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| Registros recuperados: 135 | |
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| Vieira, Marcelo de Andrade; UFPR; Camargo, Mariana Krusquevis; UEPG; Daros, Edelclaiton; UFPR; Zagonel, Jeferson; UEPG; Koehler, Henrique Soares; UFPR. |
| O produtor de milho verde busca a profissionalização de sua produção, a fim de fornecer produtos de alta qualidade e em quantidade suficiente para suprir os mercados mais exigentes. Para se alcançar esse objetivo, devem ser adotadas técnicas de manejo que proporcionem altas produtividades sem que sejam afetadas negativamente as características comerciais. Com o objetivo de avaliar a aptidão de cultivares e o efeito da população de plantas na produção de milho verde, foi conduzido um experimento em Ponta Grossa, Estado do Paraná. Adotou-se o delineamento de blocos ao acaso, em esquema de parcelas subdivididas, sendo os tratamentos principais as cultivares (Penta, 30P34, DKB 214 e SWB 551) e os secundários as populações de plantas (3,5; 5,0; 6,5; 8,0 e 9,5... |
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Palavras-chave: 5.01.00.00-9 Agronomia Zea mays L.; Densidade de semeadura; Genótipo; Milho verde Manejo e tratos culturais Zea mays L.; Seedling density; Genotype; Green corn. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/987 |
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| Levy, Ricardo Michael; UEL; Homechin, Martin; UEL; Santiago, Débora Cristina; UEL; Cadioli, Marina Capparelli; UEL; Baida, Fernando César; UEL. |
| O uso de híbridos e genótipos de milho resistentes aos nematóides formadores de galhas em sistemas de rotação de culturas mantém sua população em níveis baixos, diminuindo as perdas e possibilitando, posteriormente, o uso de genótipos mais suscetíveis. No presente trabalho, foi avaliada a resistência de 18 genótipos de milho ao parasitismo de M. paranaensis e a M. incógnita raça 1 em casa-de-vegetação. As plantas foram inoculadas com 5.000 ovos e eventuais juvenis dos respectivos nematóides. O delineamento utilizado foi inteiramente ao acaso, com dez repetições para cada tratamento com os nematóides e cinco repetições para a testemunha sem inoculação. Também foi realizada a técnica de coloração das raízes com fucsina ácida, pelo método de Byrd et al.... |
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Palavras-chave: 5.01.02.00-1 Fitossanidade Zea mayz; Nematóide; Resistência; Genótipo Zea may; Nematode; Resistance; Genotype. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/761 |
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| Seidler-Łożykowska, Katarzyna; Institute of Natural Fibres and Medicinal Plants; Kuczyńska, Anetta; Polish Academy of Sciences; Mikołajczyk, Katarzyna; Plant Breeding and Acclimatization Institute; Nowakowska, Joanna; Plant Breeding and Acclimatization Institute; Bocianowski, Jan; Poznań University of Life Sciences. |
| In order to estimate genetic diversity among starting materials and breeding strains of caraway, a collection of 17 accessions from botanical gardens in Europe, two cultivars ‘Rekord’ and ‘Kończewicki’, and four own breeding strains were analyzed by RAPD-PCR. The representative samples, each of five individual plants of accession, cultivar or strain, were taken from young rosette leaves. Forty of Genset Oligos RAPD primers were used for analysis and eight of them produced clear and reproducible banding patterns. In total, 62 banding patterns were obtained revealing 23 polymorphic bands, whereas the number of polymorphic bands ranged from two to four for one primer. The GS12 and GS43 primers generated two polymorphic bands, while each of the GS8, GS21,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carum carvi L.; Genetic distance; Genotype; RAPD. |
| Ano: 2014 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18197 |
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| Andrade, Carlos Alberto de Bastos; UEM; Scapim, Carlos Alberto; UEM; Braccini, Alessandro de Lucca e; UEM; Martorelli, Danilo Takara; UEM. |
| Com o objetivo de caracterizar o crescimento e a produtividade de grãos, das cultivares ‘Ouro Negro’ e ‘BRS MG Talismã’, em condições de campo, na Estação Experimental de Coimbra, Estado de Minas Gerais, pertencente à Universidade Federal de Viçosa (UFV), experimentos de campo foram conduzidos em que se avaliaram o crescimento vegetativo e a produtividade de grãos das duas cultivares. Foram efetuadas amostragens aos 12, 22, 36, 51, 65 e 76 dias após emergência (DAE), sendo determinadas a área foliar e a biomassa dos diferentes componentes da parte aérea, além do índice de área foliar e das taxas de crescimento absoluto e assimilatórias líquidas. Aos 80 DAE (colheita), foram determinados a produtividade de grãos e os seus componentes (número de plantas por... |
| Tipo: Pesquisa no campo |
