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Transferability of heterologous primers in Brycon falcatus Acta Amazonica
PENHA,Diego dos Santos; SOUZA,Felipe Pinheiro de; LIMA,Ed Christian Suzuki de; URREA-ROJAS,Angela Maria; PANDOLFI,Victor César Freitas; YAMACHITA,Andrei Lincoln; POVH,Jayme Aparecido; LEITE,Natalia Gonçalves; PEREIRA,Ulisses de Pádua; LOPERA-BARRERO,Nelson Mauricio.
ABSTRACT The genusBryconcomprises fish species of significant socioeconomic and biological importance in Brazil. Despite that, the genetic knowledge about these species is scarce, especially regardingBrycon falcatus. Thus, the objective of this study was to evaluate the transferability of heterologous microsatellite primers inB. falcatus for the first time. Heterologous primers obtained from B. opalinus, B. hilarii, B. insignis, B. orbignyanus, B. amazonicus, Prochilodus argenteus, Prochilodus lineatus, Piaractus mesopotamicus, and Colossoma macropomum were evaluated. The primers that showed the best amplification patterns were applied to a sample of 22 individuals and the genetic parameters were calculated. Nine primers displayed satisfactory...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bryconidae; Cross amplification; Genetic conservation; Genetic variability; SSR.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000300232
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A NEW WORLD MONKEY MICROSATELLITE (AP74) HIGHLY CONSERVED IN PRIMATES Acta biol.Colomb.
OKLANDER,LUCIANA INÉS; STEINBERG3,ELIANA RUTH; DOLORES MUDRY,MARTA.
Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Primates; Microsatellite markers; PCR amplification; Non-invasive sampling; Genomic conservation; Genetic variability.
Ano: 2012 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000100007
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Morphophysiological characterization in artichoke accessions aimed at selecting materials for in natura consumption - doi: 10.4025/actasciagron.v34i4.14551 Agronomy
Costa, Angélica Reolon da; Universidade de Passo Fundo; Grando, Magali Ferrari; Universidade de Passo Fundo.; Scheffer-Basso, Simone Meredith; Universidade de Passo Fundo; Cravero, Vanina Pamela; Universidade Nacional de Rosário.
A morphophysiological characterization of 19 accessions within an artichoke germplasm collection was conducted with the aim of determining genetic variability and to select materials suitable for in natura consumption to be used in genetic breeding. The collection was composed of commercial accessions, materials collected in South Brazil, and of foreign origin. Twenty quantitative, five multicategorical and two binary traits were evaluated. Quantitative data were submitted to a multivariate analysis. Genetic variability was observed for almost all characteristics. The traits with the greatest relative contribution to genetic divergence, which accounted for 79.54% of the variability, were as follows: the thickness and diameter bottom, bottom fresh mass/head...
Tipo: Pesquisa Empírica de Campo Palavras-chave: 5.01.00.00-9 Agronomia; 5.01.03.05-9 Melhoramento Vegetal Cynara cardunculus var. scolymus (L.) Fiori; Genetic variability; Germplasm; Multivariate analysis Melhoramento Vegetal.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/14551
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Higher-precision experimental statistics for the selection of early and upright common bean lines Agronomy
Ribeiro, Nerinéia Dalfollo; Steckling, Skarlet De Marco; Maziero, Sandra Maria; Klasener, Greice Rosana.
The development of early and upright common bean cultivars is necessary to meet the demand of producers. The objectives of this work were to evaluate more precise experimental statistics for the selection of early and upright common bean lines and identify the traits that provide better genetic differentiation between lines. For this purpose, 156 common bean lines of different grain colours were evaluated in 23 experiments carried out in the southern region of Brazil between 1998 and 2015. The traits related to earliness (flowering and cycle) and upright plant architecture (lodging, insertion of the first pod and insertion of the last pod) were evaluated with high experimental precision by determining F-test values for genotype (Fc), heritability (h2), and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Genetic variability; Statistical parameters; Selection efficiency..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42725
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Marcadores moleculares RAPD e descritores morfológicos na avaliação da diversidade genética de goiabeiras (Psidium guajava L.) - doi: 10.4025/actasciagron.v32i4.4720 Agronomy
Gomes Filho, Aroldo; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF; Oliveira, Jurandi Gonçalves de; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF; Viana, Alexandre Pio; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF; Siqueira, Ana Paula de Oliveira; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF; Oliveira, Marcos Góes; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF; Pereira, Messias Gonzaga; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF.
