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| Registros recuperados: 116 | |
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| Rivera-Jiménez, Hernando; Rossini, Bruno César; Tambarussi, Evandro Vagner; Ann Veasey, Elizabeth; Ibanes, Bruna; Marino, Celso Luis. |
| Growers appreciate Cattleya walkeriana and C. loddigesii due to striking shape and rarity. Thus, this study aimed to evaluate the feasibility of DNA barcode regions, namely ITS1, ITS2 and rpoC1, to discriminate between C. walkeriana and C. loddigesii species. DNA barcode regions were successfully amplified using primers designed to amplify plants. We also included sequences from public databases in order to test if these regions were able to discriminate C. walkeriana and C. loddigesii from other Cattleya species. These regions, and their combinations, demonstrated that the ITS1+ITS2 had the highest average interspecific distance (11.1%), followed by rpoC1 (1.06%). For species discrimination, ITS1+ITS2 provided the best results. The combined data set of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.00.00.00-6 orchid improvement; Genetic variation; Species separation. 2.02.03.00-4. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33024 |
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| Carneiro,Valdirene Leão; Bendicho,Maria Teresita; Santos,Rosalina Guedes; Casela,Marilda; Netto,Eduardo M.; Mota,Scarlet Torres Moraes; Pina,Iza Cristina Araújo; Nascimento,Roberto Meyer; Freire,Songeli Menezes; Barbosa,Theolis. |
| ABSTRACT Introduction Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG + 874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG + 874 A/T polymorphism on interferon-gamma test performance. Methods Patients diagnosed with pulmonary tuberculosis (75), volunteers with positive tuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no history of contact with tuberculosis (57) were evaluated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis infection; Genetic variation; Sensitivity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300202 |
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| Khatoon,Lubna; Khan,Inam Ullah; Shah,Shahid Ali; Jan,Muhammad Ishtiaq; Ullah,Farhat; Malik,Salman Akbar. |
| Malaria is one of the serious diseases threatening human health in Pakistan and contributes to a large proportion of the total malaria deaths in South Asia. However, little is known about the nature and extent of genetic diversity of the malarial parasites circulating in Pakistan. This study was designed to assess the infection status of Plasmodium and the genetic diversity of Plasmodium vivax and Plasmodium falciparum by analyzing msp-3α, msp-3β and msp-1, msp-2 genes respectively using allele specific nested PCR and RFLP assays. For this purpose, 130 field isolates were collected from the individuals who exhibited clinical symptoms associated with malaria in the Kohat region of Khyber Pakhtoonkhwa (KPK), Pakistan. Among 130 blood samples collected, P.... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Plasmodium vivax; Plasmodium falciparum; Genetic variation; Pakistan. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200014 |
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| Tang,Y.L.; Lu,J.H.; Cao,L.; Wu,M.H.; Peng,S.P.; Zhou,H.D.; Huang,C.; Yang,Y.X.; Zhou,Y.H.; Chen,Q.; Li,X.L.; Zhou,M.; Li,G.Y.. |
| To find Epstein-Barr virus (EBV) strains with genetic variations of EBV latent membrane protein 1 (EBV-LMP1) from nasopharyngeal carcinoma (NPC), the full-length DNA of LMP1 genes from 21 NPC biopsies obtained in Hunan province in southern China was amplified and sequenced. Our sequences were compared to those previously reported by the Clustal V method. Results showed that all 21 sequences displayed two amino acid changes most frequently in LMP1 of CD4+ T cell epitopes at codons 144 (F®I, 21/21) and 212 (G®S, 19/21) or (G®N, 2/21). We also show that type A EBV strain is prevalent in the cases of NPC from Hunan province with a 30-bp 18/21 deletion, and we highlight that this deletion resulted in loss of one of the CD4+ T cell-restricted epitopes. The other... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variation; Epitope; Immune recognition; Latent membrane protein 1; Tumorigenicity. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200006 |
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| Lemaire,D.; Barbosa,T.; Rihet,P.. |
| Vaccine development faces major difficulties partly because of genetic variation in both infectious organisms and humans. This causes antigenic variation in infectious agents and a high interindividual variability in the human response to the vaccine. The exponential growth of genome sequence information has induced a shift from conventional culture-based to genome-based vaccinology, and allows the tackling of challenges in vaccine development due to pathogen genetic variability. Additionally, recent advances in immunogenetics and genomics should help in the understanding of the influence of genetic factors on the interindividual and interpopulation variations in immune responses to vaccines, and could be useful for developing new vaccine strategies.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human vaccines; Vaccinomics; Genetic variation; Immunization. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500001 |
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| Dulgheroff,Ana Carolina Bernardes; Silva,George Allan Villarouco da; Naveca,Felipe Gomes; Oliveira,Adriana Gonçalves de; Domingues,André Luiz da Silva. |
| ABSTRACT Group A rotaviruses are the main causative agent of infantile gastroenteritis. The segmented nature of the viral genome allows reassortment of genome segments, which can generate genetic variants. In this study, we characterized the diversity of the VP7, VP4 (VP8*), VP6, NSP4, and NSP5 genes of the rotaviruses that circulated from 2005 to 2011 in the Triângulo Mineiro (TM) region of Brazil. Samples with genotypes G2 (sublineages IVa-1 and IVa-3), G1 (sublineage I-A), G9 (lineage III), G12 (lineages II and III), G8 (lineage II), G3 (lineage III), P[4] (sublineages IVa and IVb), P[8] (sublineages P[8]-3.6, P[8]-3.3, and P[8]-3.1), I2 (lineage VII), E2 (lineages VI, XII, and X), and H2 (lineage III) were identified. The associations found in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Infantile diarrhea; Genotype; Genetic variation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300731 |
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| Registros recuperados: 116 | |
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