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Optimal culture conditions for the initial development of Ilex paraguariensis A.St.-Hil. explants Acta Botanica
Griebeler,Ana Gláucia; Consatti,Guilherme; Freitas,Elisete Maria de; Sperotto,Raul Antonio.
In the state of Rio Grande do Sul, Brazil, yerba mate (Ilex paraguariensis A. St.-Hil.) is an economically and culturally important species. Because of the difficulty in obtaining plantlets from seeds, micropropagation has emerged as an alternative that could increase production. This work aimed to evaluate the effect that treatment with 6-benzyladenine (BA), at concentrations of 2.2, 8.9, and 20 µM and associated with naphthaleneacetic acid (NAA, 0.5 µM), has on the in vitro growth and leaf emission of I. paraguariensis nodal segments cultivated in 0.5 X Murashige and Skoog (MS) medium or 0.5 X Woody Plant Medium (WPM). The most efficient treatment was MS medium with 8.9 µM BA+NAA, in which I. paraguariensis presented greater shoot elongation and higher...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquifoliaceae; Growth regulators; Naphthaleneacetic acid; Vegetative micropropagation; Yerba mate.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000400008
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Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses Anais da ABC (AABC)
PáDUA,MARLÚCIA S.; PAIVA,LUCIANO V.; LABORY,CLAÚDIA R.G.; ALVES,EDUARDO; STEIN,VANESSA C..
ABSTRACTOil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses inE.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryogenic potential; Growth regulators; Tissue culture; Transmission electron microscopy.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401545
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Effect of growth regulators application on the quality maintenance of 'Brookfield' apples Bragantia
Brackmann,Auri; Thewes,Fabio Rodrigo; Santos,Luana Ferreira dos; Machado,Eduardo Perkovski; Ludwig,Vagner; Artmann,Líniker da Silva.
AbstractThe main goal of the present study was to elucidate the effect of growth regulators at harvest and postharvest quality of 'Brookfield' apples stored under controlled atmosphere through a multivariate approach. Thus, an experiment with two steps (field and storage) was carried out. The treatments in field were applied with an output of 1,000 L ha–1 of water. The following treatments were tested: Control: only water application; AVG (aminoethoxyvinylglycine): 0.83 kg ha–1 of Retain® applied 30 days before harvest (BH); NAA (naphthalene acetic acid): 40g ha–1of naphthalene acetic acid applied 7 days BH; Ethephon: 2.0 L ha–1 of Ethrel® applied 10 days BH; 1-MCP: 0.625µL L–1 of 1-MCP (1-methylcyclopropene): applied during postharvest (storage); LE (low...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Apple; Growth regulators; Quality maintenance; Storage.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000400453
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Micropropagation of Salvia guaranitica Benth. through axillary shoot proliferation BABT
Echeverrigaray,Sergio; Carrer,Rosane Postingher; Andrade,Luciana Bavaresco.
Nodal segments from greenhouse grown adult plants of Salvia guaranitica were used to evaluate the effect of culture media and growth regulators on the micropropagation and growth. The highest multiplication rate was obtained on Murashige and Skoog (MS) medium supplemented with 2.22 µM of 6-benzylaminopurine (BAP). The best condition for rooting was MS medium with 2.85 µM indole-3-acetic acid (IAA). Rooted plants were successfully acclimatized and exhibited a normal development until maturity. Using the described protocol, approximately 35 plants per explant were obtained after three months.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth regulators; Shoot culture; Axillary buds.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400018
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Organogenesis from in vitro-derived leaf and internode explants of Hoya wightii ssp. palniensis -a vulnerable species of Western Ghats BABT
Lakshmi,Subbaiah Revathi; Benjamin,Jambarapu Herald Franklin; Kumar,Tirupathi Senthil; Murthy,Garimella Venkata Suryanarayana; Rao,Mandali Venkateswara.
