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Registros recuperados: 45
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Historia de vida y genética cuantitativa de cuatro poblaciones del cocodrilo de pantano (Crocodylus moreletii) bajo condiciones de cautiverio en el trópico mexicano. Colegio de Postgraduados
Serna Lagunes, Ricardo.
Se evaluó la variación morfológica, crecimiento corporal, estructura, variación genética y tamaño efectivo de población (Ne) de cuatro poblaciones de Crocodylus moreletii, manejadas en la UMA Cacahuatal, situada en Veracruz, México. La variación morfológica y el crecimiento corporal se evaluaron aplicando un análisis multivariado de covarianza (MANCOVA) y un análisis de varianza (ANOVA), respectivamente. La descripción de la estructura genética se realizó acorde a los principios de Hardy-Weinberg y se evaluó la variación genética con un análisis de varianza molecular (AMOVA), que se corroboró con los estadísticos F. El Ne se determinó a partir del total de cocodrilos reproductivos y se obtuvo la tasa de incremento de endogamia. La población de Villa Juárez...
Palavras-chave: Morfología; Crecimiento corporal; Estructura genética poblacional; Variación genética; Tamaño efectivo de población; Morphology; Body growth; Genetic structure; Genetic variation; Effective population size; Maestría; Agroecosistemas Tropicales.
Ano: 2010 URL: http://hdl.handle.net/10521/191
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Estructura genética de poblaciones de Oligonychus perseae y Oligonychus punicae (Acari: Tetranychidae) en huertos de aguacate. Colegio de Postgraduados
Guzmán Valencia, Stephanie.
La identidad y estructura genética de poblaciones de Oligonychus punicae y O. perseae (Acari: Tetranychidae) fue estudiada en siete localidades productoras de aguacate Persea americana var. Hass. La identidad de los ácaros fue determinada morfológica y molecularmente mediante la secuenciación de una fracción del gen Citocromo Oxidasa subunidad I (COI). La variación genética intraespecífica de diferentes poblaciones de ambas especies de ácaros fue estudiada mediante la secuenciación de las regiones ITS1 e ITS2 del rADN. El análisis filogenético de las secuencias parciales del gen COI separó adecuadamente las dos especies de ácaros, confirmando los resultados de la identificación morfológica. El análisis de las secuencias de las regiones ITS1 e ITS2 del...
Palavras-chave: COI; ITS1; ITS2; Estructura genética; Entomología y Acarología; Maestría; Genetic structure.
Ano: 2012 URL: http://hdl.handle.net/10521/1765
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High genetic diversity and small genetic variation among populations of Magnolia wufengensis (Magnoliaceae), revealed by ISSR and SRAP marker Electron. J. Biotechnol.
Chen,Liyuan; Chen,Faju; He,Suichao; Ma,Luyi.
Background Genetic diversity and genetic variation of 10 populations and subpopulations of Magnolia wufengensis, a new and endangered endemic species, were examined by inter simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) molecular markers. Compared with other endangered endemic Magnolia taxa, M. wufengensis holds a relatively high level of genetic variation. Result Total genetic diversity was found to be 87.7% for ISSR and 88.0% for SRAP markers. For polymorphic loci (P), the effective mean number of alleles (Ae) was 1.414 for ISSR markers and 1.458 for SRAP markers, while the mean expected heterozygosity (H) was 0.256 using ISSR and 0.291 for SRAP markers. Within-population variation was estimated for P as 74.9% using...
Tipo: Journal article Palavras-chave: Genetic structure; ISSR; Population genetic diversity; SRAP.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600003
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The genetic diversity of Sardinian myrtle (Myrtus communis L.) populations Electron. J. Biotechnol.
Melito,Sara; Chessa,Innocenza; Erre,Patrizia; Podani,János; Mulas,Maurizio.
