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| Registros recuperados: 111 | |
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| Freitas,Vicente J.F.; Serova,Irina A.; Andreeva,Lyudmila E.; Dvoryanchikov,Guennadi A.; Lopes-Jr.,Edilson S.; Teixeira,Dárcio I.A.; Dias,Luciene P.B.; Avelar,Suely R.G.; Moura,Raylene R.; Melo,Luciana M.; Pereira,Alexsandra F.; Cajazeiras,João B.; Andrade,Maria L.L.; Almeida,Karlliely C.; Sousa,Francisco C.; Carvalho,Antonio C.C.; Serov,Oleg L.. |
| In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats were used as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulated by a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal. Ovulation was induced by injecting 100µg GnRH 36 h after sponge removal. The recipients also received an estrus synchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal, zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliable visualization of the pronuclei. The DNA construct containing hG-CSF... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transgenesis; Goat; HG-CSF; DNA microinjection; Embryo. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000400003 |
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| Cordeiro,M.F.; Teixeira,P.P.M.; Oliveira,M.E.F.; Filippo,P.A. Di; Dias,D.P.M.; Beretta,C.A.G.; Dória,R.G.S.; Feliciano,M.A.R.; Coutinho,L.N.; Vicente,W.R.R.. |
| The objective of this study was to assess the reproductive response of adult and prepubertal goats subjected to repeated laparoscopic ovum pick-up (LOPU). The study animals were divided into two groups, specifically, adult nanny goats (GA, n=10) and prepubertal nanny goats (GP, n=10), which were subjected to estrous synchronization and ovarian stimulation for LOPU. Both groups underwent six LOPU procedures at seven-day intervals and were subsequently subjected to controlled mating and pregnancy diagnosis to evaluate their future fertility. The study showed a reduction in the number of follicles visualized and in the amount and quality of the oocytes that were recovered and exposed to in vitro maturation. As indicated by the fertility test, however, no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Laparoscopy; Follicular puncture; Oocyte recovery. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100020 |
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| Rodrigues,G.Q.; Lima,I.M.T.; Chaves,R.N.; Rossetto,R.; Costa,S.L.; Castro,S.V.; Barros,V.R.P.; Matos,M.H.T.; Lopes,C.A.P.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.. |
| The objectives of this study were to investigate whether TGF-β affect the survival, activation and further growth of goat primordial follicles enclosed in ovarian cortex after in vitro culture. Goat ovaries were collected from an abattoir and pieces of ovarian tissues were cultured for one or seven days in a supplemented alpha Minimum Essential Medium, alone or containing TGF-β (1, 5, 10 or 50ng/mL). Ovarian tissues from the fresh control as well as those cultured were processed for histological and ultrastructural studies. The results showed that when compared with fresh control, there was decrease in the percentages of histologically normal follicles in all treatments only after seven days culture. TGF-β did not affect the activation of preantral... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preantral follicle; In vitro development; TGF-β. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200013 |
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| Silva,J.S.; Castro,R.S.; Melo,C.B.; Feijó,F.M.C.. |
| The prevalence of caprine arthritis-encephalitis (CAE) infection in dairy goat herds from Rio Grande do Norte State, Brazil, was estimated, and the predisposing factors to the infection were identified. An epidemiologic survey was held to collect information on educational level of the owner and origin of the animals, and on sanitation and vaccination practices, diseases, morbidity and mortality rates in the herds. The prevalence of CAE virus infection was 11%, and CAE was always observed in some goats in all studied municipalities. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; CAEV; Prevalence; Arthritis; Encephalitis; Predisposing factor. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600003 |
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| Nóbrega Jr.,J.E.; Rossetto,R.; Matos,M.H.T.; Chaves,R.N.; Magalhães,D.M.; Lima-Verde,I.B.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.; Gonçalves,P.B.D.; Oliveira,J.F.C.. |