An efficient system was developed for indirect plant regeneration from in vitro-derived leaf and internode explants of Hoya wightii ssp. palniensis. Maximum percentage of the organogenic callus was obtained on MS medium supplemented with NAA (1.0 mg/l) and 2,4-D (2.0 mg/l). The best shoot bud induction was observed on MS medium with BA (1.0 mg/l) +IBA (0.5 mg/l). The coconut water (15%) was better, resulting in a differentiation of the shoot initials in to well-developed shoots. The elongated shoots (› 3cm long) were rooted on a full strength MS basal medium, supplemented with 0.2 mg/l of IBA. Finally, the rooted plants were transferred to the soil with 80% success rate. This protocol was utilized for the in vitro propagation of this endangered plant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro explants; Organogenesis; Hoya wightii ssp. Palniensis; Vulnerable plant; Growth regulators.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300010
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Indole-3-acetic acid metabolism in normal and dwarf micropropagated banana plants (Musa spp. AAA) Braz. J. Plant Physiol.
Zaffari,Gilmar Roberto; Peres,Lázaro Eustáquio Pereira; Tcacenco,Fernando Adami; Kerbauy,Gilberto Barbante.
Nanism is one of the most frequent type of mutant in micropropagated banana plants from the Cavendish subgroup. The present study aimed at studying some of the hormone factors involved in this type of mutation. Rhizomes from normal and dwarf plants from the cultivar Grand Naine were incubated for 5 d in the presence of [³H]-L-tryptophan, [³H]-indole-3-acetic acid and gibberellin, to quantify the endogenous levels of indole-3-acetic acid-ester, indole-3-acetic acid-amide, free indole-3-acetic acid, and cytokinins. The endogenous levels of indole-3-acetic acid and its ester- and amide-conjugated forms were measured in normal and dwarf plants incubated for 30 d with gibberellin, indole-3-acetic acid, and L- and D-tryptophan. In normal plants, the use of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Indole-3-acetic acid metabolism; Growth regulators; Somaclonal variation.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000300005
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Flowering and vegetative growth of olive tree submitted to pruning and paclobutrazol application Braz. J. Plant Physiol.
Cruz,Maria C. M.; Oliveira,Adelson F.; Oliveira,Dili L.; V. Neto,João.
This work focuses on the evaluation of flowering and vegetative growth of olive tree submitted to pruning and paclobutrazol application under field conditions with low temperatures during the winter. Two-years-old olive plant variety Grappolo 575, were submitted to light pruning, removing apical dominance, before treatments application. The treatments were organized in a 4×2 factorial scheme, respective to four PBZ concentrations tested: 0, 200, 400 and 800 mg L-1 of PBZ and two plants groups, with and without pruning, in randomized block with four replications. Paclobutrazol concentrations tested did not affect the olive tree flowering. The plant vegetative growth was reduced until 60 days after paclobutrazol application. Pruning resulted in stimulation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Floral induction; Growth regulators; Management; Olea europaea L.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200002
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Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L. Ciência e Agrotecnologia
Oliveira,Lenaldo Muniz de; Paiva,Renato; Santana,José Raniere Ferreira de; Pereira,Flávia Dionísio; Nogueira,Raírys Cravo; Silva,Luciano Coutinho.
Annona glabra is a tropical species that has significant agronomic potential in terms of furnishing fruits for in natura consumption and for the production of phyto-pharmaceuticals. In vitro cultivation has been considered the most promising form of propagation for this species, although large scale utilization of this technique is currently limited by high rates of leaf abscission, reduced rates of explant multiplication and slow bud growth. The present work evaluated the effects of different cytokinins on mineral accumulation in shoots of A. glabra cultivated in vitro, and their effects on growth and survival of these plants. Buds of A. glabra were cultivated in Wood Plant Medium (WPM) in the presence of 6-benzilaminopurine (BAP), thidiazuron (TDZ),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth regulators; Annonaceae; Mineral nutrition.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000600012
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Tissue culture of Cecropia glaziovii Sneth (urticaceae): vegetative micropropagation and plant regeneration from callus Ciência e Agrotecnologia
Alves,Marcos Nopper.
