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Registros recuperados: 59
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Detección de transgenes en variedades nativas de maíz en dos regiones del estado de Puebla. Colegio de Postgraduados
Carreón Herrera, Nayelli Itzell.
La presencia de transgenes en variedades nativas de maíz mexicano ha sido motivo de controversia. Se sospecha una posible introducción a través de las tiendas rurales DICONSA (TRD). El objetivo de este estudio fue precisar si plantas provenientes del grano expendido por las TRD al sembrarse llegan a antesis, y si esta coincide con la floración femenina de los maíces nativos de municipios de la Mixteca Baja en el Estado de Puebla. También se buscó cuantificar secuencias transgénicas, particularmente el promotor del Virus Mosaico de la coliflor (CaMV, por sus siglas en inglés) -35S en variedades nativas de maíz y en granos provenientes de TRD. Para el primer objetivo, en 2008 se establecieron dos experimentos en campo evaluando 46 poblaciones nativas y 23...
Palavras-chave: Maíz transgénico; Variedades nativas; Maíces nativos; Dinámica de floración; Promotor CaMV-35S; PCR Tiempo-Real; Flujo génico; Transgenic maize; Maize landraces; CaMV-35S promoter; RT-PCR; Gene flow; Flowering dynamics; Maestría; EDAR; Estrategias para el Desarrollo Agrícola Regional.
Ano: 2011 URL: http://hdl.handle.net/10521/439
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Detección de transgenes en variedades nativas de maíz en dos regiones del estado de Puebla. Colegio de Postgraduados
Carreón Herrera, Nayelli Itzell.
La presencia de transgenes en variedades nativas de maíz mexicano ha sido motivo de controversia. Se sospecha una posible introducción a través de las tiendas rurales DICONSA (TRD). El objetivo de este estudio fue precisar si plantas provenientes del grano expendido por las TRD al sembrarse llegan a antesis, y si esta coincide con la floración femenina de los maíces nativos de municipios de la Mixteca Baja en el Estado de Puebla. También se buscó cuantificar secuencias transgénicas, particularmente el promotor del Virus Mosaico de la coliflor (CaMV, por sus siglas en inglés) -35S en variedades nativas de maíz y en granos provenientes de TRD. Para el primer objetivo, en 2008 se establecieron dos experimentos en campo evaluando 46 poblaciones nativas y 23...
Palavras-chave: Maíz transgénico; Variedades nativas; Maíces nativos; Dinámica de floración; Promotor CaMV-35S; PCR Tiempo-Real; Flujo génico; Transgenic maize; Maize landraces; CaMV-35S promoter; RT-PCR; Gene flow; Flowering dynamics; Maestría; EDAR; Estrategias para el Desarrollo Agrícola Regional.
Ano: 2011 URL: http://hdl.handle.net/10521/439
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Evolución genética de cuatro especies de Anastrepha spp. (Díptera: Tephritidae). Colegio de Postgraduados
Teodoro Pardo, Claudia Verónica de.
El objetivo de este estudio fue analizar la variabilidad genética en diferentes especies del género Anastrepha (Anastrepha ludens, A. obliqua, A. serpentina y A. striata), estudiar el flujo genético en A. ludens entre diferentes poblaciones de México y de Guatemala mediante un análisis isoenzimático, cuantificar ADN del ganglio cerebral y glándulas salivales y explorar la hibridación genómica in situ (GISH) en cada una de las especies de moscas en condiciones de laboratorio. Se analizaron los sistemas isoenzimáticos 6PGDH, IDH, ME, MDH, GPI y GOT en las cuatro especies de moscas en condiciones de laboratorio y campo, y para el flujo genético sólo cuatro sistemas fueron estudiados. Se examinaron 30 individuos (15 hembras y 15 machos) de cada población. Los...
Palavras-chave: Anastrepha; Contenido de ADN; Citometría de flujo; Electroforesis; GISH; Isoenzimas; Flujo genético; Variabilidad genética; DNA content; Flow cytometry; Electrophoresis; Isozymes; Gene flow; Genetic variability; Doctorado; Genética.
Ano: 2011 URL: http://hdl.handle.net/10521/569
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Evolución genética de cuatro especies de Anastrepha spp. (Díptera: Tephritidae). Colegio de Postgraduados
Teodoro Pardo, Claudia Verónica de.