| This study describes the effect of sphingosine 1-phosphate (S1P) for development of preantral follicle, therefore the activation and follicular viability of caprine follicles cultured in vitro. Ovarian fragments were cultured for 1 or 7 days in Minimum Essential Medium with different S1P concentrations (0, 1, 10, 50, 100 or 200ng/mL). All ovarian fragments were processed for histological analysis in optical microscopy, transmission electron microscopy and fluorescence analysis. The treatment using 1ng/mL of S1P was able to maintain the percentage of normal follicles with the progression of the culture from day 1 to 7. At end of the 7-day culture period there was a significant reduction (P<0.05) in the percentage of primordial follicles in all groups... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preservation; Preantral follicles; Sphingosine 1-phosphate; Follicular activation. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000400977 |
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| Bandeira,D.A.; Castro,R.S.; Azevedo,E.O.; Nascimento,E.R.; Melo,L.S.S.; Melo,C.B.. |
| O objetivo deste trabalho foi verificar a ocorrência de Mycoplasma agalactiae, com o uso da reação em cadeia da polimerase (PCR), em 30 rebanhos caprinos leiteiros nas microrregiões do Cariri Ocidental e Oriental do estado da Paraíba. Das 120 amostras de leite testadas, nove (7,5%) apresentaram amplificação de apenas um fragmento com 360pb. As amostras que amplificaram este fragmento estavam presentes em 6/30 (20%) fazendas localizadas nos municípios de Amparo, Santo André e Gurjão. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Goat; Mycoplasmosis; Milk; CASG. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000500031 |
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| Ribeiro,M.G.; Lara,G.H.B.; Bicudo,S.D.; Souza,A.V.G.; Salerno,T.; Siqueira,A.K.; Geraldo,J.S.. |
| Relata-se, pela primeira vez no Brasil, a ocorrência de mastite gangrenosa caprina atípica causada pela co-infecção por Staphylococcus aureus, Clostridium perfringens e Escherichia coli em uma cabra da raça Boer, na segunda semana de lactação. Descrevem-se os achados clínicos, os procedimentos de diagnóstico microbiológico e a conduta terapêutica. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Gangrenous mastitis; Etiology; Co-infection. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300037 |
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| Rule,R.; Lacchini,R.; Mordujovich,P.; Antonini,A.. |
| Avaliaram-se as variáveis cinéticas da cefepime e a produção de leite de cabras após a administração parenteral (intravenosa e intramuscular) de cefepime, com e sem reação inflamatória na região implantada. Dez cabras em lactação, implantadas com caixas de material sem reação imunológica para colher o fluido de tecido (FT) foram usadas em dois experimentos. No primeiro a aplicação de cefepime foi feita na primeira semana após a implantação e no segundo na oitava semana. Na primeira semana após a implantação observou-se elevação dos níveis de proteína no fluido do tecido após uma simples dose de 20mg/kg de cefepime via endovenosa ou intramuscular. Amostras do sangue e do leite foram obtidas e as variáveis cinéticas foram avaliadas. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cefepime; Goat; Kinetic; Milk; Production. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000100018 |
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| Fonseca,J.F.; Esteves,L.V.; Zambrini,F.N.; Brandão,F.Z.; Peixoto,M.G.C.D.; Verneque,R.S.; Siqueira,L.G.B.; Viana,J.H.M.. |
| O objetivo do presente estudo foi avaliar a viabilidade da técnica de transferência não cirúrgica em cabras. Quatro cabras não-lactantes pluríparas da raça Toggenburg foram utilizadas como receptoras de embriões, sendo que duas receberam um embriões e duas receberam dois embriões coletados não cirurgicamente cabras doadoras. Os corpos lúteos das receptoras foram detectados um dia antes da transferência de embriões por ultrassonografia transretal. Uma seringa de 5mL contendo 2mL de meio holding foi acoplada em um cateter tomcat, no qual os embriões foram aspirados em uma coluna central a duas outras colunas. Um espéculo Colin número 2 foi inserido na vulva e na vagina, e com o uso de uma fonte de luz, a cerviz foi localizada e imobilizada com uma pinça de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Non-surgical embryo transfer; Cervical via. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200040 |