Cecropia glaziovii is a tree with used in Brazilian popular medicine. Methods allowing the clonal propagation of this species are of great interest for superior genotype multiplication and perpetuation. For this reason, we examined the effect of different culture media and different types of explants on adventitious shoot regeneration from callus and buds of C. glaziovii. Leaves, petioles and stipules obtained from aseptically grown seedlings or from pre-sterilized plants were used to initiate cultures. Adventitious shoot regeneration was achieved when apical and axillary buds were inoculated on gelled Murashige & Skoog (MS) medium supplemented with 6-benzylaminopurine alone (BAP) (1.0, 5.0 or 10.0 mg L-1) or combined with -naphthalene acetic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Clonal propagation; Adventitious buds; Growth regulators; Callogenesis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500024
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Characteristics of banana fruits (Musa spp. AAA, cv. Nanica) treated with cytokinin and gibberellin Ciencia e Investigación Agraria
Lima,Juliana D; Santa Rosa,Jéssica; Gomes,Eduardo N; Rozane,Danilo E; Modenese-Gorla da Silva,Silvia H.
The aim of this study was to analyze the effect of cytokinin and gibberellin on the characteristics of banana fruits (Musa spp. AAA, cv. Nanica) as a function of the formation period and position in the bunch. A completely randomized, 2x5 factorial design was adopted consisting of two bunch development periods (summer and winter) and five treatments as well as the same arrangement in a split plot with the 2×5 factorial arrangement as the plot and the position of the hand in the bunch (basal, middle and apical) as the subplot. Treatments consisted of spraying twice with either water, 150 mg L-1 of kinetin, 200 mg L-1 of gibberellic acid, 100 mg L-1 of kinetin plus 200 mg L-1 of gibberellic acid or 200 mgL-1 of kinetin plus 200 mg L-1 of gibberellic acid,...
Tipo: Journal article Palavras-chave: Growth regulators; Intra-bunch variability; Source-sink relationship.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000200005
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Adaptation of in vitro regeneration protocol for Brazilian wheat genotypes Ciência Rural
Roesler,Eduardo André; Manfroi,Ernandes; Morás,Andréa; Didoné,Dielli Aparecida; Grando,Magali Ferrari; Lau,Elene Yamazaki.
ABSTRACT: The availability of an efficient protocol for in vitro regeneration is imperative for genetic transformation. Using genotypes adapted to the target region as a transgenic platform accelerates the development of cultivars. Therefore, this study aimed to adapt an in vitro regeneration protocol for Brazilian wheat genotypes. For this purpose, the in vitro regeneration capacity of immature embryos from six Brazilian wheat genotypes using two protocols of regeneration of somatic embryos was analysed. Furthermore, combinations of 2,4-D and picloram in the callus induction medium were tested in order to improve regeneration efficiency. Genotypes with higher regeneration efficiency were BR18-Terena and PF020037, yielding 0.42 and 1.13 shoots per explant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Tissue culture; Growth regulators.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100402
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Callus induction and plant regeneration of tomato (Lycopersicon esculentum cv. IPA 5) via anther culture Ciência Rural
Brasileiro,Ana Christina Rabello; Willadino,Lilia; Carvalheira,Gianna Griz; Guerra,Marcelo.
Different growth regulators combinations were tested on the production of anther callus in tomato cultivar IPA 5. Calli were induced on media supplemented with 1.0mgL-1 gibberellic acid (GA3), 0.05mgL-1 alpha-naphthaleneacetic acid (NAA) plus 0.1mgL-1 6-benzylaminopurine (BAP), or with 1.0mgL-1 BAP plus 1.0mgL-1 NAA. The medium containing 1.0mgL-1 BAP and 1.0mgL-1 NAA produced the highest calli frequency, and promoted plant regeneration by indirect organogenesis, when calli were transferred to 0.01mgL-1 BAP and 0.001mgL-1 NAA. Plants regenerated presented tetraploid cells and rare diploid cells. These tetraploid plants could be used as source for further obtainment of trisomic lines, for the purpose of genic localization studies and protein compounds...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth regulators; Organogenesis; Tetraploid cells.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000400008
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Calli induction in leaf explants of coffee elite genotypes Ciência Rural
Rezende,Juliana Costa de; Carvalho,Carlos Henrique Siqueira de; Pasqual,Moacir; Santos,Ana Carolina Ramia; Carvalho,Stephan Malfitano de.