Palavras-chave: 5.01.03.04-0 Produção de Mudas Phaseolus vulgaris; Genótipos; Análise de crescimento; Componentes da produção; Produtividade de grãos. Fitotecnia Phaseolus vulgaris; Genotype; Growth analysis; Production components; Grain yield. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6397 |
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| Inostroza,Luis; del Pozo,Alejandro; Matus,Iván; Hayes,Patrick. |
| Grain yield (GY) and drought tolerance of recombinant chromosome substitution lines (RCSLs) derived from the cross between Hordeum vulgareL.subsp. spontaneum (K. Koch) Thell. and H. vulgare L. subsp. vulgare cv. Harrington, were studied in two contrasting environments, one with water stress (WWS) and the other with no water stress (NWS), during three growing seasons, 2004-2005, 2005-2006 and 2006-2007. In the first season 80 RCSLs were sowed and in the following a selection of 13 RCSLs. An a-lattice design was used in all the experiments. With the data of GY obtained in sites WWS and NWS it was calculated the drought sensitivity index (DSI). During 2004-2005 growing season GY varied greatly among locations, reflecting differences in water availability. The... |
| Tipo: Journal article |
Palavras-chave: Barley; Drought sensitivity index; Grain yield water stress; Genotype. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072007000300004 |
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| Milosevic,Tomo; Milosevic,Nebojsa. |
| In situ selection of vineyard peach (Prunus pérsica spp. vulgaris Mill.) genotypes was conducted in 2006 and 2007 in the region of Kfusevac (Central Serbia). Identification, observation and recording of the phenological and pomological properties of the highest-quality genotypes were carried out. Twenty-eight genotypes of the highest quality were selected out of 2093 genotypes, based on biological and pomological properties, and compared to those of cv. Redhaven (control). The onset of flowering varied (April 7-21), being early in two genotypes and late in one genotype, as compared to Redhaven, while 25 genotypes showed an intermediate flowering date, as Redhaven did. Campanula flowers were found in six genotypes, as in Redhaven, and the rosaceous type in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breeding; Flowering; Fruit and stone; Genetic diversity; Genotype. |
| Ano: 2010 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952010000300005 |
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| MORAES,GREICE G.; REUTER,CÉZANE P.; RENNER,JANE D.P.; KLINGER,ELISA I.; FERREIRA,MICHELE B.; MELLO,ELZA D. DE; VALIM,ANDRÉIA DE M.; BURGOS,MIRIA S.. |
| ABSTRACT Background: Children and adolescents with at-risk genotypes (AA/AT) of the rs9939609 polymorphism in FTO, a fat mass and obesity-associated gene, may exhibit different cardiometabolic profile responses than subjects with the TT genotype after an interdisciplinary intervention. Methods: The sample consisted of 36 school children from southern Brazil. We used DNA quantitation and real-time polymerase chain reaction (PCR) for polymorphism genotyping. We measured anthropometric parameters (body mass index (BMI), waist circumference, hip circumference, waist-hip ratio, body fat percentage and skinfold sum), biochemical parameters (glucose, lipid profile, ultra-sensitive C-reactive protein, uric acid, alanine aminotransferase, aspartate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotype; Intervention; Obesity; Children; Physical activity. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000602331 |
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| Del Castilho,C.C.; Santos,T.T; Rodrigues,C.A.F.; Torres Filho,R.A.. |
| The purpose of this work was to evaluate the effect of genotype and sex on the performance characteristics (weight gain, feed conversion and livability) and yields (carcass, breast and legs) of six free-range genotypes: Pesadão Vermelho (GEN1), Carijó (GEN2), Pescoço Pelado 1 (GEN3), Pescoço Pelado 2 (GEN4), Pescoço Pelado 3 (GEN5) and Pescoço Pelado 4 (GEN6). A total of 1584 sexed one day old chicks (792 males and 792 females) were housed in 48 pens, 33 birds per pen. The experimental design was a completely randomized factorial 2 x 6 (six genotypes and two sexes) design, with four replicates each. Diets were based on corn and soybean meal (no animal protein) provided in a feeding program in four phases: pre-starter (1 to 21 days), starter (22 to 42... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alternative aviculture; Genotype; Performance; Yields. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500029 |
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| Freire,K.M.B.; Silva,C.C.; Duarte,S.S.M.; Teodoro,L.S.; Costa,E.O.A.; Silva,D.C.; Godoy,F.R.; da Cruz,A.D.; da Cruz,A.S.. |