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| Silva Júnior,L.C.; Arruda,L.C.P.; Silva,D.G.B.; Soares,F.A.P.; Borba Neto,A.V.; Leite,J.E.B.; Soares,P.C.. |
| A seven month old female goat showed neck swelling, apathy, appetite and weight loss, restlessness, increased salivation, cough, and regurgitation episodes which occurred post feeding. The animal was evaluated through both clinical and radiographic examinations. Plain radiography was performed and contrasted X-ray was done using barium sulfate. For radiological examination, the lateral projection was used for analysis. The results showed a radiopaque content in the esophageal lumen dorsally positioned to heart with density and appearance similar to the rumen. A dilation of the esophagus was reported and a diagnosis of megaesophagus was made. In conclusion, contrast esophagography must be included in the diagnosis of megaesophagus in goats. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Goat; Esophagus; Radiography; Diagnosis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300033 |
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| Fonseca,J.F.; Maffili,V.V.; Santos,A.D.F.; Fürst,R.; Prosperi,C.P.; Rovay,H.; Souza,J.M.G.; Torres,C.A.A.. |
| This study reported the effects of prostaglandin (PGF2a) administration 10 days apart on reproductive parameters of cyclic artificial inseminated (AI) nulliparous Alpine (n=9) and Saanen (n=9) goats. Animals received two doses of 22.5mg PGF2a 10 days apart. After 1st and 2nd PGF2a administrations, estrus was monitored at 12 h intervals, with a buck teaser. Plasma progesterone concentration (ng/mL) was determined from blood sampled on day 0 (1st PGF2a) and the following 5, 10 (2nd PGF2a), 15, 20, 25 and 30 days. After the onset of the second estrus, females were transrectally (5 MHz probe) scanned at 4 hour intervals until at least 8h after ovulation. Pregnancy was checked through transrectal ultrasound on days 20, 25, 30, 35 and 90 after insemination. All... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Estrus; Ovulation; Progesterone; Embryo loss; Goat. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200014 |
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| Ramírez-Bribiesca,J.E.; Tórtora,J.L.; Huerta,M.; Hernández,L.M.; López,R.; Crosby,M.M.. |
| Three experiments were carried out to determine the optimum selenium-vitamin E injection level to maintain acceptable blood selenium (Se) status of does and kids, as well as to determine the relation of that status to mortality rates in kids. In experiment 1, 238 goats were assigned to one of three groups during the mating period: A1-control, A2- 0.06mgSe+0.8IU vitE/kgBW and A3-0.125mgSe+1.7IU vitE/kgBW. No differences (P>.05) for fertility and prolificacy were observed among the groups. Blood Se concentration did not differ among Se-vit E groups and control group before injection, and both groups showed Se deficient condition. There was a trend (P<.05, 32%) to increase Se blood level 60 days post-treatment, but difference was not observed between A2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Selenium; Vitamin E; México. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000100011 |
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| Fonseca,J.F.; Batista,R.I.T.P.; Souza-Fabjan,J.M.G.; Oliveira,M.E.F.; Brandão,F.Z.; Viana,J.H.M.. |
| ABSTRACT The efficiency of an alternative freezing protocol for goat embryos of different morphology and quality was tested. Fifty-eight embryos on Day 6-7 stage were transferred as fresh or after freeze-thawing (n=29/group). For freezing, embryos were placed into 1.5M ethylene-glycol solution for 10min. During this time, they were loaded in the central part of 0.25mL straw, separated by air bubble from columns containing PBS/BSA 0.4% plus 20% BFS. Straws were then frozen using a freezing machine from 20ºC to -6ºC at a cooling rate of 3ºC/min, stabilization for 15min (seeding after 5min), from -6 C to -32ºC at 0.6 C/min,and plunged into liquid nitrogen. Frozen embryos were thawed for 30s at 37ºC in a water bath. Embryos subjected to fresh transfer were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo survival; Embryo transfer; Goat; Slow freezing. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501489 |