Three experiments were carried out with the objective of achieving high effectiveness in calli induction from high heterozygosis leaf explants of Coffea arabica through indirect somatic embryogenesis. A randomized-block design in a 2x5 factorial arrangement made up of two media [BOXTEL & BERTHOULY (1996) and TEIXEIRA et al. (2004)] and five C. arabica genotypes were used in the first experiment. In the second experiment the embryogenic calli production potential was evaluated in ten genotypes. Each of them was considered as a treatment. In the third experiment the variations in both 2.4-D (2.5 e 20µM) and 2-iP (2.5 e 20µM) concentrations in TEIXEIRA et al. (2004) medium and secondary media were evaluated. Crops were kept in a growth room under...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea arabica; Culture medium; Growth regulators.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000300004
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Interacciones causales del necrosamiento de yemas florales en zarzamora 'Tupy'. Colegio de Postgraduados
Argote Hernández, Cajeme Nicolás.
Actualmente en México la principal frutilla que se exporta en fresco es la zarzamora ‘Tupy’ (Rubus spp.). El Estado de Michoacán produce más de 90% del total a nivel nacional. Uno de los problemas de producción es el necrosamiento de las yemas florales. No existen reportes documentados sobre el necrosamiento de las yemas en zarzamora. Se diseñó un experimento con zarzamora ´Tupy´ en Los Reyes, Michoacán para diagnosticar las posibles interacciones causales del necrosamiento de yemas. Se probaron cinco fechas de forzado (defoliación), tres tratamientos de dosis y formas variables de aplicación de thidiazurón (TDZ) y ácido giberélico (AG3) en pre y post defoliación y dos dosis de aplicación edáfica de nitrógeno (350 a 500 unidades/ha.). La primera fecha...
Palavras-chave: Rubus; Frutillas; Reguladores de crecimiento; Rendimiento; Fruticultura; Maestría; Small fruits; Growth regulators; Yield.
Ano: 2012 URL: http://hdl.handle.net/10521/1788
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Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture Electron. J. Biotechnol.
Parida,Reena; Mohanty,Sujata; Kuanar,Ananya; Nayak,Sanghamitra.
An efficient protocol has been established for rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga L, a rare medicinal plant. Different plant growth regulators like Benzyladenine (BA), Indoleacetic acid (IAA), Indolebutyric acid (IBA), Napthaleneacetic acid (NAA) and adenine sulphates (Ads) have been tried for induction of multiple shoots using lateral bud of rhizome as explants. The highest rate of shoot multiplication (11.5 ± 0.6) shoot/explant as well as leaf biomass production (7.4 ± 0.3) gram/explant was observed on Murashige and Skoog medium supplemented with Benzyladenine (1 mg/l) and Indoleacetic acid (0.5 mg/l). Data of shoot multiplication and leaf biomass production were statistically analysed. Upon excission of...
Tipo: Journal article Palavras-chave: Growth regulators; Kaempferia; In vitro propagation; Leaf biomass.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400005
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Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers Electron. J. Biotechnol.
Lakshmanan,Venkatachalam; Reddampalli Venkataramareddy,Sreedhar; Neelwarne,Bhagyalakshmi.
A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cultures with distinct variation in morphological characteristics (morphotypes). Similarly, the mother maintained in the field was also subjected for genetic analysis. Out of the 50 RAPD and 25 ISSR primers screened, 30 RAPD and 5 ISSR primers produced totally 424 clear, distinct and reproducible band classes resulting in a total of 5088 bands where the banding patterns...
