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| Registros recuperados: 56 | |
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| Scali,Monica; Vignani,Rita; Bigliazzi,Jacopo; Paolucci,Elisa; Bernini,Andrea; Spiga,Ottavia; Niccolai,Neri; Cresti,Mauro. |
| Background: Cinta Senese (CS) is an autochthonous Tuscan breed, which risked extinction since the 60s. Results: Monitoring the genetic variability of the actual population by use of DNA molecular markers is essential to address a correct breeding policy, finalized to obtain the race preservation and its fitness in the future. 17 SSRs autosomal markers and 1 associated to the X chromosome were used to genotype 86 individuals belonging to the CS and 12 belonging to two main white races Landrace (L), Large White (LW) and crosses between LW and L and L and CS widespread in Tuscany and used in the recent past to obtain hybrids with the CS. Conclusions: A dendrogram of similarity measures the relative genetic distance between individuals in the population. Data... |
| Tipo: Journal article |
Palavras-chave: Cinta Senese; Genetic diversity; Genotyping; Microsatellites; Sus scrofa. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200001 |
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| Arias,Renée S; Stetina,Salliana R; Scheffler,Brian E. |
| Currently, a large number of microsatellites are available for Rotylenchulus reniformis (reniform nematode); however, two barriers exist for genotyping samples from different geographical areas. The limited amount of nucleic acids obtained from single nematodes which would require their multiplication to obtain enough DNA for testing; and the strictly regulated transport of live samples and multiplication in greenhouse for being a plant pathogen. Whole-genome amplification (WGA) of samples consisting of one and five dead gravid females with their associated egg masses was successfully performed on disrupted tissue using three commercial kits. DNA yield after WGA ranged from 0.5 to 8 µg and was used to test 96 microsatellite markers we previously developed... |
| Tipo: Journal article |
Palavras-chave: Genotyping; Reniform nematode; SSR; STR; WGA. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300014 |
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| Picazo,María G; García-Olmo,Dolores C. |
| Background Genotyping of mice is a common procedure in animal facilities. The aim of this study was to compare the quantity and quality of DNA extracted from samples obtained from young mice (YM; 10 d old) and adult mice (AM; 12 weeks old). We collected samples from the tail and ear of YM and AM. We also sampled blood, check cells (via buccal swabs), hair and fecal pellets of AM, and biopsied distal phalanx of YM. We isolated DNA using commercial kits and determined concentrations and purity by spectrophotometry. The integrity of DNA was evaluated by agarose-gel electrophoresis and polymerase chain reaction (PCR). Results DNA in all samples was amplified successfully but the intensities of bands after electrophoresis was heterogeneous. In general, tissues... |
| Tipo: Journal article |
Palavras-chave: DNA extraction; Genotyping; Mouse. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200004 |
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| Njung'e,Vincent; Deshpande,Santosh; Siambi,Moses; Jones,Richard; Silim,Said; De Villiers,Santie. |
| Background: Pigeonpea (Cajanus cajan (L.) Millsp.) is a drought tolerant legume of the Fabaceae family and the only cultivated species in the genus Cajanus. It is mainly cultivated in the semi-arid tropics of Asia and Oceania, Africa and America. In Malawi, it is grown as a source of food and income and for soil improvement in intercropping systems. However, varietal contamination due to natural outcrossing causes significant quality reduction and yield losses. In this study, 48 polymorphic SSR markers were used to assess the diversity among all pigeonpea varieties cultivated in Malawi to determine if a genetic fingerprint could be identified to distinguish the popular varieties. Results: A total of 212 alleles were observed with an average of 5.58 alleles... |
| Tipo: Journal article |
Palavras-chave: Genetic variation; Genotyping; Microsatellites; Seed purity. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300008 |
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| Jacobs,Clifford; Pearce,Brendon; Du Plessis,Mornè; Hoosain,Nisreen; Benjeddou,Mongi. |
| Human organic cation transporter 1 is primarily expressed in hepatocytes and mediates the electrogenic transport of various endogenous and exogenous compounds, including clinically important drugs. Genetic polymorphisms in the gene coding for human organic cation transporter 1, SLC22A1, are increasingly being recognized as a possible mechanism explaining the variable response to clinical drugs, which are substrates for this transporter. The genotypic and allelic distributions of 19 nonsynonymous and one intronic SLC22A1 single nucleotide polymorphisms were determined in 148 healthy Xhosa participants from South Africa, using a SNAPshot® multiplex assay. In addition, haplotype structure for SLC22A1 was inferred from the genotypic data. The minor allele... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymorphism; Haplotype; Population genetic structure; Genotyping; Genetic variability. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300006 |
