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| Registros recuperados: 61 | |
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| PANTIC,IGOR; PANTIC,SENKA; PAUNOVIC,JOVANA; PEROVIC,MILAN. |
| Grey level co-occurrence matrix analysis (GLCM) is a well-known mathematical method for quantification of cell and tissue textural properties, such as homogeneity, complexity and level of disorder. Recently, it was demonstrated that this method is capable of evaluating fine structural changes in nuclear structure that otherwise are undetectable during standard microscopy analysis. In this article, we present the results indicating that entropy, angular second moment, variance, and texture correlation of lymphocyte nuclear structure determined by GLCM method are different in thymus cortex when compared to medulla. A total of 300 thymus lymphocyte nuclei from 10 one-month-old mice were analyzed: 150 nuclei from cortex and 150 nuclei from medullar regions of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Texture analysis; GLCM matrix; CD4; CD8; Gene. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301063 |
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| Ropert,C.. |
| Gene therapy is an active field that has progressed rapidly into clinical trials in a relatively short time. The key to success for any gene therapy strategy is to design a vector able to serve as a safe and efficient gene delivery vehicle. This has encouraged the development of nonviral DNA-mediated gene transfer techniques such as liposomes. Many liposome-based DNA delivery systems have been described, including molecular components for targeting given cell surface receptors or for escaping from the lysosomal compartment. Another recent technology using cationic lipids has been evaluated and has generated substantial interest in this approach to gene transfer. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene; PH-sensitive liposome; Cationic lipid; Transfection. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200004 |
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| Natalini,Cláudio Corrêa; Linardi,Renata Lehn. |
| P-glycoprotein (P-gp) is a membrane transporter encoded in the Multi-drug Resistance (MDR1) gene expressed in several normal tissues and over expressed in tumor cells. P-gp was already identified in different species but not yet in equine. MDR1 gene and P-gp are able to interfere with bioavailability and disposition of several drugs, altering pharmacokinetic and pharmacodinamic of drugs. The presence of the MDR1 and P-gp in the central nervous system blocks the entry of certain drugs in this tissue and reduces drug absorption and enhances drug elimination when P-gp and MDR1 are presented in the gastrointestinal tract. This study showed that the MDR1 gene is present in equine ileum. Future studies on the impact of the P-glycoprotein encoded gene MDR1 on... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular biology; Opioids; Equine; Gene; MDR1; ABCB1. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100048 |
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| Huang,Hua-Tuo; Guo,Jing; Xiang,Yang; Chen,Jian-Ming; Luo,Hong-Cheng; Meng,Lan-Qing; Wei,Ye-Sheng. |
| Abstract Cluster of differentiation 40 (CD40), the receptor for CD154, is a member of the tumor necrosis factor (TNF) receptor superfamily. Several studies have been conducted to investigate the effect of the CD40 rs1883832 polymorphism on atherosclerotic disease in different population; however, inconsistent results were obtained. In this study, we investigated the association of four polymorphisms (rs1883832, rs13040307, rs752118 and rs3765459) of CD40 gene and their effect on CD40 expression with the risk of ischemic stroke (IS) in a Chinese population. Three hundred and eighty patients with IS and 450 control subjects were included in the study. The CD40 polymorphisms were discriminated by Snapshot SNP genotyping assay. Serum soluble CD40 (sCD40)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CD40; Gene; Polymorphism; Ischemic stroke. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300442 |
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| Amoli,Mahsa M.; Amiri,Parvin; Tavakkoly-Bazzaz,Javad; Charmchi,Elham; Hafeziyeh,Jila; Keramatipour,Mohammad; Abiri,Maryam; Ranjbar,Shirin Hasani; Larijani,Bagher. |
