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Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens J. Venom. Anim. Toxins incl. Trop. Dis.
Richini-Pereira,VB; Bosco,SMG; Theodoro,RC; Barrozo,L; Bagagli,E.
Road-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Road-killed animals; Fungal pathogens; Eco-epidemiology; PCR.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400011
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A re-appraisal of Harknessia (Diaporthales), and the introduction of Harknessiaceae fam. nov. Naturalis
Crous, P.W.; Summerell, B.A.; Shivas, R.G.; Carnegie, A.J.; Groenewald, J.Z..
Harknessiaceae is introduced as a new family in the ascomycete order Diaporthales to accommodate species of Harknessia with their Wuestneia-like teleomorphs. The family is distinguished by having pycnidial conidiomata with brown, furfuraceous margins, brown conidia with hyaline, tube-like basal appendages, longitudinal striations, and rhexolytic secession. Six species occurring on Eucalyptus are newly introduced, namely H. australiensis, H. ellipsoidea, H. pseudohawaiiensis, and H. ravenstreetina from Australia, H. kleinzeeina from South Africa, and H. viterboensis from Italy. Epitypes are designated for H. spermatoidea and H. weresubiae, both also occurring on Eucalyptus. Members of Harknessia are commonly associated with leaf spots, but also occur as...
Tipo: Article / Letter to the editor Palavras-chave: Biodiversity; Fungal pathogens; Harknessiaceae; ITS; LSU; Phylogeny; Systematics.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/532250
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Fungal pathogens of Proteaceae Naturalis
Crous, P.W.; Summerell, B.A.; Swart, L.; Denman, S.; Taylor, J.E.; Bezuidenhout, C.M.; Palm, M.E.; Marincowitz, S.; Groenewald, J.Z..
Species of Leucadendron, Leucospermum and Protea (Proteaceae) are in high demand for the international floriculture market due to their brightly coloured and textured flowers or bracts. Fungal pathogens, however, create a serious problem in cultivating flawless blooms. The aim of the present study was to characterise several of these pathogens using morphology, culture characteristics, and DNA sequence data of the rRNA-ITS and LSU genes. In some cases additional genes such as TEF 1-α and CHS were also sequenced. Based on the results of this study, several novel species and genera are described. Brunneosphaerella leaf blight is shown to be caused by three species, namely B. jonkershoekensis on Protea repens, B. nitidae sp. nov. on Protea nitida and B....
Tipo: Article / Letter to the editor Palavras-chave: Biodiversity; Cut-flower industry; Fungal pathogens; ITS; LSU; Phylogeny; Systematics.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/532487
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Reappraisal of the genus Alternariaster (Dothideomycetes) Naturalis
Alves, J.L.; Woudenberg, J.H.C.; Duarte, L.L.; Crous, P.W.; Barreto, R.W..
Alternariaster was erected in 2007 to accommodate Alternaria helianthi, a fungal species known to cause leaf spots on Helianthus annuus (sunflower). It was segregated from Alternaria based on conidial morphology. Recently an unknown alternaria-like dematiaceous fungus was found associated with leaf spots on Bidens sulphurea (yellow cosmos) in Brazil. Based on a multi-gene phylogeny of parts of the ITS and LSU genes, this fungus was placed within the Leptosphaeriaceae with Alternariaster helianthi as its closest neighbour. Additional genes sequenced, RPB2 and GAPDH, confirmed this close relationship. The fungus on B. sulphurea has smaller conidia, 50–97.5 × 12.5–20 μm, compared to Al. helianthi, 80–160 × 18–30 μm, and lacks oblique or transverse septa which...
Tipo: Article / Letter to the editor Palavras-chave: Alternaria; Fungal pathogens; Host-range; Multi-gene phylogeny.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/531881
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Structure and biological functions of fungal cerebrosides Anais da ABC (AABC)
Barreto-Bergter,Eliana; Pinto,Marcia R.; Rodrigues,Marcio L..
Ceramide monohexosides (CMHs, cerebrosides) are glycosphingolipids composed of a hydrophobic ceramide linked to one sugar unit. In fungal cells, CMHs are very conserved molecules consisting of a ceramide moiety containing 9-methyl-4,8-sphingadienine in amidic linkage to 2-hydroxyoctadecanoic or 2-hydroxyhexadecanoic acids, and a carbohydrate portion consisting of one residue of glucose or galactose. 9-Methyl 4,8-sphingadienine-containing ceramides are usually glycosylated to form fungal cerebrosides, but the recent description of a ceramide dihexoside (CDH) presenting phytosphingosine in Magnaporthe grisea suggests the existence of alternative pathways of ceramide glycosylation in fungal cells. Along with their unique structural characteristics, fungal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucosylceramide; Cerebrosides; Glycosphingolipids; Fungal pathogens; Antifungal therapy.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000100007
Registros recuperados: 5
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