El objetivo de este estudio fue analizar la variabilidad genética en diferentes especies del género Anastrepha (Anastrepha ludens, A. obliqua, A. serpentina y A. striata), estudiar el flujo genético en A. ludens entre diferentes poblaciones de México y de Guatemala mediante un análisis isoenzimático, cuantificar ADN del ganglio cerebral y glándulas salivales y explorar la hibridación genómica in situ (GISH) en cada una de las especies de moscas en condiciones de laboratorio. Se analizaron los sistemas isoenzimáticos 6PGDH, IDH, ME, MDH, GPI y GOT en las cuatro especies de moscas en condiciones de laboratorio y campo, y para el flujo genético sólo cuatro sistemas fueron estudiados. Se examinaron 30 individuos (15 hembras y 15 machos) de cada población. Los...
Palavras-chave: Anastrepha; Contenido de ADN; Citometría de flujo; Electroforesis; GISH; Isoenzimas; Flujo genético; Variabilidad genética; DNA content; Flow cytometry; Electrophoresis; Isozymes; Gene flow; Genetic variability; Doctorado; Genética.
Ano: 2011 URL: http://hdl.handle.net/10521/569
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Effects of Roads and Traffic on Wildlife Populations and Landscape Function: Road Ecology is Moving toward Larger Scales Ecology and Society
van der Ree, Rodney; University of Melbourne; rvdr@unimelb.edu.au; van der Grift, Edgar A.; Alterra, Wageningen UR, Netherlands; edgar.vandergrift@wur.nl; Clevenger, Anthony P.; Western Transportation Institute, Montana State University, USA; apclevenger@gmail.com.
Tipo: Peer-Reviewed Reports Palavras-chave: Animal movement; Animal-vehicle collisions; Barrier effect; Ecological threshold; Gene flow; Habitat fragmentation; Mitigation; Population viability analysis; Road ecology; Road-effect zone; Traffic mortality; Traffic noise; Traffic volume; Transportation planning.
Ano: 2011
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Rethinking the Risk Management Process for Genetically Engineered Crop Varieties in Small-scale, Traditionally Based Agriculture Ecology and Society
Cleveland, David A; University of California, Santa Barbara; cleveland@es.ucsb.edu; Soleri, Daniela; University of California, Santa Barbara; soleri@es.ucsb.edu.
Tipo: Peer-Reviewed Reports Palavras-chave: Agricultural biotechnology; Biological diversity; Biological invasion; Crop genetic resources; Farmer participation in risk evaluation; Gene flow; Genetic engineering; Risk analysis; Risk management process; Traditionally based agricultural systems compared with industrial agriculture systems; Transgenes; Transgenic crop varieties.
Ano: 2005
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Population Genetic Aspects of Pollinator Decline Ecology and Society
Packer, Laurence; York University; bugsrus@yorku.ca; Owen, Robin; Mount Royal College and University of Calgary; rowen@mtroyal.ab.ca.
We reviewed the theory of conservation genetics, with special emphasis on the influence of haplodiploidy and other aspects of bee biology upon conservation genetic parameters. We then investigated the possibility that pollinator decline can be addressed in this way, using two meta-analytical approaches on genetic data from the Hymenoptera and the Lepidoptera. First, we compared levels of heterozygosity between the orders. As has been found previously, the haplodiploid Hymenoptera had markedly lower levels of genetic variation than the Lepidoptera. Bees had even lower levels, and bumble bees, in particular, often seemed almost monomorphic genetically. However, the statistically confounding effects of phylogeny render detailed interpretation of such data...
Tipo: Peer-Reviewed Reports Palavras-chave: Bumble bees; Effective population size; Gene flow; Haplodiploidy; Heterozygosity; Hymenoptera; Lepidoptera; Meta-analysis; Pollinator decline; Population fragmentation; Population genetics.
Ano: 2001
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Beyond Roadkill, Radiotracking, Recapture and FST—a Review of Some Genetic Methods to Improve Understanding of the Influence of Roads on Wildlife Ecology and Society
Simmons, Jody M; Monash University; Australian Centre for Biodiversity; jodymichellesimmons@yahoo.com.au; Sunnucks, Paul; Monash University; Australian Centre for Biodiversity; paul.sunnucks@sci.monash.edu.au; Taylor, Andrea C; Monash University; Australian Centre for Biodiversity; andrea.taylor@sci.monash.edu.au; van der Ree, Rodney; Australian Research Centre for Urban Ecology, Royal Botanic Gardens Melbourne; rvdr@unimelb.edu.au.