| RESUMO O objetivo desta pesquisa foi avaliar os SNPs rs471462296, rs456245081 e rs438495570 do gene DGAT1 em bovinos Nelore. Foram analisados 109 bovinos. A extração do DNA genômico foi realizada do sangue dos animais, usando-se o kit Ilustra Blood Genomic Prep Mini Spin® (GE Healthcare, UK). A concentração e o grau de pureza do DNA foram determinados por meio de espectrofotômetro (Nanodrop - Thermo Fisher Scientifc, USA). A genotipagem dos SNPs ocorreu mediante o emprego do ensaio Taqman® (Applied Biosystems, USA). Na análise genômica, não foram encontradas alterações nas frequências alélicas e genotípicas (P≥0,05) para os SNPs testados. Dessa forma, a região 5’UTR analisada apresentou-se monomórfica e a variação de SNPs não foi observada, o que limita... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bovine; Phenotype; Genotype; Selection. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200720 |
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| Zucatto,A.S.; Aquino,M.C.C.; Inácio,S.V.; Figueiredo,R.N.; Pierucci,J.C.; Perri,S.H.V.; Meireles,M.V.; Bresciani,K.D.S.. |
| Considering the proximity of sheep farmers to animals that are possibly diseased or releasing fecal oocysts into the environment and the marked pathogenicity in lambs, the aim of this study was to determine the occurrence and to molecularly characterize the infection by Cryptosporidium spp. in lambs in the South Central region of the state of São Paulo, Brazil. A total of 193 fecal samples were collected from sheep of several breeds, males and females, aged up to one year. Polymerase chain reaction (nested-PCR) was used to amplify DNA fragments from the subunit 18S rRNA gene and indicated 15% positivity; sequencing of amplified fragments was possible for 19 samples. Analysis of the obtained sequences showed that the identified species were Cryptosporidium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptosporidiosis; Sheep; Nested-PCR; Genotype. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200441 |
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| VARELA,NELSON M; QUIÑONES,LUIS A; ORELLANA,MYRIAM; PONIACHIK,JAIME; CSENDES,ATTILA; SMOK,GLADYS; RODRIGO,RAMÓN; CÁCERES,DANTE D; VIDELA,LUIS A. |
| CYP2E1 enzyme is related to nonalcoholic steatohepatitis (NASH) due to its ability for reactive oxygen species production, which can be influenced by polymorphisms in the gene. The aim of this study was to investigate hepatic levels, activity, and polymorphisms of the CYP2E1 gene to correlate it with clinical and histological features in 48 female obese NASH patients. Subjects were divided into three groups: (i) normal; (ii) steatosis; and (iii) steatohepatitis. CYP2E1 protein level was assayed in microsomes from liver biopsies, and in vivo chlorzoxazone hydroxylation was determined by HPLC. Genomic DNA was isolated for genotype analysis through PCR. The results showed that liver CYP2E1 content was significantly higher in the steatohepatitis (45%; p=0.024)... |
| Tipo: Journal article |
Palavras-chave: Steatohepatitis; NASH; CYP2E1; Obesity; Genotype; Chlorzoxazone. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100010 |
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| Saleh,Basel. |
| The goal of this study was to investigate the molecular characterization of A. macrostachyum genotypes grown in the Western coast of Syria using RAPD and ISSR techniques. PCR amplifications with 20 RAPD primers gave an average of 9.25 selected markers/ primer, with a maximum of 17 (OPG05) and a minimum of four (OPA02). Percentage of polymorphic bands ranged from 40 to 100% according to RAPD primers tested. Among the 185 selected bands, 160 (84.96%) were polymorphic. The amplification with seven ISSR primers generated 88 bands, and 80 (90.91%) were polymorphic. (CA)8RG and (AG)8GTG ISSR primers tested in this study yielded highly informative patterns. RAPD and ISSR fragment sizes ranged from 0.2-3 kb. Based on this study, the use of RAPD and ISSR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: A. macrostachyum; Genetic diversity; Genotype; ISSR; Polymorphism; RAPD. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500002 |
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| Diaz,Ricardo Sobhie; Sucupira,Maria Cecilia A; Vergara,Tania RC; Brites,Carlos; Bianco,Rosana Del; Bonasser Filho,Francisco; Colares,Geova Keny B; Portela,Estevão; Cherman,Lia Adler; Barcelos,Nemora Tregnago; Tupinambas,Unai; Turcato Jr,Gilberto; Allamasey,Lisa; Bacheler,Lee; Tuohy,Martin. |
| OBJECTIVE: To investigates how the use of HIV-1 resistance tests influences physician decision-making. METHODS: Ten experienced reference physicians from the Brazilian Network for Drug Resistance each received ten patients' case histories. The selected patients had experienced at least two virological failures. First, reference physicians were asked to empirically select a new regimen for each patient. Second, after genotype report (ViroSeq 2.6) was provided, and physicians were again asked to select a new regimen considering this additional information. Finally, they were asked to select a regimen after receiving a virtual phenotype result (vircoTYPE 3.9.00). RESULTS: In 79% of the cases, physicians changed their empirical choice of regimen after... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotype; Virtual phenotype; Antiretroviral resistance; Brazil. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500011 |