Background: The myrtle (Myrtus communis) is a common shrub widespread in the Mediterranean Basin. Its fruit and leaves exhibit antioxidant, antibacterial and antifungal properties, and are used for their content of essential oils and for their medicinal properties, but most commonly as an ingredient in locally made liquor. The uncontrolled exploitation of natural stands has reduced both the species' geographical coverage and the size of individual populations. The selection of genotypes for controlled cultivation requires a characterization of the genetic diversity present both within and between populations. Results: Genotypic variation was evaluated using ISSR profiling and genetic diversity characterized using standard population genetics approaches....
Tipo: Journal article Palavras-chave: Domestication; Genetic structure; Germplasm; ISSR genotyping.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600007
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Genetic diversity in two Italian almond collections Electron. J. Biotechnol.
Rigoldi,Maria Pia; Rapposelli,Emma; De Giorgio,Donato; Resta,Paolo; Porceddu,Andrea.
Background Sweet-seeded domesticated almonds were brought to the Mediterranean Basin from central Asia about 4000 years ago. In Italy, most of the almonds produced are cultivated in the southern part of the country. Local populations of the tree in Sardinia are largely seed-derived and mostly self-incompatible, so have developed extensive genetic diversity. The need to protect biodiversity has prompted a revived interest in local genetic materials in almond. Two Italian collections have been established, one in Sardinia and the other in Apulia. These collections were the focus of the present evaluation of genetic diversity. Results Eleven SSRs (microsatellites) were used for fingerprinting. The Sardinian germplasm was highly polymorphic, revealing a mean...
Tipo: Journal article Palavras-chave: Biodiversity; Genetic structure; Prunus amygdalus; SSR.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100008
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RAPD markers indicate the occurrence of structured populations in a migratory freshwater fish species Genet. Mol. Biol.
Hatanaka,Terumi; Galetti Jr.,Pedro Manoel.
Many factors have contributed to the destruction of fish habitats. Hydroelectric dams, water pollution and other environmental changes have resulted in the eradication of natural stocks. The aim of this study was to detect the genetic variation in Prochilodus marggravii from three collection sites in the area of influence of the Três Marias dam (MG) on the São Francisco river (Brazil), using the RAPD technique. The results obtained revealed that the fish in the downstream region nearest the dam have a higher similarity coefficient than those from the other sampling sites that may be related to differences in environmental characteristics in these regions. Additionaly, significant differences in the band frequencies were observed from one collection site to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prochilodus; RAPD; Genetic structure; Conservation genetic; Fish.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100004
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Characterization of 13 microsatellite loci developed from Meconopsis horridula Genet. Mol. Biol.
Zhao,Yu; Zhang,Shi-Bao; Yang,Jing; Zhang,Lu.
Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic structure; Heterozygosity; Meconopsis horridula; Microsatellite markers; Polymorphism.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300026
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Association mapping for yield and grain quality traits in rice (Oryza sativa L.) Genet. Mol. Biol.
Borba,Tereza Cristina de Oliveira; Brondani,Rosana Pereira Vianello; Breseghello,Flávio; Coelho,Alexandre Siqueira Guedes; Mendonça,João Antônio; Rangel,Paulo Hideo Nakano; Brondani,Claudio.
Association analysis was applied to a panel of accessions of Embrapa Rice Core Collection (ERiCC) with 86 SSR and field data from two experiments. A clear subdivision between lowland and upland accessions was apparent, thereby indicating the presence of population structure. Thirty-two accessions with admixed ancestry were identified through structure analysis, these being discarded from association analysis, thus leaving 210 accessions subdivided into two panels. The association of yield and grain-quality traits with SSR was undertaken with a mixed linear model, with markers and subpopulation as fixed factors, and kinship matrix as a random factor. Eight markers from the two appraised panels showed significant association with four different traits,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Association analysis; Core collection; Genetic structure.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300023
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High levels of genetic differentiation and selfing in the Brazilian cerrado fruit tree Dipteryx alata Vog. (Fabaceae) Genet. Mol. Biol.