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| Zhong,Zhixiong; Wu,Heming; Wu,Hesen; Zhao,Pingsen. |
| Abstract There is currently no data about the genetic variations of APOE in Hakka population in China. The aim of this study was to analyze the allelic and genotypic frequencies of APOE gene polymorphisms in a large ethnic Hakka population in southern China. The APOE genes of 6,907 subjects were genotyped by the gene chip platform. The allele and genotype frequencies were analyzed. Results showed that the ∊3 allele had the greatest frequency (0.804) followed by ∊2 (0.102), and ∊4 (0.094), while genotype ∊3/∊3 accounted for 65.43% followed by ∊2/∊3 (15.85%), ∊3/∊4 (14.13%), ∊2/∊4 (3.01%), ∊4/∊4 (0.84%), and ∊2/∊2 (0.74%) in all subjects. The frequencies of the ∊4 allele in Chinese populations were lower than Mongolian and Javanese, while the frequencies of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apolipoprotein E; Genetic polymorphism; Hakka; Southern China; Genotyping. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500742 |
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| Luz,José Magno Queiroz; Machado,Daniel Lucas Magalhães; Maciel,Gabriel Mascarenhas; Freitas,Joelson André de; Oliveira,Roberta Camargos de. |
| ABSTRACT The commercial use of onion hybrids is preferred by producers. In contrast, the production of hybrid onion seeds is extremely inefficient. This is due to the use of lineages obtained by successive self-fertilizations assuming the effect of inbreeding depression per se. Therefore, it is necessary to understand new alternatives to reduce the effect of inbreeding depression in the strains. The objective of this study was to evaluate possible differences in polymorphism and levels of heterozygosity of strains obtained from intercrossed and self-fertilized plants. Twelve onion populations belonging to Bayer’s breeding program (Granex, IPA-1, IPA-2 and IPA-3) were used, obtained by self-fertilization of one plant, intercrossing of two plants or... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium cepa; Hybrid; Onion polymorphism; Genotyping; Primers. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000300274 |
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| CAMPOS, T. de; AZÊVEDO, H. S. F. da S.; OLIVEIRA, J. C. de; FERREIRA FILHO, J. A.; YOMURA, R. B. T.; SILVA, L. M. da. |
| A pecuária na Amazônia representa uma cadeia produtiva importante para pequenos, médios e grandes produtores, uma vez que mais de 80% das áreas desmatadas são ocupadas por pastagens e cerca da metade delas encontra-se degradada ou em vias de degradação. A utilização de pastagens consorciadas pode ser uma alternativa de recuperação, manutenção da produtividade por um período maior e redução do tempo dos animais no pasto. Essa tecnologia tem sido amplamente defendida e utilizada pelos pecuaristas. A utilização do amendoim forrageiro em consórcio com gramíneas em pastagens tem mostrado resultados bastante significativos em relação ao aumento na rentabilidade e melhoria das condições do pasto. No entanto, ainda há baixa oferta de cultivares no mercado. No... |
| Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) |
Palavras-chave: Amendoim forrageiro; Forage peanut; Cacahuete forrajero; Marcador microssatélite; Genotipagem; Fitomejoramiento; Marcadores genéticos.; Genotipificación; Híbridos; Leguminosas forrajeras; Melhoramento genético vegetal; Leguminosa forrageira; Marcador molecular; Híbrido; Plant breeding; Forage legumes; Genetic markers; Hybrids; Genotyping; Arachis pintoi.. |
| Ano: 2016 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1064915 |
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| Benhaim, David; Guyomard, Rene; Chatain, Beatrice; Quillet, Edwige; Begout, Marie-laure. |
| Because captivity constitutes a drastic environmental change, domestication is expected to induce a rapid genetic selection for behavioural traits. In this study, we searched for genetic differences in behaviour among brown trout juveniles from two strains differing for their domestication history, i.e. an almost pure native wild Mediterranean population (W) and an Atlantic domesticated strain (D). In order to assess pure genetic differences among strains, males from the two origins were mated with Mediterranean females to produce two experimental crosses (WW and WD). The swimming activity characteristics of individual WW and WD juveniles were compared before and after the application of a stress (light switched off suddenly, followed by a 5-min period of... |
| Tipo: Text |
Palavras-chave: Domestication; Genotyping; Microsatellite; Swimming activity; Restocking. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00152/26301/24409.pdf |
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| Rebelato,Arnaldo Basso; Caetano,Alexandre Rodrigues. |
