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	Provedor de dados 86 (1) Autor Bodanese‑Zanettini,Maria Helena (1) Weber,Ricardo Luís Mayer (1) Palavra-chave Composite plants (1) Genetic transformation (1) Glycine max (1) Hairy roots culture (1) Strain K599 (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2011 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Induction of transgenic hairy roots in soybean genotypes by Agrobacterium rhizogenes‑mediated transformation 86 Weber,Ricardo Luís Mayer; Bodanese‑Zanettini,Maria Helena. The objective of this work was to perform the screening of soybean genotypes as to their ability to respond to the induction of hairy roots by Agrobacterium rhizogenes‑mediated transformation. Four Brazilian soybean cultivars (BRSMG 68 Vencedora, BRS 137, Embrapa 48, and MG/BR 46 Conquista) and two North American ones adapted to Brazilian cropping conditions (Bragg and IAS‑5) were screened for their capacity to respond to A. rhizogenes in protocols for in vitro hairy root culture and ex vitro composite plant production. Four‑day‑old seedlings with uniform size were injected with A. rhizogenes harboring the plasmid p35S‑GFP. Seedlings expressing green fluorescent protein (GFP) in at least one hairy root were used to determine the transformation frequency.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Composite plants; Genetic transformation; Hairy roots culture; Strain K599. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000900014 Registros recuperados: 1 Primeira ... 1 ... Última

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