Tarazi,Roberto; Moreno,Maria Andréia; Gandara,Flávio Bertin; Ferraz,Elza Martins; Moraes,Mário Luiz Teixeira; Vinson,Christina Cleo; Ciampi,Ana Yamaguishi; Vencovsky,Roland; Kageyama,Paulo Yoshio.
Dipteryx alata is a native fruit tree species of the cerrado (Brazilian savanna) that has great economic potential because of its multiple uses. Knowledge of how the genetic variability of this species is organized within and among populations would be useful for genetic conservation and breeding programs. We used nine simple sequence repeat (SSR) primers developed for Dipteryx odorata to evaluate the genetic structure of three populations of D. alata located in central Brazil based on a leaf sample analysis from 101 adults. The outcrossing rate was evaluated using 300 open-pollinated offspring from 25 seed-trees. Pollen dispersal was measured by parentage analysis. We used spatial genetic structure (SGS) to test the minimal distance for harvesting seeds...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dipteryx alata; Genetic structure; Mating system; SSR.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100015
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Analysis by RAPD of the genetic structure of Astyanax altiparanae (Pisces, Characiformes) in reservoirs on the Paranapanema River, Brazil Genet. Mol. Biol.
Leuzzi,Maria Sueli Papa; Almeida,Fernanda Simões de; Orsi,Mário Luís; Sodré,Leda Maria Koelblinger.
In this study, the RAPD technique was used to analyze the genetic structure of populations of the fish Astyanax altiparanae (Characidae, Tetragonopterinae) living in the lower, middle and upper Paranapanema River, Brazil. The aim was to assess this structure regarding fish handling and conservation programs. The genetic variability (P) was found to be 42.64%, 75% and 75% in the low, middle and upper reaches, respectively. The dendrogram of genetic similarity, obtained by comparative analysis of the sets of samples from the three sites, showed the formation of three clusters. All of the genetic parameters used indicate that the population in the lower Paranapanema is genetically different from those in the middle and upper sections. The theta P test shows...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astyanax altiparanae; RAPD; Genetic structure; Genetic conservation; Fish.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300009
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Genetic diversity in populations of African mahogany (Khaya grandioliola C. DC.) introduced in Brazil Genet. Mol. Biol.
Soares,Sabrina Delgado; Bandeira,Ludmila Ferreira; Ribeiro,Stela Barros; Telles,Mariana Pires de Campos; Silva,João Augusto da; Borges,Canrobert Tormin; Coelho,Alexandre Siqueira Guedes; Novaes,Evandro.
Abstract Given its high-valued wood, the African mahogany (Khaya grandifoliola) has been envisaged as a renewable source of tropical hardwoods in Brazil. However, there are concerns about the hypothesized low diversity among the few K. grandifoliola germplasm sources introduced in the country. Using eight microsatellite markers, we evaluated the genetic diversity and divergence among 53 superior trees selected from three provenances of K. grandifoliola located in the state of Para. These populations are among the oldest plantations (>15 years) in Brazil and, therefore, the country's main seed sources. The average number of alleles per locus was 5.9, expected heterozygosity was moderate (^=0.56) and lower than the high observed heterozygosity (HO=0.74)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; SSR; Genetic structure; Genetic divergence; Hardwood.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400301
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Genetic structure of red-handed howler monkey populations in the fragmented landscape of Eastern Brazilian Amazonia Genet. Mol. Biol.
Bastos,Heitor B.; Gonçalves,Evonnildo C.; Ferrari,Stephen F.; Silva,Artur; Schneider,Maria Paula C..