Habitat fragmentation continues to occur despite increasing evidence of its adverse effects on ecosystems. One of the major detrimental effects of roads and traffic is the creation of barriers or filters to the movement of wildlife, ultimately disconnecting some populations. Our understanding of the extent to which roads reduce the movement of biota is mostly based on field-based observational methods of inferring animal movement, and to a much smaller extent, on allele frequency-based genetic analyses. Field-based methods, as it is typically feasible to apply them, tend to be informative at fine temporal and spatial scales. Allele frequency-based genetic methods are informative at broad geographic scales but at timescales usually greater than recent...
Tipo: Peer-Reviewed Insight Palavras-chave: Barrier; Dispersal; Gene flow; Genotypic analyses; Habitat fragmentation; Road ecology.
Ano: 2010
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Insect resistance and risk assessment studies of advanced generations of basmati rice expressing two genes of Bacillus thuringiensis Electron. J. Biotechnol.
Rahman,Mahmood-ur; Rashid,Hamza; Shahid,Ahmad Ali; Bashir,Khurram; Husnain,Tayyab; Riazuddin,Sheikh.
Advanced generations of different transgenic lines of indica basmati rice (Basmati-370) expressing two unrelated Bt genes, cry1Ac and cry2A were evaluated for resistance to Yellow Stem Borer (YSB) and Rice Leaf Folder (RLF) under field conditions compared to control lines over three years (2003-2005). Homozygous lines were selected and analyzed for insect resistance, morphological, physiochemical properties and risk assessment studies. After artificial infestation of target insects, the transgenic plants showed significant resistance. Data were recorded in terms of dead hearts and white heads at vegetative and flowering stage respectively. Transgenic lines showed up to 100 and 96% resistance against yellow stem borer at vegetative and flowering stages,...
Tipo: Journal article Palavras-chave: Bio-safety; Cry1Ac; Cry2A; Gene flow; Field trials; Gene pyramiding; Insect resistance.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200008
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Genetic structure of sigmodontine rodents (Cricetidae) along an altitudinal gradient of the Atlantic Rain Forest in southern Brazil Genet. Mol. Biol.
Gonçalves,Gislene L.; Marinho,Jorge R.; Freitas,Thales R.O..
The population genetic structure of two sympatric species of sigmodontine rodents (Oligoryzomys nigripes and Euryoryzomys russatus) was examined for mitochondrial DNA (mtDNA) sequence haplotypes of the control region. Samples were taken from three localities in the Atlantic Rain Forest in southern Brazil, along an altitudinal gradient with different types of habitat. In both species there was no genetic structure throughout their distribution, although levels of genetic variability and gene flow were high.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Euryoryzomys russatus; Gene flow; Mismatch distribution; Oligoryzomys nigripes; Population expansion.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400034
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Spatial and temporal population genetic variation and structure of Nothotsuga longibracteata (Pinaceae), a relic conifer species endemic to subtropical China Genet. Mol. Biol.
Qiu,Yingjun; Liu,Yifei; Kang,Ming; Yi,Guanmei; Huang,Hongwen.
Nothotsuga longibracteata, a relic and endangered conifer species endemic to subtropical China, was studied for examining the spatial-temporal population genetic variation and structure to understand the historical biogeographical processes underlying the present geographical distribution. Ten populations were sampled over the entire natural range of the species for spatial analysis, while three key populations with large population sizes and varied age structure were selected for temporal analyses using both nuclear microsatellites (nSSR) and chloroplast microsatellites (cpSSR). A recent bottleneck was detected in the natural populations of N. longibracteata. The spatial genetic analysis showed significant population genetic differentiation across its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene flow; Glacial refugia; Nothotsuga longibracteata; Spatial genetic structure; Temporal genetic structure.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400019
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Genetic structure of Brazilian wild rice (Oryza glumaepatula Steud., Poaceae) populations analyzed using microsatellite markers Genet. Mol. Biol.
Karasawa,Marines M.G.; Vencovsky,Roland; Silva,Cynthia M.; Zucchi,Maria Imaculada; Oliveira,Giancarlo C.X.; Veasey,Elizabeth A..