| The transcription factor 7-like 2 gene (TCF7L2) rs7903146 T allele is constantly associated with Type 2 diabetes in various populations and ethnic groups. Nevertheless, this has not been observed in two studies involving Arab populations. The aim of the present study was to investigate the association between TCF7L2 rs7903146 in an Iranian population. Type 2 diabetes patients (N = 258) and normal healthy control subjects (N = 168) from the same area, were examined. The ARMS-PCR (Amplification Refractory Mutation System) technique, subsequently validated by direct sequencing, was used for genotyping. Allele and genotype frequencies were significantly different between patients and controls TT vs. CT + CC [p 0.0081 OR 3.4 95%CI (1.27-11.9)] and T vs. C... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: TCF7L2; Gene; Polymorphism; T2DM. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300010 |
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| El-Hani,Charbel Niño. |
| Challenges to the gene concept have shown the difficulty of preserving the classical molecular concept, according to which a gene is a stretch of DNA encoding a functional product (polypeptide or RNA). The main difficulties are related to the overlaying of the Mendelian idea of the gene as a unit: the interpretation of genes as structural and/or functional units in the genome is challenged by evidence showing the complexity and diversity of genomic organization. This paper discusses the difficulties faced by the classical molecular concept and addresses alternatives to it. Among the alternatives, it considers distinctions between different gene concepts, such as that between the molecular and the evolutionary gene, or between gene-P (the gene as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene; Classical molecular gene concept; Mendelian gene; Genomic complexity. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300001 |
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| SIQUEIRA, F.; MENEZES, G. R. de O.; CAVIGLIONI, M. de B. C.. |
| Musculatura dupla (MD) é o termo usado para designar uma hipertrofia muscular que ocorre em várias espécies de animais, tais como bovinos (Ménissier, 1982; McPherron & Lee, 1997), ovinos (Boman et al., 2009; Miar et al., 2014), suínos (Stinckens et al., 2005, Stinckens et al., 2008), equinos (Dall?Olio et al., 2010; Hill et al., 2010a), peixes (Xu et al., 2003; Ostbye et al., 2007), roedores (McPherron et al., 1997; Szabo et al., 1998), cães (Mosher et al., 2007), aves (Sazanov et al., 1999), e, até mesmo, em humanos (Gonzalez-Cadavid et al., 1998; Schuelke et al., 2004). Esta condição é hereditária e surge no período fetal, quando ocorre aumento no número total de fi bras musculares (hiperplasia celular), e no período pós-natal, quando ocorre aumento... |
| Tipo: Livros |
Palavras-chave: Biologia Molecular; Doença Animal; Fenótipo; Gado de Corte; Gene; Genética; Hiperplasia. |
| Ano: 2022 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1148838 |
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| CORDEIRO, M. C. R.. |
| ABSTRACT: Basic concepts and methodologies concerning genetic engineering are presented in this review and is directed to people or professionales not familiarized to it. Moreover, it can be a document to clarity what is genetic engineering, the importance of its contributions to modern agriculture, medicine or livestock research and to support discussions on genetic research ethics. Covers information on structure of nucleic acids, types, functions and main processes such us DNA duplication, transcription and traduction. Important methodologies are covered about DNA/RNA extraction, electrophoresis, PCR, nucleic acid libraries production, cloning, sequencing, gene characterization in connection to main historical events of genetic engineering. It... |
| Tipo: Folhetos |
Palavras-chave: Ácido Nucléico; Biologia Molecular; Biotecnologia; Clonagem; DNA; Engenharia Genética; Gene; Marcador Genético; RNA; Organismo Transgênico. |
| Ano: 2003 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/568132 |
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| NARCISO, M. G.; BEVITORI, R.. |
| No âmbito da genômica funcional, o estudo do transcriptoma pode auxiliar na elucidação das funções dos genes. Isto é possível devido ao conhecimento existente sobre expressão gênica de que ela ocorre de maneira diferenciada nos diferentes tecidos, nas diferentes fases fenológicas, em geral, nas diferentes situações em que o organismo se encontra. Entretanto, o grande volume de dados gerados pelo NGS requer as ferramentas computacionais para serem analisados. Nesse sentido, um dos objetivos desta publicação é demonstrar a utilização de dois programas de bioinformática que são amplamente utilizados na análise de transcriptoma: Tophat e Cufflinks. |
| Tipo: Folhetos |
Palavras-chave: Sequenciamento; Gene. |
| Ano: 2014 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1003470 |
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| Registros recuperados: 61 | |
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