Tipo: Journal article Palavras-chave: Growth regulators; Musa; Protocol; Shoot cultures; Somaclonal variation.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100010
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Effect of salicylic acid on pre-transplant acclimatization of native tomato populations Horticultura Brasileira
Poot-Poot,Wilberth A; Delgado-Martínez,Rafael; Castro-Nava,Sergio; Segura-Martínez,Ma Teresa; Carreón-Pérez,Alejandro; Hernández-Martínez,Jesús Guillermo.
ABSTRACT The aim of this study was to evaluate the effect of salicylic acid (SA) application on pre-transplant acclimatization of native populations of tomato. SA is a growth regulator that modifies plant growth and development by inducing changes in cell processes, physiology and morphology. Five populations of native tomato were sown in polystyrene trays. Peat moss was used as substrate and plants were maintained at field capacity continuously. After emergence, seedlings were applied during three weeks with different concentration of SA (0.0, 0.01, 0.1, 0.5 and 1.0 μM). A completely random experimental design was used with five replications per treatment. The growth parameters evaluated were height, stem diameter, number of leaves, hypocotyl length,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; Stress tolerance; Chlorophyll; Carotenoids; Growth regulators.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400480
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Development and production of sweet corn applied with biostimulant as seed treatment Horticultura Brasileira
Lima,Sebastião F; Jesus,Anderson A; Vendruscolo,Eduardo P; Oliveira,Tabata Raissa; Andrade,Maria Gabriela O; Simon,Cátia Aparecida.
ABSTRACT Sweet corn has potential to increase farmer’s income. Thus, biostimulants may contribute to increase productivity of sweet corn, being indicated and used in various crops. The objective of this study was to evaluate the effects of applying biostimulant on sweet corn crops and to establish the ideal dose aiming to increase the crop’s agronomic characteristics. The experiment was installed in the municipality of Chapadão do Sul-MS and was conducted from November 15, 2012 to February 19, 2013. Treatments were composed of eight biostimulant doses (0.0, 4.0, 8.0, 12.0, 16.0, 20.0, 24.0 and 28.0 mL kg-1 of seeds), arranged in a randomized complete block design with four replicates. The responses of the variables to the best dose of biostimulant were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays convar. saccharata var. rugosa (sweet corn); Phytohormones; Growth regulators.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000100094
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Micropropagation of the ornamental vulnerable bromeliads Aechmea blanchetiana and Aechmea distichantha Horticultura Brasileira
Santa-Rosa,Sandra; Souza,Fernanda VD; Vidal,Ádila M; Ledo,Carlos Alberto da S; Santana,José RF de.
Aechmea blanchetiana and Aechmea distichantha are Brazilian native bromeliads with great ornamental value, although none of the species are produced commercially. Therefore, market demands are met through nature predatory exploitation. This study intended to (1) evaluate the in vitro seed germination of A. blanchetiana and A. distichantha and (2) establish micropropagation protocols for both species, aiming at producing plantlets for the market, as well as to germplasm in vitro conservation. Germination experiments were carried out in completely randomized designs with two treatments (presence and absence of light) and seven and eight replications respectively for A. blanchetiana and A. distichantha. In vitro seedlings, produced under light conditions,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromeliaceae; Tissue culture; In vitro multiplication rate; Growth regulators.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000100018
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Produção in vitro de mudas de Coroa-de-Frade (Melocatus oreas Miq. - Cactaceae): uma espécie nativa da Caatinga de potencial ornamental. Infoteca-e
SOUZA, A. V. de; SOUZA, D. D. de; SILVA, N. B. G. da; OLIVEIRA, F. J. V. de.
Objetivou-se com este trabalho iniciar os estudos para a obtenção de plantas in vitro, a fim de otimizar, posteriormente, um protocolo para a produção de mudas em escala comercial.
Tipo: Folhetos Palavras-chave: Coroa-de-frade; Melocatus oreas; Bioma; Bioma Caatinga; Propagação de plantas; Caatinga; Cactaceae; Espécie Nativa; Muda; Planta Ornamental; Regulador de Crescimento; Micropropagação; Growth regulators; Micropropagation; Ornamental plants.
Ano: 2012 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/930459
Registros recuperados: 39
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