O conhecimento da variabilidade genética e fenotípica entre diferentes acessos de goiabeiras é importante para se apoiar programas de melhoramento dessa espécie na região Norte Fluminense que carece de novas culturas capazes de gerar renda aos produtores locais. O objetivo deste trabalho foi avaliar a divergência genética entre seis cultivares e 19 acessos de goiabeiras, por meio de marcadores moleculares RAPD e características morfoagronômicas. Foram obtidas 117 marcas polimórficas, utilizando-se 28 iniciadores. Os resultados mostraram uma concordância parcial entre os métodos de agrupamentos estudados, com a formação de 12 grupos. O acesso Vita 3 e o acesso 6 foram os mais divergentes, apresentando distância genética de 0,663. A análise comparativa dos...
Tipo: Pesquisa de campo e laboratório Palavras-chave: 5.01.03.05-9 Polimorfismo; Variabilidade genética; Método de otimização de Tocher; Método hierárquico UPGMA Melhoramento Vegetal Polymorphic; Genetic variability; Tocher’s optimization method; UPGMA cluster analysis.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/4720
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Cycle, canopy architecture and yield of common bean genotypes (Phaseolus vulgaris) in Santa Catarina State - doi: 10.4025/actasciagron.v35i1.15516 Agronomy
Zilio, Marcio; Universidade do Estado de Santa Catarina; Souza, Clovis Arruda; Universidade do Estado de Santa Catarina; Coelho, Cileide Maria Medeiros; Universidade do Estado de Santa Catarina; Miquelluti, David Jose; Universidade do Estado de Santa Catarina; Michels, Adelina Ferreira; Universidade do Estado de Santa Catarina.
The objective of this study was to identify the phenotypic and genotypic correlations between the plant cycle and plant habit and the effect on yield in landrace bean genotypes. The experiment was conducted in Joaçaba and Lages, Santa Catarina State for the 2008/2009 crop using 26 bean genotypes: 22 landrace and 4 commercial genotypes obtained from the UDESC. We evaluated the number of days from emergence to flowering, number of days from flowering to physiological maturity and number of days from emergence to physiological maturity in relation to the genotypes’ cycle. The aerial plant architecture characteristics evaluated were the growth habit, plant habit, plant height, stem diameter, height of the first pod insertion and number of nodes on the main...
Tipo: Pesquisa científica de campo e laboratório Palavras-chave: 5.01.03.00-8 Fitotecnia 5.01.03.01-6 Manejo e Tratos Culturais trail analysis; Genetic variability; Short cycle; Upright habit 5.01.03.00-8 Fitotecnia 5.01.03.01-6 Manejo e Tratos Culturais.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15516
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Characterization of race 65 of Colletotrichum lindemuthianum by sequencing ITS regions Agronomy
Coelho, Marcela; Gonçalves-Vidigal, Maria Celeste; Sousa, Lorenna Lopes de; Nunes, Maria Paula Barion Alves; Azevedo, Rafhael Felipin; Galván, Marta Zulema.
The present work aimed characterize isolates of C. lindemuthianum race 65 from different regions in Brazil by ITS sequencing. A total of 17 isolates of race 65, collected in the states of Mato Grosso, Minas Gerais, Paraná, Santa Catarina and São Paulo, were studied. Analysis of the sequences of isolates 8, 9, 12, 14 and 15 revealed the presence of two single nucleotide polymorphisms (SNPs) in the ITS1 region at the same positions. These isolates, when analyzed together with the sequence of isolate 17, revealed a SNP in the ITS2 region. The highest genetic dissimilarity, observed between isolates 11 and  3 and between isolates 11 and 10, was 0.772. In turn, isolates 7 and 2 were the most similar, with a value of 0.002 for genetic distance. The phylogenetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.00.00-9; 5.01.03.00-8 anthracnose; Genetic variability; Pathogens; Dissimilarity Melhoramento Vegetal.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/30586
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Agrupamento de acessos e cultivares de três espécies de Brachiaria por RAPD - DOI: 10.4025/actasciagron.v30i4.5298 Agronomy
Ambiel, Ana Claudia; UNOESTE; Guaberto, Luciana Machado; UNOESTE; Vanderlei, Talita Marques; UNOESTE; Machado Neto, Nelson Barbosa; UNOESTE.
