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Solubilization of insoluble inorganic phosphate by Burkholderia cepacia DA23 isolated from cultivated soil BJM
Song,Ok-Ryul; Lee,Seung-Jin; Lee,Yong-Seok; Lee,Sang-Cheol; Kim,Keun-Ki; Choi,Yong-Lark.
A mineral phosphate solubilizing bacterium, Burkholderia cepacia DA23 has been isolated from cultivated soils. Phosphate-solubilizing activities of the strain against three types of insoluble phosphate were quantitatively determined. When 3% of glucose concentration was used for carbon source, the strain had a marked mineral phosphate-solubilizing activity. Mineral phosphate solubilization was directly related to the pH drop by the strain. Analysis of the culture medium by high pressure liquid chromatography identified gluconic acid as the main organic acid released by Burkholderia cepacia DA23. Gluconic acid production was apparently the result of the glucose dehydrogenase activity and glucose dehydrogenase was affected by phosphate regulation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insoluble phosphate; Gluconic acid; Burkholderia cepacia; Glucose dehydrogenase.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100030
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Initial pH of medium affects organic acids production but do not affect phosphate solubilization BJM
Marra,Leandro M.; Oliveira-Longatti,Silvia M. de; Soares,Cláudio R.F.S.; Lima,José M. de; Olivares,Fabio L.; Moreira,Fatima M.S..
The pH of the culture medium directly influences the growth of microorganisms and the chemical processes that they perform. The aim of this study was to assess the influence of the initial pH of the culture medium on the production of 11 low-molecular-weight organic acids and on the solubilization of calcium phosphate by bacteria in growth medium (NBRIP). The following strains isolated from cowpea nodules were studied: UFLA03-08 (Rhizobium tropici), UFLA03-09 (Acinetobacter sp.), UFLA03-10 (Paenibacillus kribbensis), UFLA03-106 (Paenibacillus kribbensis) and UFLA03-116 (Paenibacillus sp.). The strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO4)2 in liquid medium regardless of the initial pH, although without a significant difference...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chelation; Propionic acid; Gluconic acid; Tartaric acid; Malic acid; Tropical soils.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200367
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Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors BJPS
Silva,Aline Ramos da; Tomotani,Ester Junko; Vitolo,Michele.
Conversion of sucrose into fructose and gluconic acid using invertase, glucose oxidase and catalase was studied by discontinuous (sequential or simultaneous addition of the enzymes) and continuous (simultaneous addition of the enzymes in a 100 kDa-ultrafiltration membrane reactor) processes. The following parameters were varied: concentration of enzymes, initial concentration of substrates (sucrose and glucose), pH, temperature and feeding rate (for continuous process). The highest yield of conversion (100%) was attained through the discontinuous (batch) process carried out at pH 4.5 and 37 ºC by the sequential addition of invertase (14.3 U), glucose oxidase (10,000 U) and catalase (59,000 U).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sucrose/conversion; Fructose; Gluconic acid; Invertase; Glucose oxidase; Catalase.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200022
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Levans production by Gluconacetobacter diazotrophicus Electron. J. Biotechnol.
Molinari,María Laura; Boiardi,José Luis.
Background: Growth of Gluconacetobacter diazotrophicus with glucose as carbon an energy source has been extensively studied. However, there are no reports in the literature describing growth of G. diazotrophicus in cultures containing sucrose as carbon source. The first step in sucrose pathway and production of levans was investigated. Biomass, levans, gluconic acid and keto gluconic acids production and levansucrase activity were determined in cultures with different sucrose concentration and nitrogen sources. Results: The biomass production was maximal in cultures containing 100 g x L-1 sucrose and inorganic nitrogen. Gluconic acid production was observed under all conditions tested, at levels up to 9 g x L-1 in cultures with sucrose excess and...
Tipo: Journal article Palavras-chave: Gluconic acid; Keto gluconic acids; Levans; Levansucrase; Sucrose metabolism.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300007
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