|
|
|
|
|
| Hagel,Jillian M.; Krizevski,Raz; Kilpatrick,Korey; Sitrit,Yaron; Marsolais,Frédéric; Lewinsohn,Efraim; Facchini,Peter J.. |
| Khat (Catha edulis Forsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenylpropylamino alkaloids (1S,2S)-cathine and (1R,2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to > 98% of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Khat; (S)-cathinone; Phenylpropylamino alkaloids biosynthesis; EST library; Gene discovery. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400017 |
| |
|
|
| CAMPOS-DE QUIROZ,HUGO. |
| Recent technological advancements have substantially expanded our ability to analyze and understand plant genomes and to reduce the gap existing between genotype and phenotype. The fast evolving field of genomics allows scientists to analyze thousand of genes in parallel, to understand the genetic architecture of plant genomes and also to isolate the genes responsible for mutations. Furthermore, whole genomes can now be sequenced. This review addresses these issues and also discusses ways to extract biological meaning from DNA data. Although genomic issuesare addressed from a plant perspective, this review provides insights into the genomic analyses of other organisms |
| Tipo: Journal article |
Palavras-chave: Genomics; Plant breeding; Gene discovery. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300013 |
| |
|
|
|