O propósito deste trabalho foi determinar a similaridade genética entre acessos de germoplasma e cultivares comerciais de três espécies de Brachiaria (inter e intraespecífica), por meio de marcadores RAPD. Sementes foram utilizadas como fonte de DNA. 10 “primers” decâmeros foram selecionados de 120 “primers” avaliados, produzindo 107 bandas polimórficas, as quais foram utilizadas para a análise de variância molecular (Amova), o coeficiente de similaridade de Jaccard e índices de fixação gênica. 24,40% da variabilidade genética total estão contidas entre as espécies e 75,60% dentro destas, com índice de fixação gênica (FST) 0,24. No dendrograma, houve a formação de dois ramos, um formado por P. maximum e B. arrecta e o outro subdividido em três: todas...
Palavras-chave: 5.02.01.03-4 Genética e Melhoramento Florestal Brachiaria; Marcadores moleculares; Variabilidade genética; Dendrograma; Índice de fixação gênica. Brachiaria; Molecular markers; Genetic variability; Dendrogram; Genetic fixation index.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5298
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Use of microsatellite markers to assess the identity and genetic diversity of Vitis labrusca and Vitis rotundifolia cultivars Agronomy
Schuck, Mariane Ruzza; Universidade Federal do Paraná; Biasi, Luiz Antonio; Universidade Federal do Paraná; Moreira, Flavia Maia; Instituto Federal de Educação, Ciência e Tecnologia de Santa Catarina; Silva, Aparecido Lima da; Universidade Federal de Santa Catarina; Riaz, Summaira; University of California; Walker, Michael Andrew; University of California.
Ten grapevine cultivars were genotyped at eight microsatellite loci to characterize their identity and genetic diversity. Of these, nine cultivar profiles matched with those of databases and ‘Magoon’ matched with ‘Regale’ in the present study and ‘Regale’ in the University of California (Davis) database, implicating a likely error in planting. The number of alleles ranged from 5 (VVM5) to 9 (VVMD31), and the observed heterozygosity ranged from 37.14 (VVMD5) to 97.14% (VVMD27), with no significant differences in relation to the expected values for any of the loci, with the exception of VVMD5. The polymorphism information content values were observed to be above 0.25 in more than 85% of the loci analyzed, and VVMD31 was the most informative. The UPGMA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agronomia; Melhoramento Vegetal; Fitotecnia grapevine; Genetic variability; Molecular marker Melhoramento Vegetal.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/17869
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A comparison of RAPD and ISSR markers reveals genetic diversity among sweet potato landraces (Ipomoea batatas (L.) Lam.) - doi: 10.4025/actasciagron.v34i2.12616 Agronomy
Moulin, Monique Moreira; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Rodrigues, Rosana; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Gonçalves, Leandro Simões Azeredo; Universidade Estadual do Norte Fluminense; Sudré, Cláudia Pombo; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Pereira, Messias Gonzaga; Universidade Estadual do Norte Fluminense Darcy Ribeiro.
Small farmers have played an important role in maintaining genetic resources of traditional varieties. Collecting and characterizing germplasm, especially landraces and heirloom varieties, are strategic activities that would create a knowledge base describing the traditional varieties of a given region and reduce the risk of genetic erosion. The objectives of this work were as follows: collect sweet potato roots from rural properties and local markets in Rio de Janeiro State, Brazil; characterize the accessions using RAPD and ISSR molecular markers, comparing the efficiency in discrimination of these two types of molecular markers; and estimate the genetic variability of the population. Fifty-nine samples from rural properties and 19 from local markets...
Palavras-chave: Genetic resources; Germplasm characterization; Genetic variability; Heirloom seeds.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12616
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Genetic diversity among Brazilian okra landraces detected by morphoagronomic and molecular descriptors Agronomy
Massucato, Luana Rainieri; Nakamura, Karina Kazue; Ruas, Paulo Mauricio; Zefa, Douglas Mariani; Silva, Derly José Henrique da; Gonçalves, Leandro Simões Azeredo.
