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Callus induction and plant regeneration of Ulex europaeus Electron. J. Biotechnol.
Ramírez,Ingrid; Dorta,Fernando; Cuadros-Inostroza,Álvaro; Peña-Cortés,Hugo.
A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets...
Tipo: Journal article Palavras-chave: Antioxidants; Callus induction; Fabaceae; Gorse; Plant regeneration; Ulex europaeus.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400007
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