Knowledge of the genetic structure and diversity of natural populations is important in developing strategies for in situ and ex situ conservation. We used eight microsatellite loci to estimate genetic structure and investigate within and between population genetic variation in eleven Brazilian wild rice (Oryza glumaepatula) populations. The study showed the following genetic diversity parameters: average number of 3.1 alleles per locus; 77.3% polymorphic loci; 0.091 observed heterozygosity and 0.393 gene diversity. F-statistics detected by microsatellite loci were: F ST = 0.491 (and R ST = 0.608), F IS = 0.780 and F IT = 0.888. No population was in Hardy-Weinberg equilibrium. The estimated apparent outcrossing rate (0.143) indicated a predominance of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Gene flow; Genetic diversity; Microsatellites; Oryza glumaepatula.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300017
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Genetic diversity and population structure of Vriesea reitzii (Bromeliaceae), a species from the Southern Brazilian Highlands Genet. Mol. Biol.
Soares,Luis Eduardo; Goetze,Márcia; Zanella,Camila M.; Bered,Fernanda.
Abstract The Southern Brazilian Highlands are composed by a mosaic of Mixed Ombrophilous Forest (MOF) and grassland formations, an interesting landscape for the study of population structure. We analyzed the genetic diversity within and among populations of the MOF-endemic bromeliad Vriesea reitzii by genotyping seven nuclear microsatellite loci in 187 individuals from six populations. We characterized levels of genetic diversity and assessed the genetic structure among populations. Vriesea reitzii populations showed high levels of genetic variation (number of alleles 28 - 43, allelic richness 3.589 - 5.531) and moderate levels of genetic differentiation (FST = 0.123, RST = 0.096). The high levels of genetic diversity may be explained by species...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mixed Ombrophilous Forest; Bromeliad; Araucaria forest; Gene flow; Conservation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200308
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Testing the Rio Doce as a riverine barrier in shaping the Atlantic rainforest population divergence in the rodent Akodon cursor Genet. Mol. Biol.
Colombi,Victor Hugo; Lopes,Silvia Ramira; Fagundes,Valéria.
Akodon cursor occurs in dense rainforest from northern (8º S) to southern (26º S) states along the Atlantic coast of Brazil. Previous karyological and molecular data revealed two major clades, one including northern (8-15º S) and the other southern (19-26º S) populations. The center of geographic distribution (15-20º S), which included the state of Espírito Santo, was identified as a potential vicariance region. Since river barriers are among the most discussed models in the study of Neotropical diversification, we examined whether the Rio Doce (19º S) plays an important role in shaping the population genetic divergence of A. cursor by including samples from Espírito Santo in the analysis. Our results showed that the northern-southern division region in...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Akodontini; Atlantic Forest; Cytochrome b; Gene flow; Phylogeography; Rodentia.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400029
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Pollen and seed flow patterns of Carapa guianensis Aublet. (Meliaceae) in two types of Amazonian forest Genet. Mol. Biol.
Martins,Karina; Raposo,Andréa; Klimas,Christie A; Veasey,Elizabeth A; Kainer,Karen; Wadt,Lúcia Helena O.
Various factors affect spatial genetic structure in plant populations, including adult density and primary and secondary seed dispersal mechanisms. We evaluated pollen and seed dispersal distances and spatial genetic structure of Carapa guianensis Aublet. (Meliaceae) in occasionally inundated and terra firme forest environments that differed in tree densities and secondary seed dispersal agents. We used parentage analysis to obtain contemporary gene flow estimates and assessed the spatial genetic structure of adults and juveniles. Despite the higher density of adults (diameter at breast height ; 25 cm) and spatial aggregation in occasionally inundated forest, the average pollen dispersal distance was similar in both types of forest (195 ± 106min terra...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene flow; Parentage analysis; Scatterhoarding; Seed dispersal; Spatial genetic structure.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500017
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Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences Genet. Mol. Biol.
Lima Júnior,Raimundo Sousa; Scarpassa,Vera Margarete.
Genetic variation was estimated in ten samples populations of Aedes aegypti from the Brazilian Amazon, by using a 380 bp fragment of the mitochocondrial NADH dehydrogenase subunit 4 (ND4) gene. A total of 123 individuals were analyzed, whereby 13 haplotypes were found. Mean genetic diversity was slightly high (h = 0.666 ± 0.029; π = 0.0115 ± 0.0010). Two AMOVA analyses indicated that most of the variation (~70%-72%) occurred within populations. The variation found among and between populations within the groups disclosed lower, but even so, highly significant values. F ST values were not significant in most of the comparisons, except for the samples from Pacaraima and Rio Branco. The isolation by distance (IBD) model was not significant (r = 0.2880; p =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Dengue vector; Genetic structure; Gene flow; Brazilian Amazon; Genetic lineages.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200032
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Allozymic variation and differentiation in the chilean blue mussel, Mytilus chilensis, along its natural distribution Genet. Mol. Biol.
