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Keller,R.; Zago,A.; Viana,M.C.; Bourboulia,D.; Desgranges,C.; Casseb,J.; Moura,W.V.; Dietze,R.; Collandre,H.. |
The aims of the present study were to determine the prevalence of human herpesvirus type 8 (HHV-8) in HIV-positive Brazilian patients with (HIV+/KS+) and without Kaposi's sarcoma (HIV+/KS-) using PCR and immunofluorescence assays, to assess its association with KS disease, to evaluate the performance of these tests in detecting HHV-8 infection, and to investigate the association between anti-HHV-8 antibody titers, CD4 counts and staging of KS disease. Blood samples from 66 patients, 39 HIV+/KS+ and 27 HIV+/KS-, were analyzed for HHV-8 viremia in peripheral blood mononuclear cells by PCR and HHV-8 antigenemia for latent and lytic infection by immunofluorescence assay. Positive samples for latent nuclear HHV-8 antigen (LNA) antibodies were titrated out from... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AIDS-related Kaposi's sarcoma; Human herpesvirus type 8; HHV-8; Brazil. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700007 |
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Souza,V.A.U.F.; Sumita,L.M.; Freire,W.; Sato,H.K.; Grandi,J.L.; Pierrotti,L.C.; Nascimento,M.C.; Pannuti,C.S.. |
Human herpesvirus 8 (HHV-8) is a newly described herpesvirus that is etiologically associated with all forms of Kaposi's sarcoma (KS). Seroepidemiological studies have shown high prevalence rates of HHV-8 antibodies among men who have sex with men (MSM) and AIDS patients, African children, Brazilian Amerindians, and elderly individuals in certain regions of Europe. The aim of the present study was to determine the prevalence of HHV-8 antibodies in healthy children and young adults from different cities in São Paulo State, and in a population at high risk for HHV-8 infection: HIV-negative MSM, and AIDS patients with and without KS. Antibodies to HHV-8 latency-associated nuclear antigen and lytic-phase antigens were detected by immunofluorescence assays. In... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: HHV-8; Seroprevalence; AIDS; Kaposi's sarcoma; Immunofluorescence assay; Latency-associated nuclear antigen; Lytic-phase antigens. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100017 |
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