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| Wang,Y.; Qin,X.; Chen,Y.; Xing,J.; Gao,J.; Qiu,D.. |
| RESUMO O objetivo do presente estudo foi investigar a soroprevalência de infecção com Toxoplasma gondii (T. gondii) em cabras em cinco províncias do noroeste da China. Soroprevalência foi determinado usando o kit de teste de ensaio de imunoabsorção enzimática (ELISA). A soroprevalência geral foi 21.23% (197/928). Análise estatística revlou que diferenças significativas foram observadas em fêmeas (P= 0.048, OR= 0.567, 95% CI= 0.309 a 1.041) e nos grupos ≥ 2 (P= 0.002, OR= 0.330, 95% CI= 0.224 a 0.488). Nenhuma diferença estatisticamente significativa foi observada entre diferentes províncias. Nossos resultados indicam que a infecção com T. gondii, que pode ter implicações importantes sobre a saúde pública, teve diferenças significativas em sexo e idade, mas... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Toxoplasma gondii; Seroprevalence; Goat; Five northwestern provinces. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100277 |
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| Magalhães,D.M.; Araújo,V.R.; Lima-Verde,I.B.; Matos,M.H.T.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.. |
| Porcine pituitary follicle stimulating hormone (pFSH) is known to regulate the production of growth factors that have an essential role in early foliculogenesis. However, the effects of different preparations of pFSH on the survival and development of caprine follicles are not yet known. The aim of this study was to evaluate the effects of different pFSH (Stimufol® and Folltropin®) on the in vitro survival and growth of caprine preantral follicles. Pieces of caprine ovarian tissues were cultured for either one or seven days in a supplemented Minimum Essential Medium, alone or containing either Stimufol® (50 ng/mL) or Folltropin® (10, 50, 100 and 1000 ng/mL). Fresh control ovarian tissues as well as cultured tissued were processed for histological and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preantral follicles; FSH; Follicular growth. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200003 |
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| Moura,Raylene Ramos; Melo,Luciana Magalhães; Freitas,Vicente José de Figueirêdo. |
| Among all the transgenic mammalians produced so far, goats have represented an excellent model of transgenesis when considering the factors such as the market demand for protein, volume of milk produced per lactation and reproductive rate. Various recombinant proteins have been obtained from the transgenic and non-transgenic goats, and among these, human antithrombin, produced by the transgenic goats, was the first recombinant protein of animal origin to be released as a drug for the clinical use in humans. This review reports the aspects inherent to the production of recombinant proteins in the goats, from the production of the animal bioreactors up to the expression of these proteins in their milk. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transgenesis; Goat; Recombinant protein; Milk. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500010 |
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| SANTOS,Renato de Lima; BARRETO FILHO,João Bosco; MARQUES Jr.,Antônio de Pinho; ANDRADE,Joelmer Santos. |
| The goal of this research was to determine the volumetric proportions of the structural components of the goat placenta throughout gestation. We used nine goats of nondefined breed, separated into groups A, B and C, with 90, 120 and 150 days of gestation, respectively. From each animal fragments of three placentomes were obtained, one removed from the base, another from the middle part and the other from the end of the gestational uterine horn. The fragments were fixed in Bouin solution and histologically processed according to the standard techniques and stained with hematoxilin-eosin. Microscopically, a 25-point circular grid (Zeiss KPL ocular 6.3x with 25 points) was randomly used across the placentome to determine the volumetric proportion of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Placenta; Morphology; Goat. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000400002 |
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| Registros recuperados: 111 | |
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