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| Torres,L.E.; Bima,P.; Maich,R.; Badiali,O.J.J.; Nisi,J.. |
| The aim of this work was to obtain haploid plants of bread wheat through wide hybridization with maize. The experimental material included ten bread wheat hybrids (female parent) and one population of maize (pollen donor). Two assays were carried out in two different seasons (summer and winter). Wheat spikes were manually emasculated, each spike was pollinated twice with fresh pollen of maize and a solution of 2,4-D (100 mg l-1) was sprayed on pollinated florets and injected in the upper internode. Fifteen and 21 days after pollination caryopses were removed and surface sterilized. Embryos were cultured in tubes containing B5 medium. The ten hybrid combinations produced caryopses, but only eight of these hybrids produced embryos and, in six of them, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bread wheat; Haploid plants; Wide hybridization; Maize pollen. |
| Ano: 2010 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2010000200003 |
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