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	Provedor de dados BABT (1) BJMBR (1) Autor AK,Sahoo (1) Andrade,A.S.R. (1) KP,Sure (1) PB,Dandge (1) PK,Bhagwat (1) PV,Kotnis (1) RC,Ranveer (1) Tunes,H. (1) Vilela,L. (1) Mais... Palavra-chave Gel filtration (2) Antimicrobial activity (1) Bacteriocin (1) Glucagon (1) Insulin (1) Ion-exchange chromatography (1) Lactobacillus viridescence (1) NICM 2167 (1) Protein purification (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2016 (1) 1997 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Production and Characterization of Bacteriocin Produced by Lactobacillus Viridescence(NICM 2167) BABT KP,Sure; PV,Kotnis; PK,Bhagwat; RC,Ranveer; PB,Dandge; AK,Sahoo. The present study focused on the production optimization of bacteriocin by Lactobacillus viridescence NICM 2167 followed by its purification and characterization. The bacteriocins are antimicrobial peptides produced by many Gram positive and Gram negative bacteria.The bacteriocin produced by LAB (lactic acid bacteria) received attention in recent years due to their potential application as natural preservatives in food. Bacteriocinproduced by Lactobacillus viridescence showed broad range of antimicrobial activity against food borne pathogens. Production parameters were optimized showing highest production of bacteriocinin MRS broth with pH= 7.0 incubated at 37°C for 48 h. Bacteriocin was purified in two steps involving ammonium sulphate precipitation... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus viridescence; NICM 2167; Bacteriocin; Antimicrobial activity; Gel filtration. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100407 Purification of bovine pancreatic glucagon as a by-product of insulin production BJMBR Andrade,A.S.R.; Vilela,L.; Tunes,H.. A process for purifying bovine pancreatic glucagon as a by-product of insulin production is described. The glucagon-containing supernatant from the alkaline crystallization of insulin was precipitated using ammonium sulfate and isoelectric precipitation. The isoelectric precipitate containing glucagon was then purified by ion-exchange chromatography on Q-Sepharose FF, gel filtration on Sephadex G-25 and ion-exchange chromatography on S-Sepharose FF. A pilot scale test was performed with a recovery of 87.6% and a purification factor of 8.78 for the first chromatographic step, a recovery of 75.1% and a purification factor of 3.90 for the second, and a recovery of 76.2% and a purification factor of 2.36 for the last one. The overall yield was 50%, a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucagon; Protein purification; Gel filtration; Ion-exchange chromatography; Insulin. Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200006 Registros recuperados: 2 Primeira ... 1 ... Última

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