We genotyped 15 microsatellite loci in order to evaluate the effects of habitat fragmentation, caused by flooding of the Tucuruí reservoir, on the genetic structure of Alouatta belzebul in eastern Amazonia. The analysis included two populations sampled in 1984, representing both margins of the Tocantins river, and three populations sampled 18 years later. Minimal differences in the diversity levels between present-day (Ho = 0.62-0.69 and A R = 6.07-7.21) and pre-flooding (Ho = 0.60-0.62 and A R = 6.27-6.77) populations indicated there was no significant loss of genetic variability, possibly because of successful management strategies applied during the flooding. The changes observed were limited to shifts in the composition of alleles, which presumably...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alouatta belzebul; Amazonia; Conservation; Genetic structure; Habitat fragmentation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400027
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Genetic structure of Argentinean hexaploid wheat germplasm Genet. Mol. Biol.
Vanzetti,Leonardo S.; Yerkovich,Nadia; Chialvo,Eugenia; Lombardo,Lucio; Vaschetto,Luis; Helguera,Marcelo.
The identification of genetically homogeneous groups of individuals is an ancient issue in population genetics and in the case of crops like wheat, it can be valuable information for breeding programs, genetic mapping and germplasm resources. In this work we determined the genetic structure of a set of 102 Argentinean bread wheat (Triticum aestivum L.) elite cultivars using 38 biochemical and molecular markers (functional, closely linked to genes and neutral ones) distributed throughout 18 wheat chromosomes. Genetic relationships among these lines were examined using model-based clustering methods. In the analysis three subpopulations were identified which correspond largely to the origin of the germplasm used by the main breeding programs in Argentina.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wheat; Genetic structure; Molecular markers; Genetic diversity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300014
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Genetic diversity in cultivated carioca common beans based on molecular marker analysis Genet. Mol. Biol.
Perseguini,Juliana Morini Küpper Cardoso; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Colombo,Carlos Augusto; Carbonell,Sérgio Augusto Moraes; Mondego,Jorge Mauricio Costa; Gazaffi,Rodrigo; Garcia,Antonio Augusto Franco; Campos,Tatiana de; Souza,Anete Pereira de; Rubiano,Luciana Benchimol.
A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFLPs; Genetic structure; Genetic variability; Phaseolus vulgaris L.; SSRs.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017
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Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences Genet. Mol. Biol.
Lima Júnior,Raimundo Sousa; Scarpassa,Vera Margarete.
Genetic variation was estimated in ten samples populations of Aedes aegypti from the Brazilian Amazon, by using a 380 bp fragment of the mitochocondrial NADH dehydrogenase subunit 4 (ND4) gene. A total of 123 individuals were analyzed, whereby 13 haplotypes were found. Mean genetic diversity was slightly high (h = 0.666 ± 0.029; π = 0.0115 ± 0.0010). Two AMOVA analyses indicated that most of the variation (~70%-72%) occurred within populations. The variation found among and between populations within the groups disclosed lower, but even so, highly significant values. F ST values were not significant in most of the comparisons, except for the samples from Pacaraima and Rio Branco. The isolation by distance (IBD) model was not significant (r = 0.2880; p =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Dengue vector; Genetic structure; Gene flow; Brazilian Amazon; Genetic lineages.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200032
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Population genetic analysis of Caiman crocodilus (Linnaeus, 1758) from South America Genet. Mol. Biol.
Vasconcelos,William R.; Hrbek,Tomas; Silveira,Ronis da; Thoisy,Benoit de; Marioni,Boris; Farias,Izeni P..
The genetic structure of Caiman crocodilus was investigated using a 1085 bp mtDNA fragment of the cytochrome b gene. Inferences were based on 125 individuals from nine localities in Peru, Brazil and French Guiana. With the exception of Mamirauá Lake, Anavilhanas Archipelago and the Tapará Community which show a signal of demographic expansion, the sampled localities are in a mutation-drift genetic equilibrium. Divergence between the Amazon basin and extra-Amazon basin localities is significant; however, inference from Nested Clade Analysis cannot distinguish between continuous range expansion, long distance colonization or past fragmentation; however, past fragmentation is unlikely due to low number of mutational steps separating these two regions. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic structure; Phylogeography; Genetic diversity; Demographic expansion; Cytochrome b; Caiman crocodilus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200006
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Developing a common bean core collection suitable for association mapping studies Genet. Mol. Biol.