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| Codes,L.; Freitas,L.A.R. de; Santos- Jesus,R.; Vitvitski,L.; Silva,L.K.; Trepo,C.; Reis,M.G.; Paraná,R.. |
| Hepatitis C virus displays a high degree of genetic mutation, with considerable heterogeneity, motivating clinical and biomolecular investigations. It is necessary to understand the effects of genotypes on the course of the disease, as well as their peculiarities at the regional level. OBJECTIVE: The study objective was to compare epidemiological, biochemical and histological aspects of hepatitis C virus genotypes 1 and 3 in Salvador, Bahia. STUDY DESIGN: Data were collected retrospectively from outpatient medical records. MATERIALS AND METHODS: 127 patients with positive anti-HCV results were selected, based on detectable RNA-HCV (RT-PCR) of genotypes 1a, 1b and 3a. RESULTS: Thirty-nine (30.7%) individuals were infected by subtype 1a, 45 (35.4%) by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis C; Genotype. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000600009 |
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| Serravalle,Karina; Santos,Norma; Sardi,Silvia Inês; Silva,Sarah Peregrino Santos; Ribeiro Junior,Hugo da Costa; Mattos,Ângela Peixoto de; Campos,Gúbio Soares. |
| Rotavirus is a major cause of infectious diarrhea in infants and young children. The objective of this study was to characterize the genotypes of Human Rotavirus found in children hospitalized with acute diarrhea in the Pediatric Hospital Prof. Hosannah de Oliveira of the UFBA in Salvador, Bahia, Brazil, during the years of 1999, 2000 and 2002. Fecal samples were analyzed (n=358) by methods EIARA and SDS-PAGE for detection of Rotavirus. Positive samples of one or two of these methods (n=168) were submitted to RT-PCR and Multiplex-Nested PCR to determine genotypes G and P. A hundred sixty-eight (46.9%) samples were positive and 190 (53.1%) negative. Only 17 (4.7%) samples had divergent results. The distribution of genotypes G during the first year, showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; PCR; Genotype; Children. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100010 |
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| Focaccia,R.; Baraldo,D.C.M.; Ferraz,M.L.G.; Martinelli,A.L.C.; Carrilho,F.J.; Gonçales Jr.,F.L.; Pedroso,M.L.A.; Coelho,H.S.M.; Lacerda,M.A.; Brandão,C.E.; Mattos,A.A.; Lira,L.G.C.; Zamin Jr.,I.; Pinheiro,J.O.P.; Tovo,C.V.; Both,C.T.; Soares,J.A.S.; Dittrich,S.. |
| Hepatitis C virus (HCV) infection is a serious public health problem, since 80% to 85% of HCV carriers develop a persistent infection that can progress into liver cirrhosis and hepatocarcinoma. Considering that the response of hepatitis C patients to combination therapy with interferon and ribavirin depends on HCV characteristics as well as on host features, we made a retrospective analysis of demographic and anthropometrical data and HCV genotype distribution of chronic hepatitis C patients treated in public and private reference centers in Brazil. The medical records of 4,996 patients were reviewed, 81% from public and 19% from private institutions. Patients' median age was 46 years, and there was a higher prevalence of male (62%) and white patients... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HCV; Chronic hepatitis C; Genotype; Body weight; Brazil. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500003 |
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| Toledo,Paula Virginia Michelon; Carvalho,Denise Siqueira de; Romagnoli,Luiza; Marcinko,Gustavo; Cunha,Clovis Arns da; Souza,Margely Nunes de; Brindeiro,Rodrigo; Queiroz-Telles,Flávio de. |
| Antiretroviral therapy (ART) has reduced morbidity and mortality related to human immunodeficiency virus (HIV) infection, but in spite of this advance, HIV mutations decrease antiretroviral susceptibility, thus contributing to treatment failure in patients. Genotyping HIV-1 allows the selection of new drugs after initial drug failure. This study evaluated the genotypic profile of HIV-1 isolates from treated (drug-experienced) patients in Paraná, Brazil. The prevalence of mutations in reverse transcriptase (RT) and protease (PR) genes were assessed. We analyzed 467 genotypes of patients with HIV-1 viral loads above 1,000 copies/mL. Mutations at HIV-1 RT and PR genes and previously used ART regimens were recorded. The most prevalent RT mutations were: 184V... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Genotype; Antiretrovirals; Drug-experienced patients. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400009 |
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| Registros recuperados: 135 | |
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