| Abstract: Runs of homozygosity (ROHs) are long stretches of homozygous genomic segments, identifiable by molecular markers, which can provide genomic information for accurate estimates to characterize populations, determine evolutionary history and demographic information, estimate levels of consanguinity, and identify selection signatures in production animals. This review paper aims to perform a survey of the works on the efficiency of ROHs for these purposes. Factors such as genetic drift, natural or artificial selection, founder effect, and effective population size directly influence the size and distribution of ROHs along the genome. Individually, genome estimates of consanguinity based on ROHs can be obtained using the FROH index, which is generally... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Autozygosity; FROH; Genotyping; Selection signatures; Single nucleotide polymorphism; SNPs. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000900975 |
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| Borba,Tereza Cristina de Oliveira; Mendes,Clistiane dos Anjos; Guimarães,Élcio Perpétuo; Brunes,Tuliana Oliveira; Fonseca,Jaime Roberto; Brondani,Rosana Vianello; Brondani,Claudio. |
| The objective of this study was to evaluate the genetic variability of rice (Oryza sativa) landraces collected in Brazilian small farms. Twelve simple sequence repeat (SSR) markers characterized 417 landraces collected in 1986, 1987 and 2003, in the state of Goiás, Brazil. The number of landraces with long and thin grain type increased in the evaluated period, probably due to market demand. Based on the molecular data, the genetic variability increased during this period and, as per to the factorial correspondence analysis, most of the accessions were grouped according to the year of collection. The incorporation of modern rice cultivars in landrace cultivation areas and the selection carried out by small farmers are the most probable factors responsible... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oryza sativa; Genotyping; Microsatellites; Participatory breeding; Rice breeding. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000700009 |
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| Gonçalves,Aline Najara Domingos; Soares,Cleber Oliveira; Sanches,Simone Camargo; Reis,Fernando Alvarenga; Rosinha,Grácia Maria Soares. |
| Abstract: The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotyping; PRNP gene; Single nucleotide polymorphisms; Transmissible spongiform encephalopathies.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500684 |
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| Silva,EN. |
| The infectious bronchitis virus (IBV) was detected for the first time in Brazil by Hipólito in 1957 in chickens sold life in the municipal market of Belo Horizonte, MG, when commercial poultry production was just starting in that country. The Massachusetts (Mass) serotype was identified. However, the clinical disease was only observed in 1975, when poultry production was intensely growing. The extensive outbreak produced the classical condition in layers and breeders, affecting egg production and quality, whereas broilers presented respiratory and "nephritis-nephrosis" signs. The disease rapidly spread to all poultry-producing regions in the country, and in 1979, both the imports and the manufacturing of live vaccines against IB strains Mass, H120 and H52,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; Genotyping; Infectious Bronchitis; PCR; Vaccination; Variants. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000300009 |
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| Abreu,JT; Mourão,MM; Santos,CE; Veloso,CJM; Resende,JS; Flatschart,RB; Folgueras-Flatschart,AV; Júnior,SN; Santoro,MM; Mendes,ACR; Franco,GR; Silva,A; Campos,AB; Fernandez,S. |
| Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious bronchitis virus; Genotyping; Spike glycoprotein; Nucleocapsid; Phylogenetic tree. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200005 |
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| Soares,Maria Amélia Menck; Rodrigues,Marcelo Teixeira; Mognol,Giuliana Patrícia; Ribeiro,Lucinéia de Fátima Chasko; Silva,José Luis da Conceição; Brancalhão,Rose Meire Costa. |
| Three different regions of the alpha s1-casein gene (CSN1S1) were investigated to determine the frequencies of major alleles for null, low, intermediate and high milk protein expression in a herd of dairy goats raised in the southeastern region of Brazil. Genomic DNA samples were obtained from leukocytes of 145 dairy goats and regions of interest in the gene were amplified through Polymerase Chain Reaction (PCR), then evaluated in both agarose (O and E allele) and polyacrylamide gels (F allele). For better characterization of the F allele, a PCR-RFLP (PCR-Restriction Fragment Length Polymorphism) study was performed employing the endonuclease XmnI. The allelic frequencies in the herd of 62 Saanen goats studied were: CSN1S1E = 0.35; CSN1S1F = 0.30; CSN1S1O1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alpine; Capra hircus; CSN1S1; Genotyping; Milk proteins; Saanen. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000600008 |
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| Registros recuperados: 56 | |
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