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	Provedor de dados BJM (2) Autor Carvalho,Patricia O. (1) Fregolente,Leonardo V. (1) Fregolente,Patricia B.L. (1) Lele,S.S. (1) Maciel,Maria R.W. (1) Ronda,Srinivasa Reddy (1) Mais... Palavra-chave Gamma linolenic acid (2) Borage oil (1) Geotrichum candidum (1) Light intensity (1) Lipase (1) Oil supplementation (1) Temperature (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2009 (1) 2008 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Culture Conditions stimulating high γ-Linolenic Acid accumulation by Spirulina platensis BJM Ronda,Srinivasa Reddy; Lele,S.S.. Gamma-linolenic acid (GLA) production by Spirulina platensis under different stress-inducing conditions was studied. Submerged culture studies showed that low temperature (25ºC), strong light intensity (6 klux) and primrose oil supplement (0.8%w/v) induced 13.2 mg/g, 14.6 mg/g and 13.5 mg linolenic acid per gram dry cell weight respectively. A careful observation of fatty acid profile of the cyanobacteria shows that, oleic acid and linoleic acid, in experiments with varying growth temperature and oil supplements respectively, helped in accumulating excess γ-linolenic acid. In addition, cultures grown at increasing light regimes maintained the γ-linolenic acid to the total fatty acid ratio(GLA/TFA) constant, despite any change in γ-linolenic acid content of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gamma linolenic acid; Light intensity; Oil supplementation; Temperature. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400018 Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration BJM Fregolente,Patricia B.L.; Fregolente,Leonardo V.; Maciel,Maria R.W.; Carvalho,Patricia O.. Gamma-linolenic acid (GLA, 18:3, cis- 6,9,12-octadecatrienoic acid), an important compound in n-6 eicosanoid family biosynthesis, occurs in the lipids of a few plant and microbial sources. This study focused on the screening of microbial strains with suitable lipase activity for enrichment of GLA by selective hydrolysis of the borage oil (21.6 % of GLA/total fatty acids). Firstly, 352 microrganisms were tested for their lipolytic capacity using screening techniques on agar plates containing borage oil, strains were then selected and screened for their activity (U/mg) using both submerged fermentation (SmF) and solid state fermentation (SSF). The rate of hydrolysis and the selective preference of these hydrolytic enzymes towards fatty acids, with a special... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gamma linolenic acid; Borage oil; Lipase; Geotrichum candidum. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400004 Registros recuperados: 2 Primeira ... 1 ... Última

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