The conservation of okra landraces [Abelmoschus esculentus (L.) Moench] in gene banks is essential for the success of their use in breeding programmes. This study evaluated the genetic diversity among okra landraces in Brazil based on morphoagronomic descriptors and AFLP markers. We studied 30 accessions of the vegetable gene bank of the Universidade Federal de Viçosa. To this end, 17 morphoagronomic descriptors and five combinations of AFLP primers were used. Genetic parameters were estimated for the quantitative traits and the accessions were grouped by Ward’s method, using the Gower’s and Jaccard’s distance measures, respectively, for the morphoagronomic and molecular data. Polymorphisms were observed for all qualitative traits, while the quantitative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Molecular markers; Phenotype; Bayesian analysis.; Recursos Genéticos.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/43426
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Avaliação da variabilidade das gerações G0 e F1 da linhagem GIFT de tilápia do Nilo (Oreochromis niloticus) por RAPD - DOI: 10.4025/actascianimsci.v30i2.4708 Animal Sciences
Lupchinski Junior, Enio; UEM; Vargas, Lauro; UEM; Povh, Jayme Aparecido; UEM; Ribeiro, Ricardo Pereira; UEM; Mangolin, Claudete Aparecida; UEM; Barrero, Nelson Maurício Lopera; UEM.
Este trabalho teve como objetivo analisar, pela técnica RAPD, a variabilidade e a divergência genética de duas gerações da linhagem GIFT. Foram estimados parâmetros para os reprodutores (G0) e para a progênie (F1). A variabilidade genética foi determinada pela porcentagem de loci polimórficos e pelo índice de Shannon. As gerações apresentaram 69,6% de loci polimórficos (G0) e 60,0% de polimorfismo (F1). Os valores para o índice de Shannon foram de 0,367 para a geração G0 e de 0,317 para a F1. Os valores de divergência genética, calculados pelo teste de Mantel, foram de 0,213 para a G0 e 0,208 para a geração F1. Os resultados obtidos indicaram que houve perda da variabilidade genética da geração G0 para a F1. No entanto, um fato a ser destacado foi a alta...
Palavras-chave: 5.05.04.00-2 Reprodução Animal índice de Shannon; Gerações de cultivo; Divergência genética; Variabilidade genética. Shannon index; Cultivated generations; Genetic divergence; Genetic variability.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4708
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Diversidade genética de três estoques de piapara (Leporinus elongatus), utilizando RAPD - DOI: 10.4025/actascianimsci.v30i2.4710 Animal Sciences
Gomes, Patricia Cristina; UEM; Ribeiro, Ricardo Pereira; UEM; Barrero, Nelson Lopera; UEM; Povh, Jayme Aparecido; UEM; Vargas, Lauro; UEM; Sirol, Rodolfo Nardez; Duke Energy International Geração Paranapanema.
Recentemente a produção aquícola brasileira tem apresentado grande progresso. Dentre as espécies nativas cultivadas no Brasil, a piapara (Leporinus elongatus) tem sido amplamente preconizada. Com objetivo de avaliar os programas de repovoamento, foram analisadas a variabilidade e a divergência genética de três estoques de piapara com a técnica de RAPD (Random Amplified Polymorphic). O primeiro estoque pertence à Estação de Aquicultura e Hidrologia da Duke Energy International (A); o segundo, à piscicultura de Rolândia (B) e o terceiro, ao Programa de Repovoamento dos Rios do Paraná (C). Os dez primers para RAPD utilizados produziram 105 fragmentos polimórficos, conferindo um polimorfismo de 98,1% para os três estoques avaliados. A porcentagem de locos...
