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	Provedor de dados BJMBR (1) Braz. J. Plant Physiol. (1) Autor Almeida-de-Faria,M. (1) Alves,M.J.M. (1) Colli,W. (1) Miletti,L.C. (1) Mishra,Sanjay (1) Sangwan,R.S. (1) Singh,I.V. (1) Tyagi,Abhishek (1) Mais... Palavra-chave Glycolipids (2) Catharanthus roseus (1) Fucose (1) Fucosidase (1) Glycoproteins (1) Leaf development (1) Phospholipids (1) Senescence (1) Sterol (1) Trypanosoma cruzi (1) Trypanosomatids (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2006 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Immunocytochemical and biochemical detection of alpha-L-fucosidase in Trypanosoma cruzi BJMBR Miletti,L.C.; Almeida-de-Faria,M.; Colli,W.; Alves,M.J.M.. The aim of the present study was to demonstrate the presence of alpha-L-fucosidase in Trypanosoma cruzi. Immunocytochemical and biochemical techniques were used to localize and characterize a membrane-associated, neutral-pH-optimum, alpha-L-fucosidase from the parasite. Light and electron microscopy localized the alpha-L-fucosidase specifically on the surface of the parasite and on membranes in the posterior region of the epimastigote stage. Although much less intense, labeling was also detected on the surface of trypomastigotes. At least 50% of the alpha-L-fucosidase activity was associated with epimastigote membrane solubilized with 1 M NaCl or 1% Triton X-100, suggesting that alpha-L-fucosidase is peripherally associated with membranes. The enzyme from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosoma cruzi; Trypanosomatids; Fucosidase; Fucose; Glycoproteins; Glycolipids. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500006 Changes in lipid profile during growth and senescence of Catharanthus roseus leaf Braz. J. Plant Physiol. Mishra,Sanjay; Tyagi,Abhishek; Singh,I.V.; Sangwan,R.S.. Various lipid classes and compounds were monitored during the period of leaf emergence to leaf drop of Catharanthus roseus. The expansion to early maturation phase was accompanied by cellular build-up of all major lipid classes, whilst aging and senescence were characterized by their substantial decline, except for the neutral lipids; the leaf monogalactosyl diglyceride/digalactosyl diglyceride ratio decreased from 4.3 (complete maturity) to 2.1 (abscised stage). The early maturation stage was the earliest stage when appreciable amounts of free sterols and fatty acids could be observed. Sterol/phospholipids ratios increased by 68-fold in the abscised leaf as compared to that at full maturity. The unsaturated/saturated fatty acid ratio was far lower in the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Catharanthus roseus; Glycolipids; Leaf development; Phospholipids; Senescence; Sterol. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000400002 Registros recuperados: 2 Primeira ... 1 ... Última

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