue that there are two conditions for significant progress in the identification and quantification of biodiversity. First, integrative metagenomic studies - aiming at the simultaneous examination (or even better at the integration) of observations about the elements, functions and evolutionary processes captured by the massive sequencing of multiple markers - should be preferred over DNA barcoding projects... |
Tipo: Text |
Palavras-chave: Microbial diversity; Species concept; Dna barcodes; Ecological perspective; Community; Functional analysis; Rhodophyta; Systematics; Fragments; Taxonomy. |
Ano: 2010 |
URL: https://archimer.ifremer.fr/doc/00202/31321/29733.pdf |
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Yuliet Morera Darias. |
El desarrollo alcanzado en la camaronicultura hasta el presente exige estudios cada vez más novedosos en vista a enfrentar nuevos retos. En este sentido resulta necesario el conocimiento de la estructura-función de los neuropéptidos de la familia de la Hormona Hiperglucemiante de Crustáceos (familia HHC). Ella juega un importante papel en el metabolismo y la fisiología influyendo directamente sobre la muda, el crecimiento, la reproducción, el metabolismo de la glucosa y lípidos, entre otras. La Hormona Hiperglucemiante de Crustáceos (HHC) es el componente mayoritario de la familia HHC y el único neuropéptido aislado y secuenciado hasta el presente. Ella es una hormona pleiotrópica y su función principal es regular el nivel de glucosa en la... |
Tipo: Theses and Dissertations |
Palavras-chave: Marine shrimp culture; Anatomical structures; Functional analysis. |
Ano: 2009 |
URL: http://hdl.handle.net/1834/5528 |
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García-Salcedo,Ángela Janet; Torres-Vargas,Olga Lucía; Ariza-Calderón,Hernando. |
Abstract Quinoa, amaranth, and chia flours have considerable amounts of protein, starch, dietary fiber, lipids, minerals, vitamins, and bioactive components, which provide in them exceptional properties for human nutrition. The aim of this research was the physical-chemical characterization of quinoa, amaranth, and chia flours and seeds through proximal, optical, functional, thermal, and structural analyses. From the results obtained during the proximal analysis, considerable proportions were determined of protein and fiber for the three flours, with the highest values in chia flour at 28.56% and 39.8%, respectively. These results were corroborated by optical microscopy analysis performed on longitudinal cuts in the three types of seeds. Thermal parameters... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioactive components; Functional analysis; Protein alternatives; Starch; Structural characterization. |
Ano: 2018 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000200215 |
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Wu,Xiaofen; Ruan,Lei; Yang,Yi; Mei,Qi. |
Abstract Background This study aimed to investigate the gene expression changes associated with carcinoma-associated fibroblasts (CAFs) involving in non-small cell lung carcinoma (NSCLC). Methods We downloaded the GEO series GSE22862, which contained matched gene expression values for 15 CAF and normal fibroblasts samples, and series GSE27289 containing SNP genotyping for four matched NSCLC samples. The differentially expressed genes in CAF samples were identified using the limma package in R. Then we performed gene ontology (GO) and pathway enrichment analysis and protein–protein interaction (PPI) network construction using the identified DEGs. Moreover, aberrant cell fraction, ploidy, allele-specific copy number, and loss of heterozygosity... |
Tipo: Journal article |
Palavras-chave: Carcinoma-associated fibroblast; Non-small cell lung carcinoma; Differentially expressed genes; Allele-specific copy number; Functional analysis. |
Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100204 |
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