Palavras-chave: 5.05.04.00-2 Reprodução Animal divergência genética; Locos polimórficos; Peixe; RAPD; Reprodutores; Variabilidade genética. genetic divergence; Polymorphic loci; Fish; RAPD; Reproducers; Genetic variability.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4710
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Genetic monitoring by rapd markers for repopulation programs of Salminus brasiliensis (Pisces, Characiformes) - doi: 10.4025/actascianimsci.v35i2.15904 Animal Sciences
Almeida, Fernanda Simões de; Universidade Estadual de Londrina; Lopes, Carla Martins; Universidade Federal do Rio Grande do Sul; Orsi, Mário Luís; Universidade Estadual de Londrina; Sirol, Rodolfo Nardez; Companhia Paulista de Força e Luz; Sodré, Leda Maria Koelblinger; Universidade Estadual de Londrina.
The preservation of the genetic variability of hatchery stocks used to supplement natural populations is a priority. Current study employed RAPD markers to examine the genetic diversity of dourado samples from the middle Paranapanema river and from the broodstock used in the stock enhancement program of the Aquaculture and Hydrobiology Station at the Salto Grande Hydroelectric Power Plant. Nineteen RAPD primers were analyzed, which generated 299 bands and the latter were used for genetic analysis. The percentage of polymorphic fragments was higher in stock captured in the Paranapanema river than from fry stocks. The genetic diversity was lower in the broodstock than in natural population. Amova results showed that most inter-population genetic variation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.06.03.00-0 ou 2.02.04.00-0 dourado; Genetic variability; Hatchery stocks; Fry stocks Dourado; Genetic variability; Hatchery stocks; Fry stocks.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/15904
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Genetic diversity of Lippia rotundifolia Cham. in Minas Gerais, Brazil Biological Sciences
Meira, Messulan Rodrigues; Martins, Ernane Ronie; Resende, Luciane Vilela.
Lippia rotundifolia is an aromatic species, endemic from Campos rupestres and isolated by mountains. We aimed with the research to study the genetic variability of chá-de-pedestre (Lippia rotundifolia Cham.) with natural occurrence in ten places of the State of Minas Gerais, from molecular markers of ISSR type. The places of collection were: Parque Estadual de Serra Nova; Parque Estadual Veredas do Peruaçu; Abóboras community; Gigante community; edge of Rio do Peixe; environmental preservation area of Olhos d´água; private property in Joaquim Felício; Parque Estadual do Rio Preto; São Gonçalo do Rio das Pedras and brook of Rio Tigre. After the establishment of the extraction and amplification of DNA fragments with primers ISSR, an array of genetic distance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.02.03.00-4 pedestrian tea; Genetic variability; Medicinal plant; Conservation Genética Vegetal.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42754
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Allozyme analysis of the four species of Hypostomus (Teleostei: Loricariidae) from the Ivaí river, upper Paraná river basin, Brazil - doi: 10.4025/actascibiolsci.v35i4.16355 Biological Sciences
Paiva, Suzana de; Universidade Estadual de Maringá; Zawadzki, Claudio Henrique; Universidade Estadual de Maringa; Ruvulo-Takasusuki, Maria Claudia Colla; Universidade Estadual de Maringá; Lapenta, Ana Silvia; Universidade Estadual de Maringá; Renesto, Erasmo; Universidade Estadual de Maringá.
Allozyme electrophoresis analysis were performed in four species of Hypostomus (Loricariidae), H. albopunctatus, H. hermanni, H. regani, e Hypostomus sp. 1/NUP 5612 from the Ivaí river, a tributary of the upper Paraná river. The study of 14 loci revealed diagnostic characters and exclusive alleles in a low frequency. The heterozygosity ranged from 0.000 in H. albopunctatus to 0.199 in H. hermanni, which was higher than the heterozygosity in other samples of Hypostomus in literature, as well as in other fish groups. Hypostomus albopunctatus and H. regani revealed higher similarity (I = 0.804), while H. hermanni and Hypostomus sp. 1/NUP 5612 showed the least genetic identity (I = 0.569). All samples were genetically distinguished, despite there were several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.02.00.00-5 catfish; Heterozygosity; Neotropical fishes; Genetic variability; Siluriformes Genética.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/16355
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Molecular characterization of the invasive aquatic macrophyte Hydrilla verticillata (Hydrocharitaceae) in Brazil Anais da ABC (AABC)
LUCIO,LÉIA CAROLINA; THOMAZ,SIDINEI M.; PRIOLI,SÔNIA MARIA A.P.; BONI,TALGE A.; OLIVEIRA,ALESSANDRA V. DE; PRIOLI,ALBERTO JOSÉ.