Perseguini,Juliana Morini Küpper Cardoso; Silva,Gliciane Micaele Borges; Rosa,João Ricardo Bachega Feijó; Gazaffi,Rodrigo; Marçal,Jéssica Fernanda; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando; Zucchi,Maria Imaculada; Garcia,Antonio Augusto Franco; Benchimol-Reis,Luciana Lasry.
Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular markers; Genetic diversity; Genetic structure; Microsatellites; Phaseolus vulgaris L.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100067
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High genetic diversity and connectivity in Colossoma macropomum in the Amazon basin revealed by microsatellite markers Genet. Mol. Biol.
Fazzi-Gomes,Paola; Guerreiro,Sávio; Palheta,Glauber David Almeida; Melo,Nuno Filipe Alves Correa de; Santos,Sidney; Hamoy,Igor.
Abstract Colossoma macropomum is the second largest scaled fish of the Amazon. It is economically important for commercial fisheries and for aquaculture, but few studies have examined the diversity and genetic structure of natural populations of this species. The aim of this study was to investigate the levels of genetic variability and connectivity that exist between three natural populations of C. macropomum from the Amazon basin. In total, 247 samples were collected from the municipalities of Tefé, Manaus, and Santarém. The populations were genotyped using a panel of 12 multiplex microsatellite markers. The genetic diversity found in these populations was high and similar to other populations described in the literature. These populations showed a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tambaqui; Genetic variability; Gene flow; Genetic structure; Single sequence repeats.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100142
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Molecular approaches reveal weak sibship aggregation and a high dispersal propensity in a non‐native fish parasite ArchiMer
Prunier, Jérôme G.; Saint‐pé, Keoni; Blanchet, Simon; Loot, Géraldine; Rey, Olivier.
Inferring parameters related to the aggregation pattern of parasites and to their dispersal propensity are important for predicting their ecological consequences and evolutionary potential. Nonetheless, it is notoriously difficult to infer these parameters from wildlife parasites given the difficulty in tracking these organisms. Molecular‐based inferences constitute a promising approach that has yet rarely been applied in the wild. Here, we combined several population genetic analyses including sibship reconstruction to document the genetic structure, patterns of sibship aggregation, and the dispersal dynamics of a non‐native parasite of fish, the freshwater copepod ectoparasite Tracheliastes polycolpus. We collected parasites according to a hierarchical...
Tipo: Text Palavras-chave: Full‐sibs; Genetic structure; Parasite dispersal; Parentage analysis; Tracheliastes polycolpus; Transmission.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00693/80499/83694.pdf
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Beyond skepticism: uncovering cryptic refugia using multiple lines of evidence ArchiMer
De Lafontaine, Guillaume; Amasifuen Guerra, Carlos Alberto; Ducousso, Alexis; Sanchez-goni, Maria-fernanda; Petit, Remy J..
The increasingly popular opinion that temperate plant and animal species persisted in many small refugia in Europe north of the main southern peninsular refugia directly challenges a classical paradigm. As indirect evidence for the existence of such glacial microrefugia readily accumulates, it is interesting to note that the few direct in situ occurrences of fossils testifying their local existence are almost always debated. Our recent study on European beech (Fagus sylvatica) refugia in south-western France was no exception to this trend. Yet, as mentioned by the philosopher Bertrand Russell, it is not possible ‘to get anywhere if we start from skepticism. We must start from a broad acceptance of whatever seems to be knowledge and is not rejected for some...
Tipo: Text Palavras-chave: Cryptic glacial refugia; Fagus sylvatica (European beech); Genetic structure; Heinrich stadials 1 and 2; Last glacial maximum; Late Pleniglacial interval; Microrefugia; Soil macrofossil charcoal.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00289/40066/38821.pdf
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