Toro,Jorge E.; Castro,Grace C.; Ojeda,Johana A.; Vergara,Ana M..
Genetic differentiation in the Chilean blue mussel Mytilus chilensis (Hupé 1854) was investigated based on the variation in the allozyme frequencies of Pgm, Gpi, Icd, Me, Gsr, Lap and Pep in eight samples collected along 1800 km from Arauco (VIII Region) to Punta Arenas (XII Region). Despite the large geographic separations, values of Neis unbiased genetic distance, D (0.004-0.048) and standardised genetic variation among populations, Fst (0.011-0.055) were small. The levels of gene flow (Nm = 8) found in this study prevent the effect of differentiation among populations by genetic drift. This findings indicate that its long-lived planktotrophic larvae provides this species with considerable dispersal ability throughout its range which is favoured by the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozyme; Mytilus chilensis; Population genetics; Gene flow; Chile.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100031
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Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil Genet. Mol. Biol.
Silva,Daniela de Melo e; Cruz,Aparecido Divino da; Bastos,Rogério Pereira; Reis,Raquel Loren; Telles,Mariana Pires de Campos; Diniz-Filho,José Alexandre Felizola.
This study reports on 156 specimens of the amphibian Eupemphix nattereri, a widely distributed leiuperid, obtained from 11 municipalities of central Brazil. The extent of genetic variation was quantified by determining the mean number of alleles per locus and the proportion of polymorphic loci. An analysis of molecular variance (AMOVA) was performed on the random amplified polymorphic DNA (RAPD) haplotypes. The genetic distances obtained by calculating pairwise phist among local samples were used to determine population relationships using the unweighted pair-group method (UPGMA) and non-metric multidimensional scaling (NMDS). The cophenetic correlation was calculated to confirm agreement between the genetic matrix and the unweighted pair group method with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eupemphix nattereri; Gene flow; Population structure; RAPD markers.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600022
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High genetic diversity and connectivity in Colossoma macropomum in the Amazon basin revealed by microsatellite markers Genet. Mol. Biol.
Fazzi-Gomes,Paola; Guerreiro,Sávio; Palheta,Glauber David Almeida; Melo,Nuno Filipe Alves Correa de; Santos,Sidney; Hamoy,Igor.
Abstract Colossoma macropomum is the second largest scaled fish of the Amazon. It is economically important for commercial fisheries and for aquaculture, but few studies have examined the diversity and genetic structure of natural populations of this species. The aim of this study was to investigate the levels of genetic variability and connectivity that exist between three natural populations of C. macropomum from the Amazon basin. In total, 247 samples were collected from the municipalities of Tefé, Manaus, and Santarém. The populations were genotyped using a panel of 12 multiplex microsatellite markers. The genetic diversity found in these populations was high and similar to other populations described in the literature. These populations showed a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tambaqui; Genetic variability; Gene flow; Genetic structure; Single sequence repeats.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100142
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Estimating dispersal and gene flow in the neotropical freshwater turtle Hydromedusa maximiliani (Chelidae) by combining ecological and genetic methods Genet. Mol. Biol.
Souza,Franco L.; Cunha,Anderson F.; Oliveira,Marcos A.; Pereira,Gonçalo A.G.; Reis,Sérgio F. dos.
Hydromedusa maximiliani is a vulnerable neotropical freshwater turtle endemic to mountainous regions of the Atlantic rainforest in southeastern Brazil. Random amplified polymorphic DNA (RAPD) was used to estimate the gene flow and dispersal for individuals inhabiting rivers and streams within a drainage. Nine primers generated 27 scoreable bands, of which 9 (33%) were polymorphic and produced 12 RAPD phenotypes. The gene flow estimates (Nm) among turtles inhabiting different rivers and streams were variable, ranging from 0.09 to 3.00 (mean: 0.60). For some loci, the rates of gene flow could offset population differentiation (Nm > 1), whereas for others random genetic drift could result in population divergence (Nm < 1). Since the genetic variation of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydromedusa maximiliani; Dispersal; Gene flow; Conservation.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200007
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