Abstract: Invasive populations of macrophytes are widely distributed and have been successfully introduced and established in freshwater habitats. Hydrilla verticillata was first recorded in 2005 in the Upper Paraná River floodplain and in 2007 at the Itaipu Reservoir (Brazil-Paraguay border, ca. 300 km downstream from its first record). However, its genetic variability within different sites in South America is unknown. We used nucleotide sequences corresponding to the trnL-trnF fragment cpDNA to genetically characterize populations of H. verticillata in different ecosystems of the Upper Paraná River basin. The results indicated an absence of genetic differentiation within and between populations of the basin, and even individuals collected 600 km apart...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CpDNA; Genetic variability; TrnL-trnF; Upper Paraná River basin.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500623
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Genetic diversity assessed by microsatellite markers in the amphicarpic species Trifolium polymorphum Poir. Anais da ABC (AABC)
CONTERATO,IONARA F.; SCHIFINO-WITTMANN,MARIA TERESA; GUERRA,DIVANILDE; BÜTTOW,MIRIAM V.; AGNOL,MIGUEL DALL’; DAHMER,NAIR.
ABSTRACT Trifolium polymorphum Poir. is an amphicarpic forage legume from southern Brazil, Uruguay, Argentina, Paraguay and Chile. Information on the genetic diversity of natural populations in natural grasslands in southern Brazil is limited. In order to increase the knowledge about this species, an analysis of the genetic diversity was carried out in 10 natural populations of T. polymorphum with the use of 20 microsatellite markers. The expected heterozygosity in T. polymorphum populations ranged from 0.40 to 0.43, with a mean of 0.42. A total of 193 alleles were detected with a mean of 9.3 alleles per locus and polymorphic information content (PIC) for these markers of 0.62 to 0.89 with a mean of 0.84.The grouping based on the Jaccard’s coefficient of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Microsatellites; Natural grasslands; Pampa biome.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401685
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Genetic variation in native and farmed populations of Tambaqui (Colossoma macropomum) in the Brazilian Amazon: regional discrepancies in farming systems Anais da ABC (AABC)
Aguiar,Jonas; Schneider,Horacio; Gomes,Fátima; Carneiro,Jeferson; Santos,Simôni; Rodrigues,Luis R.; Sampaio,Iracilda.
The tambaqui, Colossoma macropomum, is the most popular fish species used for aquaculture in Brazil but there is no study comparing genetic variation among native and farmed populations of this species. In the present study, we analyzed DNA sequences of the mitochondrial DNA to evaluate the genetic diversity among two wild populations, a fry-producing breeding stock, and a sample of fish farm stocks, all from the region of Santarém, in the west of the Brazilian state of Pará. Similar levels of genetic diversity were found in all the samples and surprisingly the breeding stock showed expressive representation of the genetic diversity registered on wild populations. These results contrast considerably with those of the previous study of farmed stocks in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tambaqui; Mitochondrial DNA; Genetic variability; Aquaculture.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401439
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RAPD analysis revealing polymorphism in egg parasitoids of soybean stink bugs (Hemiptera: Pentatomidae) Anais da SEB
Aljanabi,Salah M.; Loiácono,Marta S.; Lourenço,Rodrigo T.; Borges,Miguel; Tigano,Myrian S..
The solitary egg parasitoids (Hymenoptera: Scelionidae) of Pentatomidae species complex are potential candidates for soybean (Glycine max L.) integrated pest management programs in Brazil. The correct identification of species and strains of these parasitoids is necessary to implement the use of biological control. Polymerase chain reaction (PCR) was used to generate random amplification of polymorphic DNA (RAPD) from male individuals of Telenomus podisi Ashmead and Trissolcus spp. collected in a soybean field in Brasília, Brazil in 1996. The analysis of 18 primers used to screen Trissolcus spp. individuals revealed different patterns related to the species analyzed. These results indicate the potential of RAPD markers to differentiate T. teretis Johnson,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Microhymenoptera parasitoids; Genetic variability; Molecular markers; RAPD.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000300009
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